LOCUS pC2HB-Far1 11520 bp DNA circular UNA 22-feb-2014 DEFINITION LMBP 11483. FEATURES Location/Qualifiers gene 321..2503 /label=CmLEU2 misc_feature 2741..3854 /feature_type="integratie sequentie" /label=IntegSeq Site 3248..3255 /site_type="restriction site" /label=NotI KanR 4499..5402 KanR 4528..4530 /label="KanR start codon" Site 5557..5562 /site_type="restriction site" /label=AatII promoter 5563..7002 /label=MET3p primer 6932..6950 /label=6372 Site join(7003..7008,7003^7004) /site_type="restriction site" /note="Name: BspEI" /note="Pattern: tccgga" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 7008^7009" /note="cut_0_on_negative_strand: 7012^7013" /note="inhibited_by: " /note="site_type: other" /note="restriction site" /label=BspEI misc_feature 7009..7011 /feature_type="Start codon" /label="Start codon" misc_signal 7021..7041 /label=SV40 CDS 7045..7662 /cds_type=ORF /label=LexA linker 7669..7686 /label="glycine linker" misc_feature 7687..7713 /feature_type="Ha tag" /label="HA tag" misc_feature 7714..7731 /feature_type=Misc. /label="Glycine linker" Site 7732..7737 /site_type="restriction site" /label=NheI Site 7738..7743 /site_type="restriction site" /label=StuI CDS 7744..10047 /cds_type=ORF /label=Far1 Site 10048..10053 /site_type="restriction site" /label=MluI Site join(10054..10059,10056^10057) /site_type="restriction site" /note="Name: ScaI" /note="Pattern: agtact" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 7772^7773" /note="cut_0_on_negative_strand: 7772^7773" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=ScaI terminator 10076..10589 /label=CaACT1term rep_origin 10719..11392 /label=pUCori ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 301 ctagtaacgg ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg 361 tcaagaaaga ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga 421 agctgcaaga ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc 481 tactaaattg aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa 541 cccaacatat caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc 601 tttgttaatg gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc 661 tttggaacca tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc 721 cattagattc ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc 781 aattaatgaa agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga 841 aggtattgac ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa 901 tttgtgttgt tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc 961 atgtatctga accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa 1021 cggcgtgcgt gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata 1081 tataataaac gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt 1141 ttttccttca tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta 1201 aaactattac aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca 1261 aagttttaga agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac 1321 atcatttaat cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac 1381 ttgaaagtgc caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg 1441 ggactggcgc cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt 1501 atgctaacat cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga 1561 gaccagaagt cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt 1621 atttcggtga tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg 1681 aaaaatacac cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac 1741 aacataaccc accattacca atctggtcat tggataaggc taatgtttta gcttcttcta 1801 gattatggag aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc 1861 aacatcaatt gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg 1921 gtataatcat cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc 1981 caggttcctt gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg 2041 cttttggtct ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta 2101 acccaattgc tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga 2161 aagaagctga agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg 2221 cagatttaag aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta 2281 ctaaaatctt aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta 2341 atacaaaatg aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat 2401 tgcgataatt caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat 2461 ctatttgaag taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg 2521 taagggcgaa ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta 2581 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac 2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa 2761 caaattacgt ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac 2821 ctgtaaaccg tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca 2881 taatccaaca aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca 2941 gagcgagcaa gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat 3001 ttcaagtgaa acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt 3061 tcctaacaag cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg 3121 gagtcaattt atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa 3181 tcctgagatt ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc 3241 cagcatagcg gccgctatgt gttcaaatat tgaaaccgtg aaaccagttt gatacagctg 3301 atattgtgtg ctaaaaaaag atgagcaccg aaagagtctt tagtttattg ccaagtaaag 3361 ggtcaaagaa tacctattct ttatgaacac agacttgctc taatggtgaa ttagtgtgga 3421 gtaatagaga aggaggcgaa acaaaaataa ttggttaaac actcgaatgt cacacaatcc 3481 taaataggtt aaattcagtt accgccgtga tgcgggaaca aagccgagcg gtaatatttt 3541 tggattctat gtttttttgg tgtatattgc acgacttggt gccaacaact ggtgttccaa 3601 cgcacaaaac taggttcaac tggatactgt tttctagaaa ttatttgcgg caattcatga 3661 ttacggtcct aaatttgaaa taaaagtttc caattatgtg tcttgacaat ttccattggg 3721 ttcaaaaaga tctagaatgt atgtaattac gcaacaattt gcatgcaaag aaataaaaac 3781 aaacctaaaa tccaactgct gttgttctgg tgatgatgta gtaaaataag agcacggcca 3841 agaacaaggt gatttacgta cggcagttta aggtttacac ctataaaaga gagagccgtt 3901 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga 3961 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 4021 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 4081 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 4141 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca 4201 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg 4261 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt 4321 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg 4381 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 4441 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat 4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 4681 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 5041 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 5281 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt 5341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga 5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 5461 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc 5521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcttccaatt agaggttgtg 5581 gatgcaaaat tgaaaaaaaa atgactattg gtagcgtgtt ctatttttct tatgcgctga 5641 aaaactacga acaattgtct attccaagcc tgtggctttt tttggtgatt cacaatacat 5701 aaattggcaa atacctcttc tatagacttt aacctttgat gaaatggtat ggataaacca 5761 cttttgtggt cagggtatat atatttttat tccaatctgg cctttataaa ttcaaatttc 5821 caaggggact ctgctaaacc ttctgatata actataactc taataatggc tgtaaaaagt 5881 gtgaaaaaaa gatgatattc acaaaaaaga taaaggggat ttcatattgg gtatctagat 5941 ttaaacatcg tatttcaaca atgaaactag aatcaatacg aaatccaggg cacaactgtg 6001 ttcattccct tgggaattat actgagtaat gggaagaatg tgcaaaaaat acccgtgtct 6061 tatataattt ccatttgtgt agggcttaaa agtggacaac ttcaggttac gaaaccacga 6121 aaatgtctaa acgtttgatc aaattcgtaa ctcatggaaa atcacagcaa caaattaagg 6181 atggatatat ccggtactta caaataaaaa cactaaagaa gtataaattt tgaactgaaa 6241 ttctatagaa aatctttttt tttgggtaaa attatgcgat tgtggctcat agtaacgtaa 6301 tcatacagtt caacaaacat cattttgtca aaggatttct aatcctattc tttttctact 6361 tggcttttga gtatatatat atatatatat acatcctttg ttcatcatca tgttcgattg 6421 aatttttttc ttggttgtta ttagattgtc tacttgagtt tttcttggtg caaattgtgg 6481 tgtttttcta acaaattttt caacatttaa agtatttctg gaacttttct acaaggaacg 6541 acatagatcc attaatgcgc ccctctaaaa caatacccat aatgtcttct taagtatgca 6601 tctgcttata aatgtttctg gatttacttt agttagaatg tcgacacgac aatgaactat 6661 tgctgtagat cacgtgcgat cactgaatta tgagtttctt tttatttttc tttttgggct 6721 tttttttttg caattttttt tttccacctt tgccaattgc gtagtcacat ataatgtata 6781 caatttttgc aactctgaca ctataattga tccaataata aagtatataa tagatcatca 6841 agtatacgta atctccccaa aaaaaaaatt gcataaaata aaatctataa acctttttta 6901 gatttttagt tgttcctttt ttctttctat tcttcaactt ccaagtgttg tcactttctt 6961 cctgttaact atatttaaaa gtaaataccc tccccagaaa actccggaat ggctgctcct 7021 ccaaaaaaga agagaaaggt agctagagaa ttaacaaaac gacaaagcga aatatataac 7081 tatattaaac aagttgttca aatgaaaggt tatccgccta gtgttcgcga aattggtgag 7141 gcagttggct tagcatccag ttcaactgtt catggtcacc tttcacgtct tgaagaaaaa 7201 ggctatataa gaagagatcc aacgaaacca cgtgctatag aaattgtaag tgatcaaaca 7261 aatgataata ttaatatgga agaaacgatt catgtgccag ttattggtaa agtcacagca 7321 ggtgttccta ttaccgcagt agaaaatatt gaagaatatt ttccattacc tgaacactta 7381 acatcgacac acaatagcga catattcata ttaaacgtcg taggcgacag tatgattgag 7441 gctggtatat tagacggaga caaagtaatt gttcgcagtc aaaccatagc agaaaatgga 7501 gacattattg ttgctatgac tgaggaagat gaagcaactg tcaaacgctt ctataaagaa 7561 aaaaatcgtt atcgattaca acctgaaaat agtacaatgg agccaattta cctcgacaat 7621 gttgctgtaa tcgggaaagt aattggtttg taccgcgaaa tgagatctgg tgctggtggt 7681 gttggttatc cgtatgatgt gcctgactac gcaggtgctg gtggtgttgg tgctagcAGG 7741 CCTATGCGCA AACTGTTCCA CTCTCCTAAA AAGAGAACTT CCACCTCAAC AAAGCTTGAA 7801 TCTATACTAC CTTTCCCAGC AGAGACTAAA AAGTATAATA ACATTAAATG TTCGTTGTGT 7861 CAAGAACTAC TAAGCTTGAC GTTGCCCGGA GAGCTTATTG TCCTGTTAGA GTGTGGCCAC 7921 ATGTCCCATA AAAACTGCTT GTCTTTAATA CAATCAGTGC CTGTTTGTAG TGAATGTAAT 7981 GTGATGTCGA ATTACGACTA CCCTCCATTC ACACCCGTGG ACCAGATTCA GTCGCCTGCC 8041 AAATTAAACA CTCAATTGGA AACACCATCC TTTACCTCGA GCGACCAAAC CATCTACAAT 8101 CCAAGAGTAA CATGTACCAG TGAGATTTCC GAAGTAACTT TAACCCCACC ATATGAAATA 8161 GCGTATGTGT TGAATGTTCA GGCTCCACTT ATTTTTAACG CTCATAAACC CACGCTAGAA 8221 GATTTACAGC TAAAAGAAAG GGTCGAAGAG TTTTTGAAGG TGCATCTTGA CACCGATAAG 8281 GATGTAGGAC AACTTGTTAT TTTTGATATA CTTGAAGTTA GTGTTACGGG CAAGAGCTGG 8341 GACCTGGCAT TGGTGTGTCT ATTTGAAAAC TACTTTTTAA TTTATGAAAA CGAGCTCTTG 8401 GTGGGAATAA TATCTGTTCA GCACGATATA AGCAGTGTTG ACGTGGATGA GGAACTCATT 8461 CTAAATCTAG CAAAAGATTC ACTTCCAGAA TTGCGACTCA GGCATTCAAA CAAGCTTGTG 8521 GTCCAAAAAT GGGGTACTCT ACTACTTGAA ATAATAGACA ATGAGAGTGT CGTCACTAAC 8581 ATTTACCAAT TGACTAATAC ATATTGGACC CACTTGCCCC CAGAGATTTG TGTACCACAA 8641 AATTTAACCA AACTAAACAA TGCAATCTTA TCCGGAACAA CCATTTCAAA TTCCGATATC 8701 GCAAGAATAC TACCAACACC TAAACAGCTA CTGTTAAATT TAATTCTTGC TCTACCACTA 8761 GTTAACCAAA CATCTTTAAC CGATCTAGAA TACAAAACAC AGATCCAACA AATCTTTCAA 8821 AAAACTTTAC ATGAACTCAG ACCAAATGAC AAGTTGGGAG TAATTTTTCT TGGAATAGAC 8881 GGGTCAAAAA AACCGTGTGC CAAAGGGTCC TTTGTTGGGT GCACTGAACC AAGCTGGAGT 8941 GAGTGGAAGC AGGTAATACG AGACATTACA ATAGTTCCAA ACTCATTTCA AAGTAACTTA 9001 CAGGAAATAA ATGTTGCTAT AGAAAAGTGT ATGGAGCTAT ACCCTTTTAT TCCACGTACA 9061 ACATCATCAG TTAACAAGTT TCTAATACTC AGCCCAAACA CATACTCACA GCTGCCAAAA 9121 GACGCACCCA AATCCAAATT TATTGACGCA ATAAACGAAA AACTATCTTG CACAATTGTT 9181 TGTGTCGGCA GCACATATGG CAACTACCAA CCATTCAAGT CTATTACTAC ATTTGGCACT 9241 CCTTTCCTAC GATTTGAGTC TTTCGCGAAG TTTGGCAGCT TACTAGTATA CTATATCAAC 9301 GAAAACCTCC ACAAAATATG TATTCCAAAA TTGACATTAA CATTAAAGGC ATCTCAGGGT 9361 TTTAGGTTCA CTGATCTTGA TTCCAGAGGG GCTATTGATG GAAGTCAGTT GACACTTACT 9421 ATTAAAGATA TTGTCCCACA GACGCAAAAA CTGATTGTGC TCAAAGTGCA AGCAAACCCC 9481 CAATTTGTTG CTTCCAAATA TCAGACGCTT CCAATATTTG AATATGATGG GTATTGGTTT 9541 TATGAGAAAT ACCTCGACAC CAAGTTGATA AGTACGAGAG TCAAGATAGG TGTCAGCCAG 9601 CAACCACCGA CACCTATTGA TGCGGAAGCA AGGGGAAGCT TTACGGAATA CTATTTAGAC 9661 ATCCCATTAC TACCTCCCGT GTCGCCAGCA AGAGATGCTG TATTTGCCAA AAGACAAACT 9721 GAGCTAACCA TAATTAACTC ATTAAAGAAA AGCATCGCGC TGGGTGAAAG CCAAGTGTCA 9781 CTGGATATAA TTAAAAGCTG CATATCGTTA GCACACGGAT TGATACGTAC TTCTCTTGAA 9841 GGCTCTACAA AATCACACAC CAATGAATCT AGCCCTGAGT GTGAGTATAG TATGTTACAG 9901 TTGCTAATGG CAGTAAGATA CACTGACAAA GGCAATGTTG AGTATATTGA TTTCTTGGTA 9961 AATCATATGA AAAGTATTGC CAAGCTACTA GAATCGGACC TCGATGCAGG TAAATTACGA 10021 TGTTCCGATT TGATGAATAG TTTAATAACG CGTagtactt aaccgcgccg tcgacgagtg 10081 aaattctgga aatctggaaa tctggttttg tattcttgtt attcttcttt ttgttattac 10141 atatataact tgttactttt ttaaaaaaat ctttgtttat tttataaata tataaaacta 10201 aatttaagaa aaagagaaaa atgttttatt tgagagattg aaattttact tgaatttagc 10261 ttagctttta taaagtatta ttatgtaaaa aaacaaaaca aatatacatt aaaaagttaa 10321 gactataaaa tagccaccca aggcatttct atatcttgtt gttgttgttt tcatcttctg 10381 tatcagagga acttatttta ttattttcgt cacgggtatt ttctcttgtt tgatgattca 10441 tcccattcat tccatcataa aattctagaa ggaccacctt tgattgtaaa tagtaataat 10501 taccaccctt atttaattat ttatttaact tatttattta tttattatac atatatacaa 10561 atctaataaa gtgaaaatct cccccttcac acgtggccga ggagcaggac tgacacgtgc 10621 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 10681 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 10741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 10801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 10861 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 10921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 10981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 11041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 11101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 11161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 11221 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 11281 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 11341 acgccagcaa cgcggccttt ttacggttcc tgggcttttg ctggcctttt gctcacatgt 11401 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 11461 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag //