LOCUS       pTT3-hL1CAM-bait        9935 bp    DNA     circular     17-JUN-2020
DEFINITION  LMBP11512.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 11512
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-JUN-2020
            NGS data implemented
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13389768|
COMMENT     VNTDBDATE|-13389842|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTT3-hL1CAM-bait|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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COMMENT                                   10.7904 0 -10 8.93864 1.1693 #124#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   -8.36393 -10.454 0 -10 3.96262 1.1693 #128#)) 
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COMMENT                                   -6.56519 0 -10 14.7851 1.1693 #131#)) 
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COMMENT                                    4.6157 4.66874 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 12 16711680) 8 1 4.6157 1.9 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of lacZ, incl. RBS" "@N" 1 18.7443 
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COMMENT                            "Last modified: 17-JUN-2020" 65535 0.0779533 
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COMMENT                            1.74233 0.611111 0 "NGS data implemented" 
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FEATURES             Location/Qualifiers
     CDS             647..1507
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      2048..2450
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        2542..2625
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             2626..2663
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        2939..3522
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             7446..7993
                     /vntifkey="4"
                     /label=rCd4d3+4
     terminator      8089..8124
                     /vntifkey="43"
                     /label=rabbit\ß-globin\polyA
     rep_origin      complement(8687..415)
                     /vntifkey="33"
                     /label=Epstein-Barr\oriP
     CDS             4083..7433
                     /vntifkey="4"
                     /label=hL1CAM
BASE COUNT     2545 a      2482 c      2572 g      2336 t 
ORIGIN
        1 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct 
       61 gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct atcctaattt 
      121 atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat 
      181 ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat 
      241 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat 
      301 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat 
      361 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag 
      421 ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata 
      481 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 
      541 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
      601 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
      661 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
      721 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
      781 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
      841 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg 
      901 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
      961 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     1021 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     1081 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     1141 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc 
     1201 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     1261 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     1321 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     1381 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     1441 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     1501 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     1561 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     1621 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     1681 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     1741 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     1801 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 
     1861 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     1921 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     1981 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     2041 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     2101 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     2161 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     2221 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     2281 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     2341 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 
     2401 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 
     2461 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 
     2521 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 
     2581 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 
     2641 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga 
     2701 ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg 
     2761 caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc 
     2821 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg 
     2881 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga 
     2941 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 
     3001 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 
     3061 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 
     3121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 
     3181 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 
     3241 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta 
     3301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg 
     3361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 
     3421 caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg 
     3481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag 
     3541 atcctcactc tcttccgcat cgctgtctgc gagggccagc tgttgggctc gcggttgagg 
     3601 acaaactctt cgcggtcttt ccagtactct tggatcggaa acccgtcggc ctccgaacgg 
     3661 tactccgcca ccgagggacc tgagcgagtc cgcatcgacc ggatcggaaa acctctcgag 
     3721 aaaggcgtct aaccagtcac agtcgcaagg taggctgagc accgtggcgg gcggcagcgg 
     3781 gtggcggtcg gggttgtttc tggcggaggt gctgctgatg atgtaattaa agtaggcggt 
     3841 cttgagacgg cggatggtcg aggtgaggtg tggcaggctt gagatccagc tgttggggtg 
     3901 agtactccct ctcaaaagcg ggcattactt ctgcgctaag attgtcagtt tccaaaaacg 
     3961 aggaggattt gatattcacc tggcccgatc tggccataca cttgagtgac aatgacatcc 
     4021 actttgcctt tctctccaca ggtgtccact cccaggtcca agtttaaact gcggccgcca 
     4081 ccatggtggt cgccctgcga tacgtgtggc ccctgctgct gtgctcccct tgcctgctgg 
     4141 gtaccattca gattccagaa gagtacgagg gccaccatgt catggagccc cctgtgatca 
     4201 ccgaacagag cccacggaga ctggtggtct tccccacaga cgatatttct ctgaaatgcg 
     4261 aggccagtgg caagcctgaa gtccagttcc gctggactcg agacggagtg cactttaagc 
     4321 ccaaagagga actgggcgtc accgtgtacc agtctcctca tagtggatca ttcacaatca 
     4381 ctggcaacaa tagtaacttc gcccagcggt ttcagggcat ctacagatgc tttgcatcta 
     4441 ataagctggg gacagccatg agtcacgaga tccggctgat ggccgaaggc gctcccaagt 
     4501 ggcctaaaga gactgtgaaa cccgtcgagg tggaggaagg ggaaagtgtg gtcctgccat 
     4561 gtaacccacc accttcagcc gagccactgc gaatctactg gatgaacagc aaaatcctgc 
     4621 acattaagca ggacgaaaga gtgacaatgg gacagaacgg caatctgtac ttcgcaaacg 
     4681 tgctgactag cgacaatcat tccgattata tttgccacgc ccattttcca ggcaccagaa 
     4741 caatcattca gaaggagccc atcgatctga gggtgaaagc caccaactct atgattgacc 
     4801 ggaagccaag actgctgttc cccacaaata gctcctctca cctggtcgcc ctgcagggac 
     4861 agccactggt gctggagtgt atcgctgaag gctttcccac tcctaccatc aagtggctgc 
     4921 gaccttccgg accaatgcca gcagacagag tgacctacca gaaccacaac aagaccctgc 
     4981 agctgctgaa ggtcggggag gaagacgatg gagagtatcg ctgcctggct gaaaactcac 
     5041 tgggcagcgc acgacatgcc tactatgtca ccgtggaggc cgctccttac tggctgcaca 
     5101 agccacagag ccatctgtat ggcccagggg agaccgccag gctggattgt caggtccagg 
     5161 gccgccctca gccagaagtg acatggcgaa tcaacgggat tccagtggag gaactggcta 
     5221 aggatcagaa atacagaatc cagcgcggag cactgattct gagtaatgtc cagccctcag 
     5281 acactatggt gacccagtgc gaggcaagga accgccacgg cctgctgctg gcaaatgcct 
     5341 acatctatgt cgtccagctg cccgcaaaga ttctgacagc cgataaccag acttacatgg 
     5401 ccgtgcaggg gtccaccgct tatctgctgt gcaaggcctt cggcgcacca gtcccttctg 
     5461 tgcagtggct ggacgaggat gggaccacag tgctgcagga cgaacggttc tttccctacg 
     5521 ccaacgggac actgggaatc agggacctgc aggctaatga tactggacgc tatttttgcc 
     5581 tggcagccaa cgatcagaac aatgtcacca ttatggctaa tctgaaggtg aaagacgcaa 
     5641 cacagatcac tcagggccct cgaagcacaa ttgagaagaa agggtcccgg gtgacattca 
     5701 cttgtcaggc ctcttttgac cctagtctgc agccatcaat cacctggaga ggagacggca 
     5761 gggatctgca ggagctgggg gacagcgata agtacttcat cgaagatggc cggctggtca 
     5821 tccacagcct ggactactcc gatcagggca actattcttg cgtggcaagt accgagctgg 
     5881 acgtggtcga atcccgagca cagctgctgg tggtcggctc tccaggacca gtccctagac 
     5941 tggtgctgtc agatctgcac ctgctgacac agagccaggt cagggtgagc tggtcccctg 
     6001 ctgaggacca taatgcacca atcgaaaagt acgacattga gttcgaagat aaagagatgg 
     6061 cccctgaaaa gtggtattcc ctgggaaaag tgccaggcaa ccagacctca actaccctga 
     6121 agctgagccc atacgtccac tatacctttc gcgtgacagc catcaacaag tacggaccag 
     6181 gagagccatc ccccgtgtct gaaaccgtgg tcacacccga ggctgcacct gaaaagaacc 
     6241 cagtcgatgt gaaaggcgag gggaacgaaa caactaatat ggtcatcacc tggaagcctc 
     6301 tgaggtggat ggactggaat gccccacagg tccagtaccg agtgcagtgg cgaccccagg 
     6361 gaacacgagg accttggcag gagcagatcg tgagcgaccc attcctggtg gtcagtaaca 
     6421 cctcaacatt tgtgccctat gaaattaagg tccaggctgt gaacagccag ggaaaaggcc 
     6481 ctgagccaca ggtgaccatc gggtacagtg gagaagatta tccccaggca attcctgagc 
     6541 tggaaggcat cgagattctg aacagttcag ctgtcctggt gaagtggagg cccgtcgacc 
     6601 tggcacaggt gaaagggcac ctgcgcggat acaatgtgac atattggcgg gagggcagcc 
     6661 agagaaagca ctccaaaagg cacatccata aggatcatgt ggtcgtgccc gccaacacca 
     6721 catcagtgat tctgagcggg ctgaggcctt acagctccta tcatctggag gtgcaggcat 
     6781 tcaatggacg aggcagcgga ccagcctctg agttcacttt ttccacccct gaaggagtgc 
     6841 caggacaccc agaggccctg catctggaat gtcagagcaa cacttccctg ctgctgaggt 
     6901 ggcagccacc cctgagccac aatggcgtcc tgaccggcta cgtgctgtcc tatcatccac 
     6961 tggatgaggg agggaaggga cagctgagct tcaacctgcg cgatcctgaa ctgcgaactc 
     7021 acaatctgac cgacctgtcc ccacatctgc gataccggtt tcagctgcag gctactacca 
     7081 aagaggggcc tggagaagca atcgtgcggg agggaggcac aatggccctg tctggcatca 
     7141 gtgacttcgg gaacattagc gccactgcag gagagaatta ctcagtcgtg agctgggtgc 
     7201 caaaggaagg ccagtgcaac ttcagatttc acatcctgtt taaggccctg ggcgaggaaa 
     7261 aagggggagc ttctctgagt cctcagtacg tgtcctataa tcagtctagt tacactcagt 
     7321 gggatctgca gccagacacc gattatgaga tccacctgtt caaggaacga atgtttcggc 
     7381 atcagatggc cgtcaaaaca aacggcactg ggagagtgag gctgcctcca gcaggcgcgc 
     7441 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac 
     7501 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt 
     7561 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta 
     7621 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg 
     7681 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt 
     7741 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct 
     7801 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg 
     7861 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt 
     7921 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat 
     7981 ccaaagggtt gaattccgga tcactgcatc atattctgga tgcacagaaa atggtgtgga 
     8041 atcatcgtta agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta 
     8101 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 
     8161 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg 
     8221 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg 
     8281 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac 
     8341 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat 
     8401 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt 
     8461 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca 
     8521 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt 
     8581 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag 
     8641 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta 
     8701 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct 
     8761 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct 
     8821 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac 
     8881 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt 
     8941 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat 
     9001 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg 
     9061 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa 
     9121 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg 
     9181 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata 
     9241 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag 
     9301 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca 
     9361 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg 
     9421 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc 
     9481 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg 
     9541 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc 
     9601 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg 
     9661 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct 
     9721 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta 
     9781 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct 
     9841 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct 
     9901 aatttatatc tgggtagcat aggctatcct aatct 
//
