LOCUS AVEXIS_Bio_PSG1_ 8588 bp ds-DNA circular SYN 30-MAR-2017 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8588) AUTHORS Lisa TITLE Direct Submission JOURNAL Exported Sep 9, 2019 from SnapGene Viewer 4.2.3 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|801619200| COMMENT VNTDBDATE|801619200| COMMENT VNTNAME|AVEXIS_Bio_PSG1_hSidekick2 6FNs.dna| COMMENT VNTAUTHORNAME|Lisa| FEATURES Location/Qualifiers misc_feature 8..552 /label=rat CD4 domain 3 and 4 primer complement(221..240) /label=211 primer rev /note="sequence: acctggggtatctgaagggt" primer complement(641..659) /label=1367 rev primer /note="sequence: tagccagaggtcgaggtcg" polyA_site 659..714 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" promoter 3036..3140 /gene="bla" /label=AmpR promoter CDS 3141..4001 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 4172..4760 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 5048..5069 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5084..5114 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5122..5138 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5146..5162 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" enhancer 5433..5812 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5813..6016 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer 6374..6393 /label=212 primer fw /note="sequence: tgagatccagctgttggggt" sig_peptide 6574..6675 /label=PSG1 misc_feature 6682..8556 /label=hSidekick2 CDS 8563..8580 /codon_start=1 /product="6xHis affinity tag" /label=6xHis ORIGIN 1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact 61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc 121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac 181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt 241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta 301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg 361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga 421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg 481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc 541 caaagggttg aattccggat cactgcatca tattctggat gcacagaaaa tggtgtggaa 601 tcatcgttaa gtctagacac gtgattaatt aaggatcccc cgacctcgac ctctggctaa 661 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga 721 aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct agctagagga 781 tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg 841 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca 901 tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata 961 aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc 1021 tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa 1081 tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc 1141 aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt 1201 gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat 1261 atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta 1321 tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg 1381 tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca 1441 agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc 1501 ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg 1561 tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt 1621 tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa 1681 tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg 1741 acaagccgta aagactggat gtccatctca cacgaattta tggctatggg caacacataa 1801 tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg 1861 tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag 1921 cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg 1981 gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg 2041 tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc 2101 tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca 2161 ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga 2221 tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc 2281 atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac 2341 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata ggctatccta 2401 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta 2461 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta 2521 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta 2581 atagagatta gggtagtata tgctatccta atttatatct gggtagcata tactacccaa 2641 atatctggat agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct 2701 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct 2761 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct 2821 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct 2881 gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa ttttcttgaa 2941 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 3001 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 3061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 3121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 3181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 3241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 3301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 3361 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 3421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 3481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 3541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 3601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 3661 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 3721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 3781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 3841 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 3901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 3961 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 4021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 4081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 4141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 4201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 4261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 4321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 4381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 4441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 4501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 4561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 4621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 4681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 4741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 4801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 4861 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 4921 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4981 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 5041 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 5101 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 5161 accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg acagcttatc 5221 atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa 5281 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata 5341 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta 5401 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata 5461 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 5521 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 5581 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 5641 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc 5701 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg 5761 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 5821 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 5881 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 5941 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt 6001 ctatataagc agagctcgtt tagtgaaccg tcagatcctc actctcttcc gcatcgctgt 6061 ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac tcttcgcggt ctttccagta 6121 ctcttggatc ggaaacccgt cggcctccga acggtactcc gccaccgagg gacctgagcg 6181 agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc 6241 aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg gtcggggttg tttctggcgg 6301 aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgaggtga 6361 ggtgtggcag gcttgagatc cagctgttgg ggtgagtact ccctctcaaa agcgggcatt 6421 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc 6481 gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc cacaggtgtc 6541 cactcccagg tccaagttta aactgcggcc gccatgggaa ccctctcagc ccctccctgc 6601 acacagcgca tcaaatggaa ggggctcctg ctcacagcat cacttttaaa cttctggaac 6661 ctgcccacca ctgccggtac cgtccctgga ccccctatgg gcatcctgtt tcccgaagtg 6721 agaaccacaa gcgtgagact gatttggcag cctcccgccg cacctaatgg catcatcctg 6781 gcctaccaga tcacccacag gctgaacacc acaaccgcca atacagccac cgtggaggtg 6841 ctggcacctt ccgccaggca gtacacagca accggcctga agccagagag cgtgtacctg 6901 ttccggatca cagcccagac cagaaaggga tggggagagg cagcagaggc cctggtggtg 6961 acaaccgaga agagggatag gccacagcca ccttccaggc ctatggtgca gcaggaggac 7021 gtgcgggcca gatctgtgct gctgagctgg gagccaggat ccgatggact gtctcccgtg 7081 agatactata caatccagac cagggagctg ccatctggcc gctgggcact gcacagcgcc 7141 tccgtgtctc acaacgccag ctccttcatc gtggaccggc tgaagccttt cacaagctac 7201 aagtttagag tgaaggccac caatgacatc ggcgattctg agtttagcga ggagagcgag 7261 tccctgacaa ccctgcaggc agcaccagat gaggcaccaa caatcctgtc cgtgacccca 7321 cacacaacca catctgtgct gatcaggtgg cagccaccag cagaggacaa gatcaacggc 7381 atcctgctgg gcttcaggat ccgctaccgg gagctgctgt atgagggcct gaggggcttt 7441 acactgcgcg gcatcaacaa tccaggagca acatgggcag agctgacctc catgtactct 7501 atgcggaatc tgagcagacc ctccctgacc cagtatgagc tggataacct gaataagcac 7561 cggagatacg agatcaggat gagcgtgtat aacgcagtgg gagagggacc ctctagccct 7621 ccacaggagg tgttcgtggg agaggccgtg cctacagcag caccaaggaa tgtggtggtg 7681 cacggagcaa cagcaaccca gctggacgtg acctgggagc cacctccact ggactcccag 7741 aacggcgata tccagggcta caagatctat ttctgggagg cccagcgggg caatctgaca 7801 gagagagtga agaccctgtt tctggccgag aacagcgtga agctgaagaa tctgacaggc 7861 tacaccgcct atatggtgag cgtggcagcc tttaacgcag caggcgatgg accacggtcc 7921 acacctaccc agggacagac ccagcaggca gcaccaagcg ccccctcctc tgtgaagttc 7981 tccgagctga ccacaaccag cgtgaacgtg tcctgggagg cccctcagtt tccaaatggc 8041 atcctggagg gctacagact ggtgtatgag ccctgctctc ctgtggatgg cgtgagcaag 8101 atcgtgacag tggacgtgaa gggcaactcc cctctgtggc tgaaggtgaa ggatctggcc 8161 gagggcgtga cctacagatt caggatccgc gccaagacct tcacctatgg ccctgagatc 8221 gaggccaatg tgacaaccgg acctggagag ggcgccccag gaccacctgg cgtgccaatc 8281 atcgtgaggt acagctccgc catcgccatc cactggtcta gcggcgaccc aggcaaggga 8341 ccaatcacca gatatgtgat cgaggcaagg ccaagcgacg agggactgtg ggatatcctg 8401 atcaaggaca tccctaagga ggtgtcctct tacaccttct ctatggatat cctgaagccc 8461 ggcgtgagct atgactttcg cgtgatcgcc gtgaatgatt acgggttcgg gacaccttca 8521 agccccagtc agtcagtgcc tgcccagaaa gcaaacgagc tccaccacca ccaccaccat 8581 ggcgcgcc //