LOCUS AVEXIS_beta_PSG1 8931 bp ds-DNA circular SYN 10-APR-2017 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8931) AUTHORS Lisa TITLE Direct Submission JOURNAL Exported Sep 9, 2019 from SnapGene Viewer 4.2.3 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|802656000| COMMENT VNTDBDATE|802656000| COMMENT VNTNAME|AVEXIS_beta_PSG1_hNetrin11 WT.dna| COMMENT VNTAUTHORNAME|Lisa| FEATURES Location/Qualifiers misc_feature 8..552 /label=rat CD4 domain 3 and 4 primer complement(221..240) /label=211 primer rev /note="sequence: acctggggtatctgaagggt" CDS 767..1555 /codon_start=1 /gene="bla(M)" /product="beta-lactamase lacking the signal sequence" /label=bla(M) /note="allows cytosolic expression of beta-lactamase" primer complement(1572..1590) /label=1367 rev primer /note="sequence: tagccagaggtcgaggtcg" polyA_site 1590..1645 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" promoter 3967..4071 /gene="bla" /label=AmpR promoter CDS 4072..4932 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 5103..5691 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 5979..6000 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6015..6045 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6053..6069 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6077..6093 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" enhancer 6364..6743 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 6744..6947 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer 6898..6918 /label=CMV fw /note="sequence: cgcaaatgggcggtaggcgtg" primer 7305..7324 /label=212 primer fw /note="sequence: tgagatccagctgttggggt" sig_peptide 7505..7606 /label=PSG1 misc_feature 7613..8899 /label=Netrin-11WT CDS 8906..8923 /codon_start=1 /product="6xHis affinity tag" /label=6xHis ORIGIN 1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact 61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc 121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac 181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt 241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta 301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg 361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga 421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg 481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc 541 caaagggttg aattccggcg gcggctccgg aggcggtacc ggtggcggcg acctagcccc 601 acagatgctt cgagaactcc aggagactaa tgcggcgctg caagacgtga gagagctctt 661 gcgacagcag gtcaaggaga tcaccttcct gaagaatacg gtgatggaat gtgacgcctg 721 cggtctagac agaaacctgc ctccactagc acctctgggc ccacacccag aaacgctggt 781 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 841 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 901 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 961 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 1021 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 1081 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 1141 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 1201 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 1261 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 1321 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 1381 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 1441 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 1501 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaattaat 1561 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt 1621 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt 1681 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct 1741 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg 1801 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag 1861 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag 1921 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat 1981 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc 2041 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata 2101 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa 2161 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata 2221 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa 2281 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat 2341 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg 2401 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat 2461 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg 2521 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa 2581 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat 2641 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc 2701 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt 2761 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa 2821 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc 2881 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct 2941 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt 3001 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc 3061 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg 3121 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc 3181 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg 3241 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct 3301 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct 3361 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct 3421 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct 3481 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct 3541 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc 3601 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc 3661 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt 3721 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc 3781 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg 3841 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt 3901 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 3961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 4021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 4081 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 4141 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 4201 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 4261 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac 4321 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 4381 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 4441 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 4501 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 4561 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca 4621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 4681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 5041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 5101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 5161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 5221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 5281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 5341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 5401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 5461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 5521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 5581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 5701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 5761 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 5821 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 5881 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 5941 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 6001 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 6061 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag 6121 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat 6181 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat 6241 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata 6301 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat 6361 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 6421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 6481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 6541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 6601 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg 6661 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg 6721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat 6781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 6841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc 6901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc 6961 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg ggctcgcggt 7021 tgaggacaaa ctcttcgcgg tctttccagt actcttggat cggaaacccg tcggcctccg 7081 aacggtactc cgccaccgag ggacctgagc gagtccgcat cgaccggatc ggaaaacctc 7141 tcgagaaagg cgtctaacca gtcacagtcg caaggtaggc tgagcaccgt ggcgggcggc 7201 agcgggtggc ggtcggggtt gtttctggcg gaggtgctgc tgatgatgta attaaagtag 7261 gcggtcttga gacggcggat ggtcgaggtg aggtgtggca ggcttgagat ccagctgttg 7321 gggtgagtac tccctctcaa aagcgggcat tacttctgcg ctaagattgt cagtttccaa 7381 aaacgaggag gatttgatat tcacctggcc cgatctggcc atacacttga gtgacaatga 7441 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaagttt aaactgcggc 7501 cgccatggga accctctcag cccctccctg cacacagcgc atcaaatgga aggggctcct 7561 gctcacagca tcacttttaa acttctggaa cctgcccacc actgccggta ccggacctgg 7621 gctgtcaatg tttgcaggac aggcagctca gcccgaccct tgctctgatg agaacgggca 7681 ccctcggaga tgtatcccag acttcgtgaa tgcagccttt ggaaaggatg tgagagtcag 7741 ctccacctgc ggcagacccc ctgctaggta ctgcgtggtg agcgaaaggg gcgaggaacg 7801 cctgcgatct tgccacctgt gcaacgcaag tgacccaaag aaagctcatc cacccgcatt 7861 cctgaccgat ctgaacaatc cccacaatct gacatgctgg cagtctgaga actacctgca 7921 gtttcctcat aatgtgacac tgactctgag tctggggaag aaattcgagg tgacctacgt 7981 gagcctgcag ttttgtagtc caagacccga atcaatggcc atctacaaga gcatggacta 8041 tggcagaact tgggtgccat tccagtttta ctccacccag tgcaggaaaa tgtataaccg 8101 accccaccgg gcacctatca caaagcagaa tgagcaggaa gccgtgtgca cagacagcca 8161 tactgatatg cgccccctgt caggcgggct gattgctttc agcactctgg acggccggcc 8221 atccgcacac gactttgata actctcccgt gctgcaggac tgggtcaccg caacagatat 8281 tagagtggcc ttctccaggc tgcatacctt tggcgatgag aatgaagacg attcagagct 8341 ggctcgggac agctactttt atgcagtgtc cgatctgcag gtcggaggca ggtgcaaatg 8401 taatggacac gctgcaagat gcgtgcggga cagagacgat agcctggtct gcgattgtag 8461 acacaatact gccgggcctg aatgcgacag gtgtaagccc ttccattacg atagaccttg 8521 gcagagagct acagcaagag aggcaaacga atgcgtggct tgcaactgta atctgcacgc 8581 caggcgctgt cgctttaata tggagctgta caaactgagt ggacggaagt cagggggagt 8641 gtgcctgaac tgtcggcata atacagctgg cagacactgc cattattgta aagaagggta 8701 ctatcgagac atgggaaagc ctatcaccca caggaaagcc tgcaaggctt gcgattgtca 8761 tccagtgggc gccgctggga aaacatgtaa ccagaccaca gggcagtgcc cctgtaagga 8821 cggagtcact ggcatcacct gcaatcgctg tgccaaagga taccagcagt cccggtctcc 8881 tattgcccca tgcatcaagg agctccacca ccaccaccac catggcgcgc c //