LOCUS       pTT3-hDCC-prey         10659 bp    DNA     circular     17-JUN-2020
DEFINITION  LMBP 11518.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 11518
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-JUN-2020
            NGS data implemented
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13389883|
COMMENT     VNTDBDATE|-13389919|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTT3-hDCC-prey|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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FEATURES             Location/Qualifiers
     CDS             747..1607
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      2148..2550
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        2642..2725
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             2726..2763
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        3039..3622
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     terminator      9063..9098
                     /vntifkey="43"
                     /label=rabbit\ß-globin\polyA
     rep_origin      complement(9661..515)
                     /vntifkey="33"
                     /label=Epstein-Barr\oriP
     CDS             7489..8036
                     /vntifkey="4"
                     /label=rCd4d3+4
     CDS             4183..7476
                     /vntifkey="4"
                     /label=hDCC
     CDS             8037..9039
                     /vntifkey="4"
                     /label=rCOMP
BASE COUNT     2808 a      2552 c      2632 g      2656 t        11 others
ORIGIN
        1 tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca 
       61 cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat agcatatgct 
      121 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct 
      181 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct 
      241 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatsct 
      301 awyctatatc tgkrtmcgsr tagsmtatsc taatctatat ctgggtagca tatgctatcc 
      361 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc 
      421 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc 
      481 taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca tgagaatttt 
      541 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 
      601 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
      661 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
      721 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
      781 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
      841 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
      901 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
      961 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc 
     1021 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     1081 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     1141 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     1201 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     1261 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat 
     1321 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     1381 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     1441 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     1501 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     1561 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     1621 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     1681 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     1741 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     1801 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     1861 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     1921 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     1981 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     2041 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     2101 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     2161 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     2221 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     2281 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     2341 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     2401 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 
     2461 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 
     2521 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 
     2581 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 
     2641 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 
     2701 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 
     2761 gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca tgtttgacag 
     2821 cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga actggtaggt 
     2881 atggaagatc tatacattga atcaatattg gcaattagcc atattagtca ttggttatat 
     2941 agcataaatc aatattggct attggccatt gcatacgttg tatctatatc ataatatgta 
     3001 catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta 
     3061 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 
     3121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 
     3181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 
     3241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc 
     3301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 
     3361 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 
     3421 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc 
     3481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt 
     3541 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg 
     3601 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcctcactc tcttccgcat 
     3661 cgctgtctgc gagggccagc tgttgggctc gcggttgagg acaaactctt cgcggtcttt 
     3721 ccagtactct tggatcggaa acccgtcggc ctccgaacgg tactccgcca ccgagggacc 
     3781 tgagcgagtc cgcatcgacc ggatcggaaa acctctcgag aaaggcgtct aaccagtcac 
     3841 agtcgcaagg taggctgagc accgtggcgg gcggcagcgg gtggcggtcg gggttgtttc 
     3901 tggcggaggt gctgctgatg atgtaattaa agtaggcggt cttgagacgg cggatggtcg 
     3961 aggtgaggtg tggcaggctt gagatccagc tgttggggtg agtactccct ctcaaaagcg 
     4021 ggcattactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc 
     4081 tggcccgatc tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca 
     4141 ggtgtccact cccaggtcca agtttaaact gcggccgcca ccatggagaa tagtcttaga 
     4201 tgtgtttggg tacccaagct ggcttttgta ctcttcggag cttccttgct cagcgcgcat 
     4261 cttcaagtaa ccggttttca aattaaagct ttcacagcac tgcgcttcct ctcagaacct 
     4321 tctgatgccg tcacaatgcg gggaggaaat gtcctcctcg actgctccgc ggagtccgac 
     4381 cgaggagttc cagtgatcaa gtggaagaaa gatggcattc atctggcctt gggaatggat 
     4441 gaaaggaagc agcaactttc aaatgggtct ctgctgatac aaaacatact tcattccaga 
     4501 caccacaagc cagatgaggg actttaccaa tgtgaggcat ctttaggaga ttctggctca 
     4561 attattagtc ggacagcaaa agttgcagta gcaggaccac tgaggttcct ttcacagaca 
     4621 gaatctgtca cagccttcat gggagacaca gtgctactca agtgtgaagt cattggggag 
     4681 cccatgccaa caatccactg gcagaagaac caacaagacc tgactccaat cccaggtgac 
     4741 tcccgagtgg tggtcttgcc ctctggagca ttgcagatca gcggactcca accgggggac 
     4801 attggaattt accgatgctc agctcgaaat ccagccagct caagaacagg aaatgaagca 
     4861 gaagtcagaa ttttatcaga tccaggactg catagacagc tgtattttct gcaaagacca 
     4921 tccaatgtag tagccattga aggaaaagat gctgtcctgg aatgttgtgt ttctggctat 
     4981 cctccaccaa gttttacctg gttacgaggc gaggaagtca tccaactcag gtctaaaaag 
     5041 tattctttat tgggtggaag caacttgctt atctccaatg tgacagatga tgacagtgga 
     5101 atgtatacct gtgttgtcac atataaaaat gagaatatta gtgcctctgc agagctcaca 
     5161 gtcttggttc cgccatggtt tttaaatcat ccttccaacc tgtatgccta tgaaagcatg 
     5221 gatattgagt ttgaatgtac agtctctgga aagcctgtgc ccactgtgaa ttggatgaag 
     5281 aatggagatg tggtcattcc tagtgattat tttcagatag tgggaggaag caacttacgg 
     5341 atacttgggg tggtgaagtc agatgaaggc ttttatcaat gtgtggctga aaatgaggct 
     5401 ggaaatgccc agaccagtgc acagctcatt gtccctaagc ctgctatccc aagctccagt 
     5461 gtcctccctt cggctcccag agatgtggtc cctgtcttgg tttccagccg atttgtccgt 
     5521 ctcagctggc gcccacctgc agaagcgaaa gggaacattc aaactttcac ggtctttttc 
     5581 tccagagaag gtgacaacag ggaacgagca ttgaatacaa cacagcctgg gtcccttcag 
     5641 ctcactgtgg gaaacctgaa gccagaagcc atgtacacct ttcgagttgt ggcttacaat 
     5701 gaatggggac cgggagagag ttctcaaccc atcaaggtgg ccacacagcc tgagttgcaa 
     5761 gttccagggc cagtagaaaa cctgcaagct gtatctacct cacctacctc aattcttatt 
     5821 acctgggaac cccctgccta tgcaaacggt ccagtccaag gttacagatt gttctgcact 
     5881 gaggtgtcca caggaaaaga acagaatata gaggttgatg gactatctta taaactggaa 
     5941 ggcctgaaaa aattcaccga atatagtctt cgattcttag cttataatcg ctatggtccg 
     6001 ggcgtctcta ctgatgatat aacagtggtt acactttctg acgtgccaag tgccccgcct 
     6061 cagaacgtct ccctggaagt ggtcaattca agaagtatca aagttagctg gctgcctcct 
     6121 ccatcaggaa cacaaaatgg atttattacc ggctataaaa ttcgacacag aaagacgacc 
     6181 cgcaggggtg agatggaaac actggagcca aacaacctct ggtacctatt cacaggactg 
     6241 gagaaaggaa gtcagtacag tttccaggtg tcagccatga cagtcaatgg tactggacca 
     6301 ccttccaact ggtatactgc agagactcca gagaatgatc tagatgaatc tcaagttcct 
     6361 gatcaaccaa gctctcttca tgtgaggccc cagactaact gcatcatcat gagttggact 
     6421 cctcccttga acccaaacat cgtggtgcga ggttatatta tcggttatgg cgttgggagc 
     6481 ccttacgctg agacagtgcg tgtggacagc aagcagcgat attattccat tgagaggtta 
     6541 gagtcaagtt cccattatgt aatctcccta aaagctttta acaatgccgg agaaggagtt 
     6601 cctctttatg aaagtgccac caccaggtct ataaccgatc ccactgaccc agttgattat 
     6661 tatcctttgc ttgatgattt ccccacctcg gtcccagatc tctccacccc catgctccca 
     6721 ccagtaggtg tacaggctgt ggctcttacc catgatgctg tgagggtcag ctgggcagac 
     6781 aactctgtcc ctaagaacca aaagacgtct gaggtgcgac tttacaccgt ccggtggaga 
     6841 accagctttt ctgcaagtgc aaaatacaag tcagaagaca caacatctct aagttacaca 
     6901 gcaacaggcc tcaaaccaaa cacaatgtat gaattctcgg tcatggtaac aaaaaacaga 
     6961 aggtccagta cttggagcat gactgcacat gccaccacgt atgaagcagc ccccacctct 
     7021 gctcccaagg acttgacagt cattactagg gaagggaagc ctcgtgccgt cattgtgagt 
     7081 tggcagcctc ccttggaagc caatgggaaa attactgctt acatcttatt ttataccttg 
     7141 gacaagaaca tcccaattga tgactggatt atggaaacaa tcagtggtga taggcttact 
     7201 catcaaatca tggatctcaa ccttgatact atgtattact ttcgaattca agcacgaaat 
     7261 tcaaaaggag tggggccact ctctgatcct atcctcttca ggactctgaa agtggaacac 
     7321 cctgacaaaa tggctaatga ccaaggtcgt catggagatg gaggttattg gccagttgat 
     7381 actaatttga ttgatagaag caccctaaat gagccgccaa ttggacaaat gcaccccccg 
     7441 catggcagtg tcactcctca gaagaacagc aacctgggcg cgccgtcgac ctccatcacg 
     7501 gcctataaga gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa 
     7561 agcctgcagg gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc 
     7621 accttctccc taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc 
     7681 cagctgtccg aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct 
     7741 ggttctggca acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg 
     7801 gtggtgatga aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc 
     7861 acctcaccca agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg 
     7921 caggagaaag tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt 
     7981 gaaggtgaag aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc 
     8041 ggcggcggct ccggaggcgg taccggtggc ggcgacctag ccccacagat gcttcgagaa 
     8101 ctccaggaga ctaatgcggc gctgcaagac gtgagagagc tcttgcgaca gcaggtcaag 
     8161 gagatcacct tcctgaagaa tacggtgatg gaatgtgacg cctgcggtct agacagaaac 
     8221 ctgcctccac tagcacctct gggcccacac ccagaaacgc tggtgaaagt aaaagatgct 
     8281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 
     8341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 
     8401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 
     8461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 
     8521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 
     8581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 
     8641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 
     8701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 
     8761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 
     8821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 
     8881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 
     8941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 
     9001 atcgctgaga taggtgcctc actgattaag cattggtaat taattaagga tcccccgacc 
     9061 tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 
     9121 tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat ccctcggaga 
     9181 tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg 
     9241 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact 
     9301 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta 
     9361 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga 
     9421 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg 
     9481 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga 
     9541 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag 
     9601 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt 
     9661 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac 
     9721 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc 
     9781 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga 
     9841 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga 
     9901 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga 
     9961 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa 
    10021 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac 
    10081 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa 
    10141 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct 
    10201 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc 
    10261 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt 
    10321 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg 
    10381 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg 
    10441 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg 
    10501 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca 
    10561 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg 
    10621 gcgcgattgc tgcgatctgg aggacaaatt acacacact 
//
