LOCUS       AVEXIS_1° beta_D       10665 bp ds-DNA     circular SYN 14-JUL-2017
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Lisa
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2019 from SnapGene Viewer 4.2.3 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     VNTDATE|801360000|
COMMENT     VNTDBDATE|811296000|
COMMENT     VNTNAME|AVEXIS_1(o) beta_DCC ecd (z297).dna|
COMMENT     VNTAUTHORNAME|Lisa|
FEATURES             Location/Qualifiers
     misc_feature    8..552
                     /label=rat CD4 domain 3 and 4
     primer          complement(221..240)
                     /label=211 primer rev
                     /note="sequence: acctggggtatctgaagggt"
     CDS             623..1411
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
     primer          complement(1428..1446)
                     /label=1367 rev primer
                     /note="sequence: tagccagaggtcgaggtcg"
     polyA_site      1446..1501
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        3823..3927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3928..4788
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      4959..5547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5835..5856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5871..5901
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5909..5925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5933..5949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        6220..6599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6600..6803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer          7161..7180
                     /label=212 primer fw
                     /note="sequence: tgagatccagctgttggggt"
     misc_feature    7364..10657
                     /label=hDCC ecd
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggcg gcggctccgg aggcggtacc ctagacagaa acctgcctcc
      601 actagcacct ctgggcccac acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1381 gataggtgcc tcactgatta agcattggta attaattaag gatcccccga cctcgacctc
     1441 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     1501 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     1561 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     1621 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     1681 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     1741 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     1801 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     1861 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     1921 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     1981 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     2041 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     2101 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     2161 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     2221 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     2281 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     2341 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     2401 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     2461 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     2521 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     2581 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     2641 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     2701 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     2761 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     2821 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     2881 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     2941 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     3001 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     3061 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     3121 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     3181 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     3241 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     3301 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     3361 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     3421 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     3481 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     3541 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     3601 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     3661 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     3721 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3781 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3841 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3901 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3961 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4021 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4081 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4141 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     4201 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4261 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4321 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4381 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4441 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     4501 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4561 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4621 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4681 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4741 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4801 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4861 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4921 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4981 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5041 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5101 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5161 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5221 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5281 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5341 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5461 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5521 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5581 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5641 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5701 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5761 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5821 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     5881 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     5941 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     6001 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     6061 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     6121 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     6181 acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt
     6241 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     6301 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     6361 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     6421 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     6481 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     6541 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     6601 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     6661 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     6721 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     6781 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcctcact ctcttccgca
     6841 tcgctgtctg cgagggccag ctgttgggct cgcggttgag gacaaactct tcgcggtctt
     6901 tccagtactc ttggatcgga aacccgtcgg cctccgaacg gtactccgcc accgagggac
     6961 ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca
     7021 cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggtggcggtc ggggttgttt
     7081 ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc
     7141 gaggtgaggt gtggcaggct tgagatccag ctgttggggt gagtactccc tctcaaaagc
     7201 gggcattact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac
     7261 ctggcccgat ctggccatac acttgagtga caatgacatc cactttgcct ttctctccac
     7321 aggtgtccac tcccaggtcc aagtttaaac tgcggccgcc accatggaga atagtcttag
     7381 atgtgtttgg gtacccaagc tggcttttgt actcttcgga gcttccttgc tcagcgcgca
     7441 tcttcaagta accggttttc aaattaaagc tttcacagca ctgcgcttcc tctcagaacc
     7501 ttctgatgcc gtcacaatgc ggggaggaaa tgtcctcctc gactgctccg cggagtccga
     7561 ccgaggagtt ccagtgatca agtggaagaa agatggcatt catctggcct tgggaatgga
     7621 tgaaaggaag cagcaacttt caaatgggtc tctgctgata caaaacatac ttcattccag
     7681 acaccacaag ccagatgagg gactttacca atgtgaggca tctttaggag attctggctc
     7741 aattattagt cggacagcaa aagttgcagt agcaggacca ctgaggttcc tttcacagac
     7801 agaatctgtc acagccttca tgggagacac agtgctactc aagtgtgaag tcattgggga
     7861 gcccatgcca acaatccact ggcagaagaa ccaacaagac ctgactccaa tcccaggtga
     7921 ctcccgagtg gtggtcttgc cctctggagc attgcagatc agcggactcc aaccggggga
     7981 cattggaatt taccgatgct cagctcgaaa tccagccagc tcaagaacag gaaatgaagc
     8041 agaagtcaga attttatcag atccaggact gcatagacag ctgtattttc tgcaaagacc
     8101 atccaatgta gtagccattg aaggaaaaga tgctgtcctg gaatgttgtg tttctggcta
     8161 tcctccacca agttttacct ggttacgagg cgaggaagtc atccaactca ggtctaaaaa
     8221 gtattcttta ttgggtggaa gcaacttgct tatctccaat gtgacagatg atgacagtgg
     8281 aatgtatacc tgtgttgtca catataaaaa tgagaatatt agtgcctctg cagagctcac
     8341 agtcttggtt ccgccatggt ttttaaatca tccttccaac ctgtatgcct atgaaagcat
     8401 ggatattgag tttgaatgta cagtctctgg aaagcctgtg cccactgtga attggatgaa
     8461 gaatggagat gtggtcattc ctagtgatta ttttcagata gtgggaggaa gcaacttacg
     8521 gatacttggg gtggtgaagt cagatgaagg cttttatcaa tgtgtggctg aaaatgaggc
     8581 tggaaatgcc cagaccagtg cacagctcat tgtccctaag cctgctatcc caagctccag
     8641 tgtcctccct tcggctccca gagatgtggt ccctgtcttg gtttccagcc gatttgtccg
     8701 tctcagctgg cgcccacctg cagaagcgaa agggaacatt caaactttca cggtcttttt
     8761 ctccagagaa ggtgacaaca gggaacgagc attgaataca acacagcctg ggtcccttca
     8821 gctcactgtg ggaaacctga agccagaagc catgtacacc tttcgagttg tggcttacaa
     8881 tgaatgggga ccgggagaga gttctcaacc catcaaggtg gccacacagc ctgagttgca
     8941 agttccaggg ccagtagaaa acctgcaagc tgtatctacc tcacctacct caattcttat
     9001 tacctgggaa ccccctgcct atgcaaacgg tccagtccaa ggttacagat tgttctgcac
     9061 tgaggtgtcc acaggaaaag aacagaatat agaggttgat ggactatctt ataaactgga
     9121 aggcctgaaa aaattcaccg aatatagtct tcgattctta gcttataatc gctatggtcc
     9181 gggcgtctct actgatgata taacagtggt tacactttct gacgtgccaa gtgccccgcc
     9241 tcagaacgtc tccctggaag tggtcaattc aagaagtatc aaagttagct ggctgcctcc
     9301 tccatcagga acacaaaatg gatttattac cggctataaa attcgacaca gaaagacgac
     9361 ccgcaggggt gagatggaaa cactggagcc aaacaacctc tggtacctat tcacaggact
     9421 ggagaaagga agtcagtaca gtttccaggt gtcagccatg acagtcaatg gtactggacc
     9481 accttccaac tggtatactg cagagactcc agagaatgat ctagatgaat ctcaagttcc
     9541 tgatcaacca agctctcttc atgtgaggcc ccagactaac tgcatcatca tgagttggac
     9601 tcctcccttg aacccaaaca tcgtggtgcg aggttatatt atcggttatg gcgttgggag
     9661 cccttacgct gagacagtgc gtgtggacag caagcagcga tattattcca ttgagaggtt
     9721 agagtcaagt tcccattatg taatctccct aaaagctttt aacaatgccg gagaaggagt
     9781 tcctctttat gaaagtgcca ccaccaggtc tataaccgat cccactgacc cagttgatta
     9841 ttatcctttg cttgatgatt tccccacctc ggtcccagat ctctccaccc ccatgctccc
     9901 accagtaggt gtacaggctg tggctcttac ccatgatgct gtgagggtca gctgggcaga
     9961 caactctgtc cctaagaacc aaaagacgtc tgaggtgcga ctttacaccg tccggtggag
    10021 aaccagcttt tctgcaagtg caaaatacaa gtcagaagac acaacatctc taagttacac
    10081 agcaacaggc ctcaaaccaa acacaatgta tgaattctcg gtcatggtaa caaaaaacag
    10141 aaggtccagt acttggagca tgactgcaca tgccaccacg tatgaagcag cccccacctc
    10201 tgctcccaag gacttgacag tcattactag ggaagggaag cctcgtgccg tcattgtgag
    10261 ttggcagcct cccttggaag ccaatgggaa aattactgct tacatcttat tttatacctt
    10321 ggacaagaac atcccaattg atgactggat tatggaaaca atcagtggtg ataggcttac
    10381 tcatcaaatc atggatctca accttgatac tatgtattac tttcgaattc aagcacgaaa
    10441 ttcaaaagga gtggggccac tctctgatcc tatcctcttc aggactctga aagtggaaca
    10501 ccctgacaaa atggctaatg accaaggtcg tcatggagat ggaggttatt ggccagttga
    10561 tactaatttg attgatagaa gcaccctaaa tgagccgcca attggacaaa tgcacccccc
    10621 gcatggcagt gtcactcctc agaagaacag caacctgggc gcgcc
//
