ID pET-28a-MBP-TEV-Betv1; SV 1; circular; unknown; STD; UNC; 6918 BP. DT 10-AUG.-2021 DE LMBP 11976. OS unknown OC CC Accession number: LMBP 11976 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 10-AUG-2021 CC NGS data implemented FH Key Location/Qualifiers FH FT promoter 209..250 FT /label="T7 promoter with lac operator" FT RBS 251..294 FT /label="5' UTR of T7 gene 10, incl. RBS" FT misc_feature 308..325 FT /label="His-tag" FT misc_feature 335..352 FT /label="thrombin recognition site" FT CDS 368..1477 FT /label="malE" FT misc_feature 1481..1501 FT /label="TEV recognition site" FT CDS 1505..1984 FT /label="Bet v 1" FT misc_feature 1991..2008 FT /label="His-tag" FT terminator 2075..2122 FT /label="T7 terminator" FT rep_origin 2159..2614 FT /label="f1 ori" FT CDS complement(2706..3521) FT /label="Tn903 neomycin resistance" FT rep_origin 4013..4415 FT /label="pMB1 ori" FT CDS complement(4658..4849) FT /label="rop" FT CDS complement(5658..6740) FT /label="lacI" FT promoter complement(6741..6818) FT /label="lacI promoter, incl. RBS" SQ SEQUENCE 6918 AA; 2137710 MW; CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA 60 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 120 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA 180 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG 240 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG 300 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCCATATGGC 360 TAGCGGTAAA ATCGAAGAAG GTAAACTGGT AATCTGGATT AACGGCGATA AAGGCTATAA 420 CGGTCTCGCT GAAGTCGGTA AGAAATTCGA GAAAGATACC GGAATTAAAG TCACCGTTGA 480 GCATCCGGAT AAACTGGAAG AGAAATTCCC ACAGGTTGCG GCAACTGGCG ATGGCCCTGA 540 CATTATCTTC TGGGCACACG ACCGCTTTGG TGGCTACGCT CAATCTGGCC TGTTGGCTGA 600 AATCACCCCG GACAAAGCGT TCCAGGACAA GCTGTATCCG TTTACCTGGG ATGCCGTACG 660 TTACAACGGC AAGCTGATTG CTTACCCGAT CGCTGTTGAA GCGTTATCGC TGATTTATAA 720 CAAAGATCTG CTGCCGAACC CGCCAAAAAC CTGGGAAGAG ATCCCGGCGC TGGATAAAGA 780 ACTGAAAGCG AAAGGTAAGA GCGCGCTGAT GTTCAACCTG CAAGAACCGT ACTTCACCTG 840 GCCGCTGATT GCTGCTGACG GGGGTTATGC GTTCAAGTAT GAAAACGGCA AGTACGACAT 900 TAAAGACGTG GGCGTGGATA ACGCTGGCGC GAAAGCGGGT CTGACCTTCC TGGTTGACCT 960 GATTAAAAAC AAACACATGA ATGCAGACAC CGATTACTCC ATCGCAGAAG CTGCCTTTAA 1020 TAAAGGCGAA ACAGCGATGA CCATCAACGG CCCGTGGGCA TGGTCCAACA TCGACACCAG 1080 CAAAGTGAAT TATGGTGTAA CGGTACTGCC GACCTTCAAG GGTCAACCAT CCAAACCGTT 1140 CGTTGGCGTG CTGAGCGCAG GTATTAACGC CGCCAGTCCG AACAAAGAGC TGGCGAAAGA 1200 GTTCCTCGAA AACTATCTGC TGACTGATGA AGGTCTGGAA GCGGTTAATA AAGACAAACC 1260 GCTGGGTGCC GTAGCGCTGA AGTCTTACGA GGAAGAGTTG GCGAAAGATC CACGTATTGC 1320 CGCCACCATG GAAAACGCCC AGAAAGGTGA AATCATGCCG AACATCCCGC AGATGTCCGC 1380 TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC AGACTGTCGA 1440 TGAAGCCCTG AAAGACGCGC AGACTCGTAT CACCAAGGGT GAAAATCTAT ACTTCCAAGG 1500 ATCCGGTGTG TTTAACTATG AAACCGAAAC CACCAGTGTT ATTCCGGCAG CACGTCTGTT 1560 TAAAGCATTT ATTCTGGATG GCGATAACCT GTTTCCGAAA GTTGCACCGC AGGCAATTAG 1620 CAGCGTTGAA AATATTGAAG GTAATGGTGG TCCGGGTACG ATCAAAAAAA TCAGCTTTCC 1680 GGAAGGTTTC CCGTTCAAAT ATGTTAAAGA TCGTGTGGAT GAAGTGGACC ACACCAACTT 1740 CAAATATAAC TATAGCGTGA TTGAAGGTGG TCCGATTGGT GATACCCTGG AAAAAATCTC 1800 CAACGAGATT AAAATCGTTG CAACACCGGA TGGTGGTAGC ATTCTGAAAA TTAGCAACAA 1860 GTATCACACC AAAGGCGATC ATGAAGTTAA AGCCGAACAG GTTAAAGCCA GCAAAGAAAT 1920 GGGTGAAACC CTGCTGCGTG CAGTTGAAAG CTATCTGCTG GCACATAGTG ATGCCTATAA 1980 CTAACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA 2040 AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA 2100 ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATTGG CGAATGGGAC 2160 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 2220 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 2280 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 2340 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 2400 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 2460 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 2520 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 2580 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC 2640 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAATT 2700 AATTCTTAGA AAAACTCATC GAGCATCAAA TGAAACTGCA ATTTATTCAT ATCAGGATTA 2760 TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG GAGAAAACTC ACCGAGGCAG 2820 TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC CGACTCGTCC AACATCAATA 2880 CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA GTGAGAAATC ACCATGAGTG 2940 ACGACTGAAT CCGGTGAGAA TGGCAAAAGT TTATGCATTT CTTTCCAGAC TTGTTCAACA 3000 GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCATCAA CCAAACCGTT ATTCATTCGT 3060 GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCTGTTAA AAGGACAATT ACAAACAGGA 3120 ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA CAATATTTTC ACCTGAATCA 3180 GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCGGGGA TCGCAGTGGT GAGTAACCAT 3240 GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA GAGGCATAAA TTCCGTCAGC 3300 CAGTTTAGTC TGACCATCTC ATCTGTAACA TCATTGGCAA CGCTACCTTT GCCATGTTTC 3360 AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT AGATTGTCGC ACCTGATTGC 3420 CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG CATCCATGTT GGAATTTAAT 3480 CGCGGCCTAG AGCAAGACGT TTCCCGTTGA ATATGGCTCA TAACACCCCT TGTATTACTG 3540 TTTATGTAAG CAGACAGTTT TATTGTTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 3600 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 3660 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 3720 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 3780 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 3840 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 3900 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 3960 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 4020 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 4080 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 4140 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 4200 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 4260 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 4320 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 4380 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC 4440 GCATCTGTGC GGTATTTCAC ACCGCAATGG TGCACTCTCA GTACAATCTG CTCTGATGCC 4500 GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC 4560 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT 4620 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC 4680 CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA 4740 TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC 4800 TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG 4860 TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA 4920 CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC 4980 TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG 5040 TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA 5100 ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA 5160 AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC 5220 GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG 5280 TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG 5340 CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT 5400 GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT 5460 CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA 5520 AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG 5580 GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA 5640 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 5700 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT 5760 TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG 5820 AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG 5880 GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA 5940 TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA 6000 TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT 6060 TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG 6120 CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG 6180 CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC 6240 GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA 6300 AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC 6360 GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA 6420 CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG 6480 GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG 6540 GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG 6600 TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG 6660 GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG 6720 TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT 6780 GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT 6840 ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC 6900 CGCAAGGAAT GGTGCATG 6918 //