ID pET-28a-MBP-TEV-Derf1; SV 1; circular; unknown; STD; UNC; 7350 BP. DT 10-AUG.-2021 DE LMBP 11979. OS unknown OC CC Accession number: LMBP 11979 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 10-AUG-2021 CC NGS data implemented FH Key Location/Qualifiers FH FT promoter 209..250 FT /label="T7 promoter with lac operator" FT RBS 251..294 FT /label="5' UTR of T7 gene 10, incl. RBS" FT misc_feature 308..325 FT /label="His-tag" FT misc_feature 335..352 FT /label="thrombin recognition site" FT CDS 368..1477 FT /label="malE" FT misc_feature 1481..1501 FT /label="TEV recognition site" FT CDS 1505..2416 FT /label="Der f 1" FT misc_feature 2423..2440 FT /label="His-tag" FT terminator 2507..2554 FT /label="T7 terminator" FT rep_origin 2591..3046 FT /label="f1 ori" FT CDS complement(3138..3953) FT /label="Tn903 neomycin resistance" FT rep_origin 4445..4847 FT /label="pMB1 ori" FT CDS complement(5090..5281) FT /label="rop" FT CDS complement(6090..7172) FT /label="lacI" FT promoter complement(7173..7250) FT /label="lacI promoter, incl. RBS" SQ SEQUENCE 7350 AA; 2271715 MW; CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA 60 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 120 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA 180 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG 240 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG 300 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCCATATGGC 360 TAGCGGTAAA ATCGAAGAAG GTAAACTGGT AATCTGGATT AACGGCGATA AAGGCTATAA 420 CGGTCTCGCT GAAGTCGGTA AGAAATTCGA GAAAGATACC GGAATTAAAG TCACCGTTGA 480 GCATCCGGAT AAACTGGAAG AGAAATTCCC ACAGGTTGCG GCAACTGGCG ATGGCCCTGA 540 CATTATCTTC TGGGCACACG ACCGCTTTGG TGGCTACGCT CAATCTGGCC TGTTGGCTGA 600 AATCACCCCG GACAAAGCGT TCCAGGACAA GCTGTATCCG TTTACCTGGG ATGCCGTACG 660 TTACAACGGC AAGCTGATTG CTTACCCGAT CGCTGTTGAA GCGTTATCGC TGATTTATAA 720 CAAAGATCTG CTGCCGAACC CGCCAAAAAC CTGGGAAGAG ATCCCGGCGC TGGATAAAGA 780 ACTGAAAGCG AAAGGTAAGA GCGCGCTGAT GTTCAACCTG CAAGAACCGT ACTTCACCTG 840 GCCGCTGATT GCTGCTGACG GGGGTTATGC GTTCAAGTAT GAAAACGGCA AGTACGACAT 900 TAAAGACGTG GGCGTGGATA ACGCTGGCGC GAAAGCGGGT CTGACCTTCC TGGTTGACCT 960 GATTAAAAAC AAACACATGA ATGCAGACAC CGATTACTCC ATCGCAGAAG CTGCCTTTAA 1020 TAAAGGCGAA ACAGCGATGA CCATCAACGG CCCGTGGGCA TGGTCCAACA TCGACACCAG 1080 CAAAGTGAAT TATGGTGTAA CGGTACTGCC GACCTTCAAG GGTCAACCAT CCAAACCGTT 1140 CGTTGGCGTG CTGAGCGCAG GTATTAACGC CGCCAGTCCG AACAAAGAGC TGGCGAAAGA 1200 GTTCCTCGAA AACTATCTGC TGACTGATGA AGGTCTGGAA GCGGTTAATA AAGACAAACC 1260 GCTGGGTGCC GTAGCGCTGA AGTCTTACGA GGAAGAGTTG GCGAAAGATC CACGTATTGC 1320 CGCCACCATG GAAAACGCCC AGAAAGGTGA AATCATGCCG AACATCCCGC AGATGTCCGC 1380 TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC AGACTGTCGA 1440 TGAAGCCCTG AAAGACGCGC AGACTCGTAT CACCAAGGGT GAAAATCTAT ACTTCCAAGG 1500 ATCCCGTCCG GCAAGCATTA AAACCTTTGA AGAATTCAAA AAAGCCTTCA ACAAGAACTA 1560 TGCCACCGTG GAAGAAGAAG AGGTTGCACG TAAAAACTTT CTGGAAAGCC TGAAATATGT 1620 GGAAGCAAAC AAAGGTGCCA TTAACCATCT GAGCGATCTG AGCCTGGATG AATTTAAGAA 1680 TCGTTATCTG ATGAGCGCAG AAGCATTTGA ACAGCTGAAA ACCCAGTTTG ATCTGAATGC 1740 AGAAACCAGC GCATGTCGTA TTAATAGCGT TAATGTTCCG AGCGAACTGG ATCTGCGTAG 1800 CCTGCGTACC GTTACACCGA TTCGTATGCA AGGTGGTTGT GGTAGCTGTT GGGCATTTAG 1860 CGGTGTTGCA GCAACCGAAA GCGCATATCT GGCATATCGT AATACCAGCC TGGATCTGAG 1920 TGAACAAGAG CTGGTTGATT GTGCAAGCCA GCATGGTTGT CATGGTGATA CCATTCCGCG 1980 TGGTATTGAA TATATCCAGC AGAATGGTGT TGTTGAGGAA CGTAGCTATC CGTATGTTGC 2040 ACGTGAACAG CAGTGTCGTC GTCCGAATAG CCAGCATTAT GGTATTAGCA ATTACTGCCA 2100 GATTTATCCG CCTGATGTGA AACAAATTCG TGAAGCACTG ACCCAGACAC ATACCGCAAT 2160 TGCAGTTATT ATTGGCATCA AAGATCTGCG TGCGTTTCAG CACTATGATG GTCGTACCAT 2220 TATTCAGCAT GATAATGGTT ATCAGCCGAA CTATCATGCC GTGAATATTG TTGGTTATGG 2280 TAGCACCCAG GGTGTTGATT ATTGGATTGT TCGTAATAGC TGGGATACCA CCTGGGGTGA 2340 TAGCGGTTAT GGTTATTTTC AGGCAGGTAA TAACCTGATG ATGATCGAAC AGTATCCGTA 2400 CGTGGTGATC ATGTAACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA 2460 AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT 2520 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATT 2580 GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 2640 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 2700 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 2760 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 2820 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 2880 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 2940 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 3000 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC 3060 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 3120 CGCTCATGAA TTAATTCTTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC 3180 ATATCAGGAT TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC 3240 TCACCGAGGC AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT 3300 CCAACATCAA TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA 3360 TCACCATGAG TGACGACTGA ATCCGGTGAG AATGGCAAAA GTTTATGCAT TTCTTTCCAG 3420 ACTTGTTCAA CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG 3480 TTATTCATTC GTGATTGCGC CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA 3540 TTACAAACAG GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT 3600 TCACCTGAAT CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG 3660 GTGAGTAACC ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA 3720 AATTCCGTCA GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT 3780 TTGCCATGTT TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC 3840 GCACCTGATT GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG 3900 TTGGAATTTA ATCGCGGCCT AGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC 3960 CTTGTATTAC TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGACCAAAA TCCCTTAACG 4020 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 4080 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 4140 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 4200 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 4260 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 4320 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 4380 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 4440 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 4500 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 4560 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 4620 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 4680 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 4740 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 4800 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA 4860 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCAAT GGTGCACTCT CAGTACAATC 4920 TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA 4980 TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC 5040 CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT 5100 CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA 5160 GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG 5220 TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA 5280 CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG 5340 AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG 5400 AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT 5460 GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA 5520 TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA 5580 CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT 5640 CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC 5700 AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGG GCCGCCATGC 5760 CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT 5820 GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC 5880 AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT 5940 GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA 6000 AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG AGATCCCGGT GCCTAATGAG 6060 TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 6120 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 6180 GCCAGGGTGG TTTTTCTTTT CACCAGTGAG ACGGGCAACA GCTGATTGCC CTTCACCGCC 6240 TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC 6300 TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC 6360 ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG CATTGCGCCC 6420 AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA CGATGCCCTC ATTCAGCATT 6480 TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC CTTCCCGTTC CGCTATCGGC 6540 TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA 6600 GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC 6660 ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG TGTCTGGTCA 6720 GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG CTTCCACAGC AATGGCATCC 6780 TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC GTTGCGCGAG AAGATTGTGC 6840 ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA 6900 CCCAGTTGAT CGGCGCGAGA TTTAATCGCC GCGACAATTT GCGACGGCGC GTGCAGGGCC 6960 AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC CCGCCAGTTG TTGTGCCACG 7020 CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA CTTTTTCCCG CGTTTTCGCA 7080 GAAACGTGGC TGGCCTGGTT CACCACGCGG GAAACGGTCT GATAAGAGAC ACCGGCATAC 7140 TCTGCGACAT CGTATAACGT TACTGGTTTC ACATTCACCA CCCTGAATTG ACTCTCTTCC 7200 GGGCGCTATC ATGCCATACC GCGAAAGGTT TTGCGCCATT CGATGGTGTC CGGGATCTCG 7260 ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT 7320 GAGCACCGCC GCCGCAAGGA ATGGTGCATG 7350 //