ID pGB-01-02-ccdB; SV 1; circular; 3470 BP.; STD; UNC; 3470 BP. AC unknown; DT 30-JUL-2020 DE LMBP 12025. OS synthetic DNA construct CC Accession number: LMBP 12025 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 30-JUL-2020 CC FH Key Location/Qualifiers FH FT misc_feature 1..224 FT /label="Tn7 recombination sequence" FT CDS complement(291..824) FT /label="gentamycin resistance" FT promoter 891..925 FT /label="intl1 promoter" FT promoter complement(1013..1041) FT /label="PcS promoter" FT misc_feature 1112..1145 FT /vntifkey="21" FT /label="loxP" FT misc_feature 1183..1186 FT /label="GoldenBac overhang 01" FT promoter 1237..1325 FT /label="AcNPV-PH promoter" FT misc_feature 1396..1479 FT /label="Twin-Strep-tag" FT misc_feature 1489..1512 FT /label="PreScission site" FT CDS 1638..1946 FT /vntifkey="4" FT /label="ccdB" FT misc_feature 1966..1983 FT /label="His-tag" FT terminator complement(2105..2201) FT /label="SV40 polyA late" FT terminator 2105..2201 FT /label="SV40 polyA early" FT misc_feature 2382..2385 FT /label="GoldenBac overhang 02" FT misc_feature 2393..2558 FT /label="Tn7 recombination sequence" FT rep_origin 3118..3421 FT /label="pMB1 ori" SQ Sequence 3470 BP; 915 A; 850 C; 823 G; 882 t; TGTGGGCGGA CAATAAAGTC TTAAACTGAA CAAAATAGAT CTAAACTATG ACAATAAAGT 60 CTTAAACTAG ACAGAATAGT TGTAAACTGA AATCAGTCCA GTTATGCTGT GAAAAAGCAT 120 ACTGGACTTT TGTTATGGCT AAAGCAAACT CTTCATTTTC TGAAGTGCAA ATTGCCCGTC 180 GTATTAAAGA GGGGCGTGGC CAAGGGCATG TAAAGACTAT ATTCGCGGCG TTGTGACAAT 240 TTACCGAACA ACTCCGCGGC CGGGAAGCCG ATCTCGGCTT GAACGAATTG TTAGGTGGCG 300 GTACTTGGGT CGATATCAAA GTGCATCACT TCTTCCCGTA TGCCCAACTT TGTATAGAGA 360 GCCACTGCGG GATCGTCACC GTAATCTGCT TGCACGTAGA TCACATAAGC ACCAAGCGCG 420 TTGGCCTCAT GCTTGAGGAG ATTGATGAGC GCGGTGGCAA TGCCCTGCCT CCGGTGCTCG 480 CCGGAGACTG CGAGATCATA GATATAGATC TCACTACGCG GCTGCTCAAA CTTGGGCAGA 540 ACGTAAGCCG CGAGAGCGCC AACAACCGCT TCTTGGTCGA AGGCAGCAAG CGCGATGAAT 600 GTCTTACTAC GGAGCAAGTT CCCGAGGTAA TCGGAGTCCG GCTGATGTTG GGAGTAGGTG 660 GCTACGTCTC CGAACTCACG ACCGAAAAGA TCAAGAGCAG CCCGCATGGA TTTGACTTGG 720 TCAGGGCCGA GCCTACATGT GCGAATGATG CCCATACTTG AGCCACCTAA CTTTGTTTTA 780 GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTGCGTA ACATCGTTGC TGCTCCATAA 840 CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG 900 TACAAAAAAA CAGTCATAAC AAGCCATGAA AACCGCCACT GCGCCGTTAC CACCGCTGCG 960 TTCGGTCAAG GTTCTGGACC AGTTGCGTGA GCGCATACGC TACTTGCATT ACAGTTTACG 1020 AACCGAACAG GCTTATGTCA ACTGGGTTCG TGCCTTCATC CGTTTCCACG GTGTGCGTCA 1080 CCCGGCAACC TTGGGCAGCA GCGAAGTCGC CATAACTTCG TATAGCATAC ATTATACGAA 1140 GTTATCTGTA ACTATAACGG TCCTAAGGTA GCGAAGGTCT CAACCTGTTT AAACACTAGT 1200 ATCGATTCGC GACCTACTCC GGAATATTAA TAGATCATGG AGATAATTAA AATGATAACC 1260 ATCTCGCAAA TAAATAAGTA TTTTACTGTT TTCGTAACAG TTTTGTAATA AAAAAACCTA 1320 TAAATATTCC GGATTATTCA TACCGTCCCA CCATCGGGCG ACGGATCCCG GTCCGAAACC 1380 ATGAAAGGGA GCGCTTGGAG CCACCCGCAG TTCGAAAAAG GTGGAGGTTC TGGCGGTGGA 1440 TCGGGAGGTT CAGCGTGGAG CCACCCGCAG TTCGAGAAAA CCGCGGGTCT GGAAGTTCTG 1500 TTCCAGGGGC CCATGGACTA ATGAGGTACC GGATCCGAAT TCGAGCTCCG TCGACAAGCT 1560 TGATATCGAA TTCCTGCAGC CCAAACTGCA GTTGACAACA TAAAAACTTT GTGTTATACT 1620 TGTAACGTAA GGAGGTAATG ATTCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT 1680 ATCGTCTGTT TGTGGATGTA CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA 1740 TCCCCCTGGC CAGTGCACGT CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG 1800 TGCATATCGG GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT 1860 CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA 1920 TTAACCTGAT GTTCTGGGGA ATATAAGCTT GCGGCCGCAC TCGAGCACCA CCACCACCAC 1980 CACTGACATG CGGTACCAAG CTTGTCGAGA AGTACTAGAG GATCATAATC AGCCATACCA 2040 CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC 2100 ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 2160 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 2220 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CTGATCACTG CTTGAGCCTA 2280 GAAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG 2340 CAATAACTAT CATAACCCCT AGGGTATACC CATCTAATTG GAACATGAGA CCAACCAGAT 2400 AAGTGAAATC TAGTTCCAAA CTATTTTGTC ATTTTTAATT TTCGTATTAG CTTACGACGC 2460 TACACCCAGT TCCCATCTAT TTTGTCACTC TTCCCTAAAT AATCCTTAAA AACTCCATTT 2520 CCACCCCTCC CAGTTCCCAA CTATTTTGTC CGCCCACAAC CGGTTGACTT GGGTCAACTG 2580 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 2640 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 2700 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 2760 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 2820 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 2880 ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 2940 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 3000 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 3060 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 3120 AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 3180 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 3240 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 3300 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 3360 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 3420 TGACTTGGGT CGCTCTTCCT GTGGATGCGC AGATGCCCTG CGTAAGCGGG 3470 //