LOCUS       pAT153HIL2              4385 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 1215.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 1215
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 23-OCT-2007
            
            
COMMENT     VNTNAME|pAT153HIL2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "EcoRI" "Eco130I" "BamHI" "BglI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4384 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Eco130I" "ccwwgg" 2 0 2 511 0 2097 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 376 0 1103 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 514 0 1663 0 1897 0 
COMMENT            3511 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 899 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #6=(CFSignal (CObList) "hIL2 mature sequence" 4 0 1 675 1076 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(675..1076)") 
COMMENT        #7=(CFSignal (CObList) "ampicillin" 4 0 1 3317 4177 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2374 2776 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pAT153HIL2'" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "length: 4385 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
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FEATURES             Location/Qualifiers
     misc_feature    complement(400..674)
                     /vntifkey="21"
                     /label=3'\UTR\of\hIL2
                     /note="3' UTR of the human interleukin 2 gene"
     CDS             complement(675..1076)
                     /vntifkey="4"
                     /label=hIL2\mature\sequence
                     /note="human interleukin 2 mature sequence"
     CDS             complement(3317..4177)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(2374..2776)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
BASE COUNT     1039 a      1135 c      1087 g      1124 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc cccccccccc ccccccccct tatcaaattt attaaatagt 
      421 tttactaacc aatctacata gatactatat ttaacattca acataataat aaatattttg 
      481 ggataaataa gtgaaaccat tttagagccc ctagggctta caaaaagaat cataaaagat 
      541 ccatatttat agttttaaga ttaagaataa tagttacaat aggtagcaaa ccatacattc 
      601 aacaataaat ataaaattta aatatttaaa taaatagaag gcctgatatg ttttaagtgg 
      661 gaagcactta attatcaagt cagtgttgag atgatgcttt gacaaaaggt aatccatctg 
      721 ttcagaaatt ctacaatggt tgctgtctca tcagcatatt cacacatgaa tgttgtttca 
      781 gatcccttta gttccagaac tattacgttg atattgctga ttaagtccct gggtcttaag 
      841 tgaaagtttt tgctttgagc taaatttagc acttcctcca gaggtttgag ttcttcttct 
      901 agacactgaa gatgtttcag ttctgtggcc ttcttgggca tgtaaaactt aaatgtgagc 
      961 atcctggtga gtttgggatt cttgtaatta ttaattccat tcaaaatcat ctgtaaatcc 
     1021 agcagtaaat gctccagttg tagctgtgtt ttctttgtag aacttgaagt aggtgcactg 
     1081 ttgggggggg gggggggggg gggatcctct acgccggacg catcgtggcc ggcatcaccg 
     1141 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 
     1201 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 
     1261 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 
     1321 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 
     1381 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga 
     1441 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg 
     1501 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc 
     1561 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg 
     1621 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg 
     1681 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc 
     1741 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg 
     1801 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca 
     1861 ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg 
     1921 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat 
     1981 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac 
     2041 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg 
     2101 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag 
     2161 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg 
     2221 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag 
     2281 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg 
     2341 tgtttcgtaa agtctggaaa cgcggaagtc agcgctcttc cgcttcctcg ctcactgact 
     2401 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     2461 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 
     3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     3601 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt 
     3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     3901 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca 
     3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 
     4321 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc 
     4381 aagaa 
//
