LOCUS       pPTK081_0_Empty      2676 bp    DNA     circular UNA 23-OCT-2023
DEFINITION  LMBP 12734.
COMMENT     Accession number: LMBP 12734
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     promoter        13..142
                     /label=glpT\promoter
     RBS             143..178
                     /label=synthetic\RBS
     CDS             179..895
                     /label=sfGFP
     terminator      907..950
                     /label=T1\terminator
     terminator      984..1024
                     /label=T7Te\terminator
     CDS             complement(1147..1806)
                     /label=chloramphenicol\resistance
     rep_origin      2358..2676
                     /label=pMB1\ori
ORIGIN
        1 GTCGGCGAGA CGGAAAGTGA AACGTGATTT CATGCGTCAT TTTGAACATT TTGTAAATCT
       61 TATTTAATAA TGTGTGCGGC AATTCACATT TAATTTATGA ATGTTTTCTT AACATCGCGG
      121 CAACTCAAGA AACGGCAGGT TCGGATCTTA GCTACTAGAG AAAGAGGAGA AATACTAGAT
      181 GCGTAAAGGC GAAGAGCTGT TCACTGGTGT CGTCCCTATT CTGGTGGAAC TGGATGGTGA
      241 TGTCAACGGT CATAAGTTTT CCGTGCGTGG CGAGGGTGAA GGTGACGCAA CTAATGGTAA
      301 ACTGACGCTG AAGTTCATCT GTACTACTGG TAAACTGCCG GTTCCTTGGC CGACTCTGGT
      361 AACGACGCTG ACTTATGGTG TTCAGTGCTT TGCTCGTTAT CCGGACCATA TGAAGCAGCA
      421 TGACTTCTTC AAGTCCGCCA TGCCGGAAGG CTATGTGCAG GAACGCACGA TTTCCTTTAA
      481 GGATGACGGC ACGTACAAAA CGCGTGCGGA AGTGAAATTT GAAGGCGATA CCCTGGTAAA
      541 CCGCATTGAG CTGAAAGGCA TTGACTTTAA AGAGGACGGC AATATCCTGG GCCATAAGCT
      601 GGAATACAAT TTTAACAGCC ACAATGTTTA CATCACCGCC GATAAACAAA AAAATGGCAT
      661 TAAAGCGAAT TTTAAAATTC GCCACAACGT GGAGGATGGC AGCGTGCAGC TGGCTGATCA
      721 CTACCAGCAA AACACTCCAA TCGGTGATGG TCCTGTTCTG CTGCCAGACA ATCACTATCT
      781 GAGCACGCAA AGCGTTCTGT CTAAAGATCC GAACGAGAAA CGCGATCATA TGGTTCTGCT
      841 GGAGTTCGTA ACCGCAGCGG GCATCACGCA TGGTATGGAT GAACTGTACA AATGACCAGG
      901 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG
      961 TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT
     1021 TATACGTCTC TGACCAGACC AATAAAAAAC GCCCGGCGGC AACCGAGCGT TCTGAACAAA
     1081 TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA GGAGTCCAAG CGAGCTCGAT
     1141 ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT
     1201 GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC
     1261 CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT
     1321 ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AAACGAAAAA
     1381 CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC
     1441 TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA
     1501 AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC
     1561 CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA GCATTCATCA GGCGGGCAAG
     1621 AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC
     1681 CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC
     1741 AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT
     1801 CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG
     1861 TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCCGATCA ATCATGACCA
     1921 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
     1981 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
     2041 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
     2101 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC
     2161 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
     2221 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
     2281 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
     2341 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
     2401 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
     2461 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
     2521 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
     2581 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
     2641 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTA
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