ID   pPTK010_P4a_HIS-tag; SV 1; circular; unknown; STD; UNC; 1693 BP.
DT   23-OCT-2023
DE   LMBP 12754.
CC   Accession number: LMBP 12754
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(116..775)
FT                   /label="chloramphenicol resistance"
FT   rep_origin      1327..1645
FT                   /label="pMB1 ori"
FT   misc_feature    1660..1683
FT                   /label="His-tag"
SQ   SEQUENCE  1693 AA;  522639 MW;
     GACCAGACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG        60
     TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC GAGCTCGATA TCAAATTACG       120
     CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA       180
     AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT       240
     GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT       300
     TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA AACGAAAAAC ATATTCTCAA       360
     TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA       420
     TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG       480
     TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT       540
     CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA       600
     AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA       660
     GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT       720
     TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG       780
     CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT       840
     CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCCGATCAA TCATGACCAA AATCCCTTAA       900
     CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA       960
     GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG      1020
     GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC      1080
     AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG      1140
     AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC      1200
     AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG      1260
     CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC      1320
     ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA      1380
     AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT      1440
     CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG      1500
     CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG      1560
     GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA      1620
     TCCCCTGATT CTGTGGATAA CCGTAGTCGG TCTCAATCCC ATCATCACCA CCATCATCAC      1680
     CATTAATGGC TGA                                                         1693
//