ID   pPTK066_P6_Lox71-BlastR-Lox66; SV 1; circular; unknown; STD; UNC; 3007 BP.
DT   30-NOV-2023
DE   LMBP 12777.
CC   Accession number: LMBP 12777
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 30-NOV-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(112..771)
FT                   /label="chloramphenicol resistance"
FT   rep_origin      1323..1641
FT                   /label="pMB1 ori"
FT   misc_recomb     1681..1714
FT                   /label="Lox71"
FT   promoter        1715..2271
FT                   /label="ILV5 promoter"
FT   promoter        2272..2336
FT                   /label="synthetic EM72 promoter"
FT   CDS             2337..2735
FT                   /label="blasticidin resistance"
FT   terminator      2736..2937
FT                   /label="E. gossypii TEF1 terminator"
FT   misc_recomb     2938..2971
FT                   /label="Lox66"
SQ   SEQUENCE  3007 AA;  929345 MW;
     AGACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT        60
     GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC       120
     GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC       180
     ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT       240
     ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA       300
     ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAAACG AAAAACATAT TCTCAATAAA       360
     CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG       420
     TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG       480
     CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT       540
     CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC       600
     CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG       660
     AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG       720
     ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC       780
     CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT       840
     ATGGTGAAAG TTGGAACCTC TTACGTGCCC GATCAATCAT GACCAAAATC CCTTAACGTG       900
     AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC       960
     CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG      1020
     TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG      1080
     CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT      1140
     CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG      1200
     GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC      1260
     GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG      1320
     AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG      1380
     CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG      1440
     GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC      1500
     GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT      1560
     TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC      1620
     CTGATTCTGT GGATAACCGT AGTCGGTCTC ATACAACAGG GACTAAAGAG GTGATGATTA      1680
     TACCGTTCGT ATAGCATACA TTATACGAAG TTATATCCTT CAGTAATGTC TTGTTTCTTT      1740
     TGTTGCAGTG GTGAGCCATT TTGACTTCGT GAAAGTTTCT TTAGAATAGT TGTTTCCAGA      1800
     GGCCAAACAT TCCACCCGTA GTAAAGTGCA AGCGTAGGAG AACCAAGACT GGCATAAATC      1860
     AGGTATAAGT GTCGAGCACT GGCAGGTGAT CTTCTGAAAG TTTCTACTAG CAGATAAGAT      1920
     CCAGTAGTCA TGCATATGGC AACAATGTAC CGTGTGGATC TAAGAACGCG TCCTACTAAC      1980
     CTTCGCATTC GTTGGTCCAG TTTGTTGTTA TCGATCAACG TGACAAGGTT GTCGATTCCG      2040
     CGTAAGCATG CATACCCAAG GACGCCTGTT GCAATTCCAA GTGAGCCAGT TCCAACAATC      2100
     TTTGTAATAT TAGAGCACTT CATTGTGTTG CGCTTGAAAG TAAAATGCGA ACAAATTAAG      2160
     AGATAATCTC GAAACCGCGA CTTCAAACGC CAATATGATG TGCGGCACAC AATAAGCGTT      2220
     CATATCCGCT GGGTGACTTT CTCGCTTTAA AAAATTATCC GAAAAAATTT TCTAGAGTGT      2280
     TGTTACTTTA TACTTCCGGC TCGTATAATA CGACAAGGTG TAAGGAGGAC TAAACCATGG      2340
     CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA      2400
     GCATCCCCAT CTCTGAAGAT TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA      2460
     TCTTCACTGG TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC      2520
     TGGGCACTGC TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG      2580
     AGAACAGGGG CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC      2640
     CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG      2700
     AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAAGTAA CTGACAATAA AAAGATTCTT      2760
     GTTTTCAAGA ACTTGTCATT TGTATAGTTT TTTTATATTG TAGTTGTTCT ATTTTAATCA      2820
     AATGTTAGCG TGATTTATAT TTTTTTTCGC CTCGACATCA TCTGCCCAGA TGCGAAGTTA      2880
     AGTGCGCAGA AAGTAATATC ATGCGTCAAT CGTATGTGAA TGCTGGTCGC TATACTGATA      2940
     ACTTCGTATA GCATACATTA TACGAACGGT AGACAGTATC GAATGGACTC TGATGAGAGT      3000
     TGAGACC                                                                3007
//