LOCUS       pPTK080_P8_AmpR      2773 bp    DNA     circular UNA 23-OCT-2023
DEFINITION  LMBP 12785.
COMMENT     Accession number: LMBP 12785
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     rep_origin      447..764
                     /label=pMB1\ori
     promoter        778..831
                     /label=PLtetO-1\promoter
     RBS             832..856
                     /label=synthetic\RBS
     CDS             857..1534
                     /label=mRFP1
     terminator      1555..1598
                     /label=T1\terminator
     terminator      1632..1672
                     /label=T7Te\terminator
     CDS             complement(1782..2642)
                     /label=ampicillin\resistance
ORIGIN
        1 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
       61 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
      121 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      181 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      241 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      301 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      361 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      421 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      481 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      541 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      601 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      661 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
      721 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC
      781 CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA
      841 AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA
      901 AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG
      961 TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC
     1021 GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA
     1081 CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG
     1141 TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA
     1201 CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT
     1261 TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG
     1321 TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC
     1381 TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC
     1441 CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA
     1501 ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA
     1561 CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT
     1621 CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC
     1681 TCACCGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     1741 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     1801 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     1861 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     1921 CGGGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     1981 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2041 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2101 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2161 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2221 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2281 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2341 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2401 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     2461 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     2521 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     2581 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     2641 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     2701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     2761 AAAGTGCCAC CTG
//
