LOCUS       pPTK196_P8_HygR      3547 bp    DNA     circular UNA 16-OCT-2023
DEFINITION  LMBP 12786.
COMMENT     Accession number: LMBP 12786
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified:16-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     rep_origin      447..764
                     /label=pMB1\ori
     promoter        778..831
                     /label=PLtetO-1\promoter
     RBS             832..856
                     /label=synthetic\RBS
     CDS             857..1534
                     /label=mRFP1
     terminator      1555..1598
                     /label=T1\terminator
     terminator      1632..1672
                     /label=T7Te\terminator
     promoter        1688..2243
                     /label=ILV5\promoter
     promoter        2244..2308
                     /label=synthetic\EM72\promoter
     CDS             2309..3337
                     /label=hygromycin\resistance
     terminator      3342..3547
                     /label=E.\gossypii\TEF1\terminator
ORIGIN
        1 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
       61 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
      121 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      181 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      241 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      301 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      361 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      421 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      481 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      541 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      601 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      661 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
      721 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC
      781 CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA
      841 AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA
      901 AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG
      961 TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC
     1021 GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA
     1081 CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG
     1141 TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA
     1201 CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT
     1261 TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG
     1321 TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC
     1381 TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC
     1441 CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA
     1501 ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA
     1561 CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT
     1621 CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC
     1681 TCACCGAATC CTTCGTAATG TCTTGTTTCT TTTGTTGCAG TGGTGAGCCA TTTTGACTTC
     1741 GTGAAAGTTT CTTTAGAATA GTTGTTTCCA GAGGCCAAAC ATTCCACCCG TAGTAAAGTG
     1801 CAAGCGTAGG AGAACCAAGA CTGGCATAAA TCAGGTATAA GTGTCGAGCA CTGGCAGGTG
     1861 ATCTTCTGAA AGTTTCTACT AGCAGATAAG ATCCAGTAGT CATGCATATG GCAACAATGT
     1921 ACCGTGTGGA TCTAAGAACG CGTCCTACTA ACCTTCGCAT TCGTTGGTCC AGTTTGTTGT
     1981 TATCGATCAA CGTGACAAGG TTGTCGATTC CGCGTAAGCA TGCATACCCA AGGACGCCTG
     2041 TTGCAATTCC AAGTGAGCCA GTTCCAACAA TCTTTGTAAT ATTAGAGCAC TTCATTGTGT
     2101 TGCGCTTGAA AGTAAAATGC GAACAAATTA AGAGATAATC TCGAAACCGC GACTTCAAAC
     2161 GCCAATATGA TGTGCGGCAC ACAATAAGCG TTCATATCCG CTGGGTGACT TTCTCGCTTT
     2221 AAAAAATTAT CCGAAAAAAT TTTCTAGAGT GTTGTTACTT TATACTTCCG GCTCGTATAA
     2281 TACGACAAGG TGTAAGGAGG ACTAAACCAT GGGTAAAAAG CCTGAACTCA CCGCGACGTC
     2341 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTGTCC GACCTGATGC AGCTCTCGGA
     2401 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT
     2461 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC
     2521 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTT AGCGAGAGCC TGACCTATTG
     2581 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC
     2641 TGTTCTGCAA CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC
     2701 GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT
     2761 CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT
     2821 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA
     2881 AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG
     2941 CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC
     3001 CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA
     3061 GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG
     3121 TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA
     3181 GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA CACAAATCGC
     3241 CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG ATAGTGGAAA
     3301 CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAAGCC GAGTAACTGA CTGACAATAA
     3361 AAAGATTCTT GTTTTCAAGA ACTTGTCATT TGTATAGTTT TTTTATATTG TAGTTGTTCT
     3421 ATTTTAATCA AATGTTAGCG TGATTTATAT TTTTTTTCGC CTCGACATCA TCTGCCCAGA
     3481 TGCGAAGTTA AGTGCGCAGA AAGTAATATC ATGCGTCAAT CGTATGTGAA TGCTGGTCGC
     3541 TATACTG
//
