LOCUS       pMC_MultiGene_HygR      5246 bp    DNA     circular UNA 17-OCT-2023
DEFINITION  LMBP 12796.
COMMENT     Accession number: LMBP 12796
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     promoter        5..561
                     /label=ILV5\promoter
     promoter        562..626
                     /label=synthetic\EM72\promoter
     CDS             627..1655
                     /label=hygromycin\resistance
     terminator      1660..1865
                     /label=E.\gossypii\TEF1\terminator
     CDS             complement(2108..2968)
                     /label=ampicillin\resistance
     rep_origin      3546..3863
                     /label=pMB1\ori
     misc_feature    3868..4036
                     /label=ConLS'\connector
     promoter        4041..4170
                     /label=glpT\promoter
     RBS             4171..4206
                     /label=synthetic\RBS
     CDS             4207..4923
                     /label=sfGFP
     terminator      4935..4978
                     /label=T1\terminator
     terminator      5012..5052
                     /label=T7Te\terminator
     misc_feature    5057..5246
                     /label=ConRE'\connector
ORIGIN
        1 TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT
       61 GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA
      121 AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT
      181 CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC
      241 CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA
      301 TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT
      361 GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG
      421 CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC
      481 CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA
      541 AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA
      601 CGACAAGGTG TAAGGAGGAC TAAACCATGG GTAAAAAGCC TGAACTCACC GCGACGTCTG
      661 TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTGTCCGA CCTGATGCAG CTCTCGGAGG
      721 GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA
      781 ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG
      841 CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTTAG CGAGAGCCTG ACCTATTGCA
      901 TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG
      961 TTCTGCAACC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA
     1021 GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA
     1081 TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA
     1141 GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG
     1201 TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA
     1261 TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA
     1321 ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC
     1381 GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC
     1441 TTGACCAACT CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG
     1501 GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC
     1561 GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC
     1621 GACGCCCCAG CACTCGTCCG AGGGCAAAGG AATAAGCCGA GTAACTGACT GACAATAAAA
     1681 AGATTCTTGT TTTCAAGAAC TTGTCATTTG TATAGTTTTT TTATATTGTA GTTGTTCTAT
     1741 TTTAATCAAA TGTTAGCGTG ATTTATATTT TTTTTCGCCT CGACATCATC TGCCCAGATG
     1801 CGAAGTTAAG TGCGCAGAAA GTAATATCAT GCGTCAATCG TATGTGAATG CTGGTCGCTA
     1861 TACTGGAGTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG
     1921 AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA
     1981 TCGGTGCGGG CCTCTTCGCT ATTACGCCAC CGAGATTATC AAAAAGGATC TTCACCTAGA
     2041 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
     2101 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
     2161 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
     2221 CTGGCCCCAG TGCTGCAATG ATACCGCGGG ACCCACGCTC ACCGGCTCCA GATTTATCAG
     2281 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
     2341 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
     2401 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
     2461 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
     2521 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
     2581 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
     2641 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
     2701 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
     2761 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
     2821 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
     2881 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
     2941 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
     3001 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
     3061 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGT CATGACCAAA ATCCCTTAAC
     3121 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     3181 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     3241 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     3301 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     3361 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     3421 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     3481 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     3541 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     3601 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     3661 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     3721 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     3781 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
     3841 CCCCTGATTC TGTGGATAAC CGTCCCTGAA TTCGCATCTA GACTGATGAG ACGTGGTAGA
     3901 GCCACAAACA GCCGGTACAA GCAACGATCT CCAGGACCAT CTGAATCATG CGCGGATGAC
     3961 ACGAACTCAC GACGGCGATC ACAGACATTA ACCCACAGTA CAGACACTGC GACAACGTGG
     4021 CAATTCGTCG CAATACAACG GAAAGTGAAA CGTGATTTCA TGCGTCATTT TGAACATTTT
     4081 GTAAATCTTA TTTAATAATG TGTGCGGCAA TTCACATTTA ATTTATGAAT GTTTTCTTAA
     4141 CATCGCGGCA ACTCAAGAAA CGGCAGGTTC GGATCTTAGC TACTAGAGAA AGAGGAGAAA
     4201 TACTAGATGC GTAAAGGCGA AGAGCTGTTC ACTGGTGTCG TCCCTATTCT GGTGGAACTG
     4261 GATGGTGATG TCAACGGTCA TAAGTTTTCC GTGCGTGGCG AGGGTGAAGG TGACGCAACT
     4321 AATGGTAAAC TGACGCTGAA GTTCATCTGT ACTACTGGTA AACTGCCGGT TCCTTGGCCG
     4381 ACTCTGGTAA CGACGCTGAC TTATGGTGTT CAGTGCTTTG CTCGTTATCC GGACCATATG
     4441 AAGCAGCATG ACTTCTTCAA GTCCGCCATG CCGGAAGGCT ATGTGCAGGA ACGCACGATT
     4501 TCCTTTAAGG ATGACGGCAC GTACAAAACG CGTGCGGAAG TGAAATTTGA AGGCGATACC
     4561 CTGGTAAACC GCATTGAGCT GAAAGGCATT GACTTTAAAG AGGACGGCAA TATCCTGGGC
     4621 CATAAGCTGG AATACAATTT TAACAGCCAC AATGTTTACA TCACCGCCGA TAAACAAAAA
     4681 AATGGCATTA AAGCGAATTT TAAAATTCGC CACAACGTGG AGGATGGCAG CGTGCAGCTG
     4741 GCTGATCACT ACCAGCAAAA CACTCCAATC GGTGATGGTC CTGTTCTGCT GCCAGACAAT
     4801 CACTATCTGA GCACGCAAAG CGTTCTGTCT AAAGATCCGA ACGAGAAACG CGATCATATG
     4861 GTTCTGCTGG AGTTCGTAAC CGCAGCGGGC ATCACGCATG GTATGGATGA ACTGTACAAA
     4921 TGACCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT
     4981 GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT
     5041 TCTGCGTTTA TAGCTGGAAA TCTGCTCGTC AGTGGTGCTC ACACTGACGA ATCATGTACA
     5101 GATCATACCG ATGACTGCCT GGCGACTCAC AACTAAGCAA GACAGCCGGA ACCAGCGCCG
     5161 GCGAACACCA CTGCATATAT GGCATATCAC AACAGTCCAC GTCTCAAGCA GTTACAGAGA
     5221 TGTTACGAAC CACTAGTGCA CTGCAG
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