ID pMC_MultiGene_HygR; SV 1; circular; unknown; STD; UNC; 5246 BP. DT 17-OCT.-2023 DE LMBP 12796. CC Accession number: LMBP 12796 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 17-OCT-2023 CC NGS data implemented FH Key Location/Qualifiers FH FT promoter 5..561 FT /label="ILV5 promoter" FT promoter 562..626 FT /label="synthetic EM72 promoter" FT CDS 627..1655 FT /label="hygromycin resistance" FT terminator 1660..1865 FT /label="E. gossypii TEF1 terminator" FT CDS complement(2108..2968) FT /label="ampicillin resistance" FT rep_origin 3546..3863 FT /label="pMB1 ori" FT misc_feature 3868..4036 FT /label="ConLS' connector" FT promoter 4041..4170 FT /label="glpT promoter" FT RBS 4171..4206 FT /label="synthetic RBS" FT CDS 4207..4923 FT /label="sfGFP" FT terminator 4935..4978 FT /label="T1 terminator" FT terminator 5012..5052 FT /label="T7Te terminator" FT misc_feature 5057..5246 FT /label="ConRE' connector" SQ SEQUENCE 5246 AA; 1621885 MW; TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT 60 GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA 120 AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT 180 CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC 240 CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA 300 TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT 360 GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG 420 CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC 480 CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA 540 AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA 600 CGACAAGGTG TAAGGAGGAC TAAACCATGG GTAAAAAGCC TGAACTCACC GCGACGTCTG 660 TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTGTCCGA CCTGATGCAG CTCTCGGAGG 720 GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA 780 ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG 840 CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTTAG CGAGAGCCTG ACCTATTGCA 900 TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG 960 TTCTGCAACC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA 1020 GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA 1080 TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA 1140 GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG 1200 TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA 1260 TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA 1320 ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC 1380 GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC 1440 TTGACCAACT CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG 1500 GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC 1560 GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC 1620 GACGCCCCAG CACTCGTCCG AGGGCAAAGG AATAAGCCGA GTAACTGACT GACAATAAAA 1680 AGATTCTTGT TTTCAAGAAC TTGTCATTTG TATAGTTTTT TTATATTGTA GTTGTTCTAT 1740 TTTAATCAAA TGTTAGCGTG ATTTATATTT TTTTTCGCCT CGACATCATC TGCCCAGATG 1800 CGAAGTTAAG TGCGCAGAAA GTAATATCAT GCGTCAATCG TATGTGAATG CTGGTCGCTA 1860 TACTGGAGTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 1920 AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA 1980 TCGGTGCGGG CCTCTTCGCT ATTACGCCAC CGAGATTATC AAAAAGGATC TTCACCTAGA 2040 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 2100 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 2160 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 2220 CTGGCCCCAG TGCTGCAATG ATACCGCGGG ACCCACGCTC ACCGGCTCCA GATTTATCAG 2280 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 2340 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 2400 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 2460 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 2520 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 2580 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 2640 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 2700 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 2760 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 2820 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 2880 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 2940 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 3000 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 3060 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGT CATGACCAAA ATCCCTTAAC 3120 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 3180 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 3240 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 3300 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 3360 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 3420 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 3480 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 3540 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 3600 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 3660 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 3720 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 3780 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 3840 CCCCTGATTC TGTGGATAAC CGTCCCTGAA TTCGCATCTA GACTGATGAG ACGTGGTAGA 3900 GCCACAAACA GCCGGTACAA GCAACGATCT CCAGGACCAT CTGAATCATG CGCGGATGAC 3960 ACGAACTCAC GACGGCGATC ACAGACATTA ACCCACAGTA CAGACACTGC GACAACGTGG 4020 CAATTCGTCG CAATACAACG GAAAGTGAAA CGTGATTTCA TGCGTCATTT TGAACATTTT 4080 GTAAATCTTA TTTAATAATG TGTGCGGCAA TTCACATTTA ATTTATGAAT GTTTTCTTAA 4140 CATCGCGGCA ACTCAAGAAA CGGCAGGTTC GGATCTTAGC TACTAGAGAA AGAGGAGAAA 4200 TACTAGATGC GTAAAGGCGA AGAGCTGTTC ACTGGTGTCG TCCCTATTCT GGTGGAACTG 4260 GATGGTGATG TCAACGGTCA TAAGTTTTCC GTGCGTGGCG AGGGTGAAGG TGACGCAACT 4320 AATGGTAAAC TGACGCTGAA GTTCATCTGT ACTACTGGTA AACTGCCGGT TCCTTGGCCG 4380 ACTCTGGTAA CGACGCTGAC TTATGGTGTT CAGTGCTTTG CTCGTTATCC GGACCATATG 4440 AAGCAGCATG ACTTCTTCAA GTCCGCCATG CCGGAAGGCT ATGTGCAGGA ACGCACGATT 4500 TCCTTTAAGG ATGACGGCAC GTACAAAACG CGTGCGGAAG TGAAATTTGA AGGCGATACC 4560 CTGGTAAACC GCATTGAGCT GAAAGGCATT GACTTTAAAG AGGACGGCAA TATCCTGGGC 4620 CATAAGCTGG AATACAATTT TAACAGCCAC AATGTTTACA TCACCGCCGA TAAACAAAAA 4680 AATGGCATTA AAGCGAATTT TAAAATTCGC CACAACGTGG AGGATGGCAG CGTGCAGCTG 4740 GCTGATCACT ACCAGCAAAA CACTCCAATC GGTGATGGTC CTGTTCTGCT GCCAGACAAT 4800 CACTATCTGA GCACGCAAAG CGTTCTGTCT AAAGATCCGA ACGAGAAACG CGATCATATG 4860 GTTCTGCTGG AGTTCGTAAC CGCAGCGGGC ATCACGCATG GTATGGATGA ACTGTACAAA 4920 TGACCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT 4980 GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT 5040 TCTGCGTTTA TAGCTGGAAA TCTGCTCGTC AGTGGTGCTC ACACTGACGA ATCATGTACA 5100 GATCATACCG ATGACTGCCT GGCGACTCAC AACTAAGCAA GACAGCCGGA ACCAGCGCCG 5160 GCGAACACCA CTGCATATAT GGCATATCAC AACAGTCCAC GTCTCAAGCA GTTACAGAGA 5220 TGTTACGAAC CACTAGTGCA CTGCAG 5246 //