ID pMC_Cre_KanR; SV 1; circular; unknown; STD; UNC; 6334 BP. DT 17-OCT.-2023 DE LMBP 12800. CC Accession number: LMBP 12800 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 17-OCT-2023 CC NGS data implemented FH Key Location/Qualifiers FH FT CDS complement(95..955) FT /label="ampicillin resistance" FT rep_origin 1533..1850 FT /label="pMB1 ori" FT misc_feature 1855..2044 FT /label="ConLS connector" FT promoter 2049..3046 FT /label="S. cerevisiae ENO1 promoter" FT CDS 3053..4087 FT /label="Cre" FT terminator 4088..4331 FT /label="AOX1 terminator" FT misc_feature 4336..4504 FT /label="ConR1 connector" FT promoter 4509..5065 FT /label="ILV5 promoter" FT promoter 5066..5130 FT /label="synthetic EM72 promoter" FT CDS 5131..5940 FT /label="Tn903 neomycin resistance" FT terminator 5945..6150 FT /label="E. gossypii TEF1 terminator" FT rep_origin 6161..6324 FT /label="Pichia ARS1" SQ SEQUENCE 6334 AA; 1957088 MW; GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 60 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 120 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 180 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGGGACC 240 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 300 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 360 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 420 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 480 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 540 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 600 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 660 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 720 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 780 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 840 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 900 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 960 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 1020 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 1080 CACCTGTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 1140 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 1200 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 1260 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT 1320 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 1380 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 1440 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 1500 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 1560 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 1620 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 1680 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 1740 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 1800 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT CCCTGAATTC 1860 GCATCTAGAT GGTAGAGCCA CAAACAGCCG GTACAAGCAA CGATCTCCAG GACCATCTGA 1920 ATCATGCGCG GATGACACGA ACTCACGACG GCGATCACAG ACATTAACCC ACAGTACAGA 1980 CACTGCGACA ACGTGGCAAT TCGTCGCAAT ACCGTCTCAC TGAACTGGCC GATAATTGCA 2040 GACGAACGAG AAAGCATACT ATACTATTCG ACATTCCTTT CAATCCTGGA ATTAACAGTC 2100 ACTTTTAAAA AAGACATCTA CCGTGAAGGT GCCGTAGAGT ATCGCGTTAC CATATCGCCA 2160 AAAACTGATA TACGCCGCGG AAACCAGGCA AACAATTGAA AAGAAAAATT TTGAGGAACT 2220 CTCTGCATCG AAGCCGTCTA GAGTTACCAC TAGTCAGATG CCGCGGGCAC TTGAGCACCT 2280 CATGCACAGC AATAACACAA CACAATGGTT AGTAGCAACC TGAATTCGGT CATTGATGCA 2340 TGCATGTGCC GTGAAGCGGG ACAACCAGAA AAGTCGTCTA TAAATGCCGG CACGTGCGAT 2400 CATCGTGGCG GGGTTTTAAG AGTGCATATC ACAAATTGTC GCATTACCGC GGAACCGCCA 2460 GATATTCATT ACTTGACGCA AAAGCGTTTG AAATAATGAC GAAAAAGAAG GAAGAAAAAA 2520 AAAGAAAAAT ACCGCTTCTA GGCGGGTTAT CTACTGATCC GAGCTTCCAC TAGGATAGCA 2580 CCCAAACACC TGCATATTTG GACGACCTTT ACTTACACCA CCAAAAACCA CTTTCGCCTC 2640 TCCCGCCCCT GATAACGTCC ACTAATTGAG CGATTACCTG AGCGGTCCTC TTTTGTTTGC 2700 AGCATGAGAC TTGCATACTG CAAATCGTAA GTAGCAACCT CTCAAGGTCA AAACTGTATG 2760 GAAACCTTGT CACCTCACTT AATTCTAGCT AGCCTACCCT GCAAGTCAAG AGCTCTCCGT 2820 GATTCCTAGC CACCTCAAGG TATGCCTCTC CCCGGAAACT GTGGCCTTTT CTGGCACACA 2880 TGATCTCCAC GATTTCAACA TATAAATAGC TTTTGATAAT GGCAATATTA ATCAAATTTA 2940 TTTTACTTCT TTCTTGTAAC ATCTCTCTTG TAATCCCTTA TTCCTTCTAG CTATTTTTCA 3000 TAAAAAACCA AGCAACTGCT TATCAACACA CAAACACTAA ATCAAAAGAT CTATGTCCAA 3060 TTTACTGACC GTACACCAAA ATTTGCCTGC ATTACCGGTC GATGCAACGA GTGATGAGGT 3120 TCGCAAGAAC CTGATGGACA TGTTCAGGGA TCGCCAGGCG TTTTCTGAGC ATACCTGGAA 3180 AATGCTTCTG TCCGTTTGCC GGTCGTGGGC GGCATGGTGC AAGTTGAATA ACCGGAAATG 3240 GTTTCCCGCA GAACCTGAAG ATGTTCGCGA TTATCTTCTA TATCTTCAGG CGCGCGGTCT 3300 GGCAGTAAAA ACTATCCAGC AACATTTGGG CCAGCTAAAC ATGCTTCATC GTCGGTCCGG 3360 GCTGCCACGA CCAAGTGACA GCAATGCTGT TTCACTGGTT ATGCGGCGGA TCCGAAAAGA 3420 AAACGTTGAT GCCGGTGAAC GTGCAAAACA GGCTCTAGCG TTCGAACGCA CTGATTTCGA 3480 CCAGGTTCGT TCACTCATGG AAAATAGCGA TCGCTGCCAG GATATACGTA ATCTGGCATT 3540 TCTGGGGATT GCTTATAACA CCCTGTTACG TATAGCCGAA ATTGCCAGGA TCAGGGTTAA 3600 AGATATCTCA CGTACTGACG GTGGGAGAAT GTTAATCCAT ATTGGCAGAA CGAAAACGCT 3660 GGTTAGCACC GCAGGTGTAG AGAAGGCACT TAGCCTGGGG GTAACTAAAC TGGTCGAGCG 3720 ATGGATTTCC GTGTCTGGTG TAGCTGATGA TCCGAATAAC TACCTGTTTT GCCGGGTCAG 3780 AAAAAATGGT GTTGCCGCGC CATCTGCCAC CAGCCAGCTA TCAACTCGCG CCCTGGAAGG 3840 GATTTTTGAA GCAACTCATC GATTGATTTA CGGCGCTAAG GATGACTCTG GTCAGAGATA 3900 CCTGGCCTGG TCTGGACACA GTGCCCGTGT CGGAGCCGCG CGAGATATGG CCCGCGCTGG 3960 AGTTTCAATA CCGGAGATCA TGCAAGCTGG TGGCTGGACC AATGTAAATA TTGTCATGAA 4020 CTATATCCGT AACCTGGATA GTGAAACAGG GGCAATGGTG CGCCTGCTGG AAGATGGCGG 4080 ATCCTAATCA AGAGGATGTC AGAATGCCAT TTGCCTGAGA GATGCAGGCT TCATTTTTGA 4140 TACTTTTTTA TTTGTAACCT ATATAGTATA GGATTTTTTT TGTCATTTTG TTTCTTCTCG 4200 TACGAGCTTG CTCCTGATCA GCCTATCTCG CAGCTGATGA ATATCTTGTG GTAGGGGTTT 4260 GGGAAAATCA TTCGAGTTTG ATGTTTTTCT TGGTATTTCC CACTCCTCTT CAGAGTACAG 4320 AAGATTAAGT GGCTGCCAAT GAGACGACGG GGTCATCACG GCTCATCATG CGCCAAACAA 4380 ATGTGTGCAA TACACGCTCG GATGACTGCA TGATGACCGC ACTGACTGGG GACAGCAGAT 4440 CCACCTAAGC CTGTGAGAGA AGCAGACACC CGACAGATCA AGGCAGTTAA CTAGTGCACT 4500 GCAGTACAAT CCTTCAGTAA TGTCTTGTTT CTTTTGTTGC AGTGGTGAGC CATTTTGACT 4560 TCGTGAAAGT TTCTTTAGAA TAGTTGTTTC CAGAGGCCAA ACATTCCACC CGTAGTAAAG 4620 TGCAAGCGTA GGAGAACCAA GACTGGCATA AATCAGGTAT AAGTGTCGAG CACTGGCAGG 4680 TGATCTTCTG AAAGTTTCTA CTAGCAGATA AGATCCAGTA GTCATGCATA TGGCAACAAT 4740 GTACCGTGTG GATCTAAGAA CGCGTCCTAC TAACCTTCGC ATTCGTTGGT CCAGTTTGTT 4800 GTTATCGATC AACGTGACAA GGTTGTCGAT TCCGCGTAAG CATGCATACC CAAGGACGCC 4860 TGTTGCAATT CCAAGTGAGC CAGTTCCAAC AATCTTTGTA ATATTAGAGC ACTTCATTGT 4920 GTTGCGCTTG AAAGTAAAAT GCGAACAAAT TAAGAGATAA TCTCGAAACC GCGACTTCAA 4980 ACGCCAATAT GATGTGCGGC ACACAATAAG CGTTCATATC CGCTGGGTGA CTTTCTCGCT 5040 TTAAAAAATT ATCCGAAAAA ATTTTCTAGA GTGTTGTTAC TTTATACTTC CGGCTCGTAT 5100 AATACGACAA GGTGTAAGGA GGACTAAACC ATGGGTAAGG AAAAGACTCA CGTTTCGAGG 5160 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT 5220 GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG 5280 TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA 5340 AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT 5400 GATGCATGGT TACTCACCAC TGCGATCCCC GGCAAAACAG CATTCCAGGT ATTAGAAGAA 5460 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT 5520 TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGCCT GGCTCAGGCG 5580 CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC 5640 TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAGCTTT TGCCATTCTC ACCGGATTCA 5700 GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA 5760 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA 5820 TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT 5880 ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGACGA GTTCTTCTAA 5940 GCCGAGTAAC TGACTGACAA TAAAAAGATT CTTGTTTTCA AGAACTTGTC ATTTGTATAG 6000 TTTTTTTATA TTGTAGTTGT TCTATTTTAA TCAAATGTTA GCGTGATTTA TATTTTTTTT 6060 CGCCTCGACA TCATCTGCCC AGATGCGAAG TTAAGTGCGC AGAAAGTAAT ATCATGCGTC 6120 AATCGTATGT GAATGCTGGT CGCTATACTG GAGTATGCAT TCGAGATAAG CTGGGGGAAC 6180 ATTCGCGAAA ATGAAACAAG TCGGCTGTTA TAGTATATTT ATTATAATAT TGAAAGATCT 6240 CAAAAGACTA CTTATTTTTG AATGAACCAA GTATGAAATC AACCTATTTG GGGTTGACCA 6300 AAATAAGTAA ATATTAATTG TCGAATGCAT CCGA 6334 //