LOCUS       pMC_Cre_HygR      6552 bp    DNA     circular UNA 09-OCT-2023
DEFINITION  LMBP 12801.
COMMENT     Accession number: LMBP 12801
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 9-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     CDS             complement(95..955)
                     /label=ampicillin\resistance
     rep_origin      1533..1850
                     /label=pMB1\ori
     misc_feature    1855..2043
                     /label=ConLS\connector
     promoter        join(2048..3045)
                     /label=S.\cerevisiae\ENO1\promoter
     CDS             3052..4086
                     /label=Cre
     terminator      4087..4330
                     /label=AOX1\terminator
     misc_feature    4335..4503
                     /label=ConR1\connector
     promoter        4508..5064
                     /label=ILV5\promoter
     promoter        5065..5129
                     /label=synthetic\EM72\promoter
     CDS             5130..6158
                     /label=hygromycin\resistance
     terminator      6171..6368
                     /label=E.\gossypii\TEF1\terminator
     rep_origin      6379..6542
                     /label=Pichia\ARS1
ORIGIN
        1 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
       61 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
      121 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
      181 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGGGACC
      241 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
      301 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
      361 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
      421 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
      481 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
      541 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
      601 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
      661 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
      721 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
      781 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
      841 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
      901 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
      961 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     1021 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     1081 CACCTGTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG
     1141 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC
     1201 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC
     1261 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT
     1321 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC
     1381 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT
     1441 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC
     1501 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG
     1561 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG
     1621 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG
     1681 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA
     1741 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT
     1801 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT CCCTAATTCG
     1861 CATCTAGATG GTAGAGCCAC AAACAGCCGG TACAAGCAAC GATCTCCAGG ACCATCTGAA
     1921 TCATGCGCGG ATGACACGAA CTCACGACGG CGATCACAGA CATTAACCCA CAGTACAGAC
     1981 ACTGCGACAA CGTGGCAATT CGTCGCAATA CCGTCTCACT GAACTGGCCG ATAATTGCAG
     2041 ACGAACGAGA AAGCATACTA TACTATTCGA CATTCCTTTC AATCCTGGAA TTAACAGTCA
     2101 CTTTTAAAAA AGACATCTAC CGTGAAGGTG CCGTAGAGTA TCGCGTTACC ATATCGCCAA
     2161 AAACTGATAT ACGCCGCGGA AACCAGGCAA ACAATTGAAA AGAAAAATTT TGAGGAACTC
     2221 TCTGCATCGA AGCCGTCTAG AGTTACCACT AGTCAGATGC CGCGGGCACT TGAGCACCTC
     2281 ATGCACAGCA ATAACACAAC ACAATGGTTA GTAGCAACCT GAATTCGGTC ATTGATGCAT
     2341 GCATGTGCCG TGAAGCGGGA CAACCAGAAA AGTCGTCTAT AAATGCCGGC ACGTGCGATC
     2401 ATCGTGGCGG GGTTTTAAGA GTGCATATCA CAAATTGTCG CATTACCGCG GAACCGCCAG
     2461 ATATTCATTA CTTGACGCAA AAGCGTTTGA AATAATGACG AAAAAGAAGG AAGAAAAAAA
     2521 AAGAAAAATA CCGCTTCTAG GCGGGTTATC TACTGATCCG AGCTTCCACT AGGATAGCAC
     2581 CCAAACACCT GCATATTTGG ACGACCTTTA CTTACACCAC CAAAAACCAC TTTCGCCTCT
     2641 CCCGCCCCTG ATAACGTCCA CTAATTGAGC GATTACCTGA GCGGTCCTCT TTTGTTTGCA
     2701 GCATGAGACT TGCATACTGC AAATCGTAAG TAGCAACCTC TCAAGGTCAA AACTGTATGG
     2761 AAACCTTGTC ACCTCACTTA ATTCTAGCTA GCCTACCCTG CAAGTCAAGA GCTCTCCGTG
     2821 ATTCCTAGCC ACCTCAAGGT ATGCCTCTCC CCGGAAACTG TGGCCTTTTC TGGCACACAT
     2881 GATCTCCACG ATTTCAACAT ATAAATAGCT TTTGATAATG GCAATATTAA TCAAATTTAT
     2941 TTTACTTCTT TCTTGTAACA TCTCTCTTGT AATCCCTTAT TCCTTCTAGC TATTTTTCAT
     3001 AAAAAACCAA GCAACTGCTT ATCAACACAC AAACACTAAA TCAAAAGATC TATGTCCAAT
     3061 TTACTGACCG TACACCAAAA TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT
     3121 CGCAAGAACC TGATGGACAT GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA
     3181 ATGCTTCTGT CCGTTTGCCG GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG
     3241 TTTCCCGCAG AACCTGAAGA TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG
     3301 GCAGTAAAAA CTATCCAGCA ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG
     3361 CTGCCACGAC CAAGTGACAG CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCGAAAAGAA
     3421 AACGTTGATG CCGGTGAACG TGCAAAACAG GCTCTAGCGT TCGAACGCAC TGATTTCGAC
     3481 CAGGTTCGTT CACTCATGGA AAATAGCGAT CGCTGCCAGG ATATACGTAA TCTGGCATTT
     3541 CTGGGGATTG CTTATAACAC CCTGTTACGT ATAGCCGAAA TTGCCAGGAT CAGGGTTAAA
     3601 GATATCTCAC GTACTGACGG TGGGAGAATG TTAATCCATA TTGGCAGAAC GAAAACGCTG
     3661 GTTAGCACCG CAGGTGTAGA GAAGGCACTT AGCCTGGGGG TAACTAAACT GGTCGAGCGA
     3721 TGGATTTCCG TGTCTGGTGT AGCTGATGAT CCGAATAACT ACCTGTTTTG CCGGGTCAGA
     3781 AAAAATGGTG TTGCCGCGCC ATCTGCCACC AGCCAGCTAT CAACTCGCGC CCTGGAAGGG
     3841 ATTTTTGAAG CAACTCATCG ATTGATTTAC GGCGCTAAGG ATGACTCTGG TCAGAGATAC
     3901 CTGGCCTGGT CTGGACACAG TGCCCGTGTC GGAGCCGCGC GAGATATGGC CCGCGCTGGA
     3961 GTTTCAATAC CGGAGATCAT GCAAGCTGGT GGCTGGACCA ATGTAAATAT TGTCATGAAC
     4021 TATATCCGTA ACCTGGATAG TGAAACAGGG GCAATGGTGC GCCTGCTGGA AGATGGCGGA
     4081 TCCTAATCAA GAGGATGTCA GAATGCCATT TGCCTGAGAG ATGCAGGCTT CATTTTTGAT
     4141 ACTTTTTTAT TTGTAACCTA TATAGTATAG GATTTTTTTT GTCATTTTGT TTCTTCTCGT
     4201 ACGAGCTTGC TCCTGATCAG CCTATCTCGC AGCTGATGAA TATCTTGTGG TAGGGGTTTG
     4261 GGAAAATCAT TCGAGTTTGA TGTTTTTCTT GGTATTTCCC ACTCCTCTTC AGAGTACAGA
     4321 AGATTAAGTG GCTGCCAATG AGACGACGGG GTCATCACGG CTCATCATGC GCCAAACAAA
     4381 TGTGTGCAAT ACACGCTCGG ATGACTGCAT GATGACCGCA CTGACTGGGG ACAGCAGATC
     4441 CACCTAAGCC TGTGAGAGAA GCAGACACCC GACAGATCAA GGCAGTTAAC TAGTGCACTG
     4501 CAGTACAATC CTTCAGTAAT GTCTTGTTTC TTTTGTTGCA GTGGTGAGCC ATTTTGACTT
     4561 CGTGAAAGTT TCTTTAGAAT AGTTGTTTCC AGAGGCCAAA CATTCCACCC GTAGTAAAGT
     4621 GCAAGCGTAG GAGAACCAAG ACTGGCATAA ATCAGGTATA AGTGTCGAGC ACTGGCAGGT
     4681 GATCTTCTGA AAGTTTCTAC TAGCAGATAA GATCCAGTAG TCATGCATAT GGCAACAATG
     4741 TACCGTGTGG ATCTAAGAAC GCGTCCTACT AACCTTCGCA TTCGTTGGTC CAGTTTGTTG
     4801 TTATCGATCA ACGTGACAAG GTTGTCGATT CCGCGTAAGC ATGCATACCC AAGGACGCCT
     4861 GTTGCAATTC CAAGTGAGCC AGTTCCAACA ATCTTTGTAA TATTAGAGCA CTTCATTGTG
     4921 TTGCGCTTGA AAGTAAAATG CGAACAAATT AAGAGATAAT CTCGAAACCG CGACTTCAAA
     4981 CGCCAATATG ATGTGCGGCA CACAATAAGC GTTCATATCC GCTGGGTGAC TTTCTCGCTT
     5041 TAAAAAATTA TCCGAAAAAA TTTTCTAGAG TGTTGTTACT TTATACTTCC GGCTCGTATA
     5101 ATACGACAAG GTGTAAGGAG GACTAAACCA TGGGTAAAAA GCCTGAACTC ACCGCGACGT
     5161 CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTGTC CGACCTGATG CAGCTCTCGG
     5221 AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT GTCCTGCGGG
     5281 TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC TTTGCATCGG
     5341 CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT TAGCGAGAGC CTGACCTATT
     5401 GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC GAACTGCCCG
     5461 CTGTTCTGCA ACCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT CTTAGCCAGA
     5521 CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA TGGCGTGATT
     5581 TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG GACGACACCG
     5641 TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG GACTGCCCCG
     5701 AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG GACAATGGCC
     5761 GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA TACGAGGTCG
     5821 CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG CGCTACTTCG
     5881 AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG
     5941 GTCTTGACCA ACTCTATCAG AGCTTGGTTG ACGGCAATTT CGATGATGCA GCTTGGGCGC
     6001 AGGGTCGATG CGACGCAATC GTCCGATCCG GAGCCGGGAC TGTCGGGCGT ACACAAATCG
     6061 CCCGCAGAAG CGCGGCCGTC TGGACCGATG GCTGTGTAGA AGTACTCGCC GATAGTGGAA
     6121 ACCGACGCCC CAGCACTCGT CCGAGGGCAA AGGAATAAGC CGAGTAACTG ACTGACAATA
     6181 AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT TTTTTATATT GTAGTTGTTC
     6241 TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG CCTCGACATC ATCTGCCCAG
     6301 ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA TCGTATGTGA ATGCTGGTCG
     6361 CTATACTGGA GTATGCATTC GAGATAAGCT GGGGGAACAT TCGCGAAAAT GAAACAAGTC
     6421 GGCTGTTATA GTATATTTAT TATAATATTG AAAGATCTCA AAAGACTACT TATTTTTGAA
     6481 TGAACCAAGT ATGAAATCAA CCTATTTGGG GTTGACCAAA ATAAGTAAAT ATTAATTGTC
     6541 GAATGCATCC GA
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