ID pMC_Cre_HygR; SV 1; circular; unknown; STD; UNC; 6552 BP. DT 09-OCT-2023 DE LMBP 1280. CC Accession number: LMBP 12801 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 9-OCT-2023 CC NGS data implemented FH Key Location/Qualifiers FH FT CDS complement(95..955) FT /label="ampicillin resistance" FT rep_origin 1533..1850 FT /label="pMB1 ori" FT misc_feature 1855..2043 FT /label="ConLS connector" FT promoter join(2048..3045) FT /label="S. cerevisiae ENO1 promoter" FT CDS 3052..4086 FT /label="Cre" FT terminator 4087..4330 FT /label="AOX1 terminator" FT misc_feature 4335..4503 FT /label="ConR1 connector" FT promoter 4508..5064 FT /label="ILV5 promoter" FT promoter 5065..5129 FT /label="synthetic EM72 promoter" FT CDS 5130..6158 FT /label="hygromycin resistance" FT terminator 6171..6368 FT /label="E. gossypii TEF1 terminator" FT rep_origin 6379..6542 FT /label="Pichia ARS1" SQ SEQUENCE 6552 AA; 2024585 MW; GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 60 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 120 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 180 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGGGACC 240 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 300 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 360 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 420 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 480 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 540 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 600 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 660 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 720 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 780 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 840 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 900 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 960 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 1020 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 1080 CACCTGTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 1140 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 1200 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 1260 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT 1320 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 1380 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 1440 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 1500 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 1560 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 1620 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 1680 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 1740 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 1800 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT CCCTAATTCG 1860 CATCTAGATG GTAGAGCCAC AAACAGCCGG TACAAGCAAC GATCTCCAGG ACCATCTGAA 1920 TCATGCGCGG ATGACACGAA CTCACGACGG CGATCACAGA CATTAACCCA CAGTACAGAC 1980 ACTGCGACAA CGTGGCAATT CGTCGCAATA CCGTCTCACT GAACTGGCCG ATAATTGCAG 2040 ACGAACGAGA AAGCATACTA TACTATTCGA CATTCCTTTC AATCCTGGAA TTAACAGTCA 2100 CTTTTAAAAA AGACATCTAC CGTGAAGGTG CCGTAGAGTA TCGCGTTACC ATATCGCCAA 2160 AAACTGATAT ACGCCGCGGA AACCAGGCAA ACAATTGAAA AGAAAAATTT TGAGGAACTC 2220 TCTGCATCGA AGCCGTCTAG AGTTACCACT AGTCAGATGC CGCGGGCACT TGAGCACCTC 2280 ATGCACAGCA ATAACACAAC ACAATGGTTA GTAGCAACCT GAATTCGGTC ATTGATGCAT 2340 GCATGTGCCG TGAAGCGGGA CAACCAGAAA AGTCGTCTAT AAATGCCGGC ACGTGCGATC 2400 ATCGTGGCGG GGTTTTAAGA GTGCATATCA CAAATTGTCG CATTACCGCG GAACCGCCAG 2460 ATATTCATTA CTTGACGCAA AAGCGTTTGA AATAATGACG AAAAAGAAGG AAGAAAAAAA 2520 AAGAAAAATA CCGCTTCTAG GCGGGTTATC TACTGATCCG AGCTTCCACT AGGATAGCAC 2580 CCAAACACCT GCATATTTGG ACGACCTTTA CTTACACCAC CAAAAACCAC TTTCGCCTCT 2640 CCCGCCCCTG ATAACGTCCA CTAATTGAGC GATTACCTGA GCGGTCCTCT TTTGTTTGCA 2700 GCATGAGACT TGCATACTGC AAATCGTAAG TAGCAACCTC TCAAGGTCAA AACTGTATGG 2760 AAACCTTGTC ACCTCACTTA ATTCTAGCTA GCCTACCCTG CAAGTCAAGA GCTCTCCGTG 2820 ATTCCTAGCC ACCTCAAGGT ATGCCTCTCC CCGGAAACTG TGGCCTTTTC TGGCACACAT 2880 GATCTCCACG ATTTCAACAT ATAAATAGCT TTTGATAATG GCAATATTAA TCAAATTTAT 2940 TTTACTTCTT TCTTGTAACA TCTCTCTTGT AATCCCTTAT TCCTTCTAGC TATTTTTCAT 3000 AAAAAACCAA GCAACTGCTT ATCAACACAC AAACACTAAA TCAAAAGATC TATGTCCAAT 3060 TTACTGACCG TACACCAAAA TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT 3120 CGCAAGAACC TGATGGACAT GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA 3180 ATGCTTCTGT CCGTTTGCCG GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG 3240 TTTCCCGCAG AACCTGAAGA TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG 3300 GCAGTAAAAA CTATCCAGCA ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG 3360 CTGCCACGAC CAAGTGACAG CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCGAAAAGAA 3420 AACGTTGATG CCGGTGAACG TGCAAAACAG GCTCTAGCGT TCGAACGCAC TGATTTCGAC 3480 CAGGTTCGTT CACTCATGGA AAATAGCGAT CGCTGCCAGG ATATACGTAA TCTGGCATTT 3540 CTGGGGATTG CTTATAACAC CCTGTTACGT ATAGCCGAAA TTGCCAGGAT CAGGGTTAAA 3600 GATATCTCAC GTACTGACGG TGGGAGAATG TTAATCCATA TTGGCAGAAC GAAAACGCTG 3660 GTTAGCACCG CAGGTGTAGA GAAGGCACTT AGCCTGGGGG TAACTAAACT GGTCGAGCGA 3720 TGGATTTCCG TGTCTGGTGT AGCTGATGAT CCGAATAACT ACCTGTTTTG CCGGGTCAGA 3780 AAAAATGGTG TTGCCGCGCC ATCTGCCACC AGCCAGCTAT CAACTCGCGC CCTGGAAGGG 3840 ATTTTTGAAG CAACTCATCG ATTGATTTAC GGCGCTAAGG ATGACTCTGG TCAGAGATAC 3900 CTGGCCTGGT CTGGACACAG TGCCCGTGTC GGAGCCGCGC GAGATATGGC CCGCGCTGGA 3960 GTTTCAATAC CGGAGATCAT GCAAGCTGGT GGCTGGACCA ATGTAAATAT TGTCATGAAC 4020 TATATCCGTA ACCTGGATAG TGAAACAGGG GCAATGGTGC GCCTGCTGGA AGATGGCGGA 4080 TCCTAATCAA GAGGATGTCA GAATGCCATT TGCCTGAGAG ATGCAGGCTT CATTTTTGAT 4140 ACTTTTTTAT TTGTAACCTA TATAGTATAG GATTTTTTTT GTCATTTTGT TTCTTCTCGT 4200 ACGAGCTTGC TCCTGATCAG CCTATCTCGC AGCTGATGAA TATCTTGTGG TAGGGGTTTG 4260 GGAAAATCAT TCGAGTTTGA TGTTTTTCTT GGTATTTCCC ACTCCTCTTC AGAGTACAGA 4320 AGATTAAGTG GCTGCCAATG AGACGACGGG GTCATCACGG CTCATCATGC GCCAAACAAA 4380 TGTGTGCAAT ACACGCTCGG ATGACTGCAT GATGACCGCA CTGACTGGGG ACAGCAGATC 4440 CACCTAAGCC TGTGAGAGAA GCAGACACCC GACAGATCAA GGCAGTTAAC TAGTGCACTG 4500 CAGTACAATC CTTCAGTAAT GTCTTGTTTC TTTTGTTGCA GTGGTGAGCC ATTTTGACTT 4560 CGTGAAAGTT TCTTTAGAAT AGTTGTTTCC AGAGGCCAAA CATTCCACCC GTAGTAAAGT 4620 GCAAGCGTAG GAGAACCAAG ACTGGCATAA ATCAGGTATA AGTGTCGAGC ACTGGCAGGT 4680 GATCTTCTGA AAGTTTCTAC TAGCAGATAA GATCCAGTAG TCATGCATAT GGCAACAATG 4740 TACCGTGTGG ATCTAAGAAC GCGTCCTACT AACCTTCGCA TTCGTTGGTC CAGTTTGTTG 4800 TTATCGATCA ACGTGACAAG GTTGTCGATT CCGCGTAAGC ATGCATACCC AAGGACGCCT 4860 GTTGCAATTC CAAGTGAGCC AGTTCCAACA ATCTTTGTAA TATTAGAGCA CTTCATTGTG 4920 TTGCGCTTGA AAGTAAAATG CGAACAAATT AAGAGATAAT CTCGAAACCG CGACTTCAAA 4980 CGCCAATATG ATGTGCGGCA CACAATAAGC GTTCATATCC GCTGGGTGAC TTTCTCGCTT 5040 TAAAAAATTA TCCGAAAAAA TTTTCTAGAG TGTTGTTACT TTATACTTCC GGCTCGTATA 5100 ATACGACAAG GTGTAAGGAG GACTAAACCA TGGGTAAAAA GCCTGAACTC ACCGCGACGT 5160 CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTGTC CGACCTGATG CAGCTCTCGG 5220 AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT GTCCTGCGGG 5280 TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC TTTGCATCGG 5340 CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT TAGCGAGAGC CTGACCTATT 5400 GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC GAACTGCCCG 5460 CTGTTCTGCA ACCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT CTTAGCCAGA 5520 CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA TGGCGTGATT 5580 TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG GACGACACCG 5640 TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG GACTGCCCCG 5700 AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG GACAATGGCC 5760 GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA TACGAGGTCG 5820 CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG CGCTACTTCG 5880 AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG 5940 GTCTTGACCA ACTCTATCAG AGCTTGGTTG ACGGCAATTT CGATGATGCA GCTTGGGCGC 6000 AGGGTCGATG CGACGCAATC GTCCGATCCG GAGCCGGGAC TGTCGGGCGT ACACAAATCG 6060 CCCGCAGAAG CGCGGCCGTC TGGACCGATG GCTGTGTAGA AGTACTCGCC GATAGTGGAA 6120 ACCGACGCCC CAGCACTCGT CCGAGGGCAA AGGAATAAGC CGAGTAACTG ACTGACAATA 6180 AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT TTTTTATATT GTAGTTGTTC 6240 TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG CCTCGACATC ATCTGCCCAG 6300 ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA TCGTATGTGA ATGCTGGTCG 6360 CTATACTGGA GTATGCATTC GAGATAAGCT GGGGGAACAT TCGCGAAAAT GAAACAAGTC 6420 GGCTGTTATA GTATATTTAT TATAATATTG AAAGATCTCA AAAGACTACT TATTTTTGAA 6480 TGAACCAAGT ATGAAATCAA CCTATTTGGG GTTGACCAAA ATAAGTAAAT ATTAATTGTC 6540 GAATGCATCC GA 6552 //