LOCUS       pMC_Cre_ZeoR      5895 bp    DNA     circular UNA 17-OCT-2023
DEFINITION  LMBP 12802.
COMMENT     Accession number: LMBP 12802
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     CDS             complement(95..955)
                     /label=ampicillin\resistance
     rep_origin      1533..1850
                     /label=pMB1\ori
     misc_feature    1855..2044
                     /label=ConLS\connector
     promoter        2049..3046
                     /label=S.\cerevisiae\ENO1\promoter
     CDS             3053..4087
                     /label=Cre
     terminator      4088..4331
                     /label=AOX1\terminator
     misc_feature    4336..4504
                     /label=ConR1\connector
     promoter        4509..5065
                     /label=ILV5\promoter
     promoter        5066..5130
                     /label=synthetic\EM72\promoter
     CDS             5131..5505
                     /label=zeocin\resistance
     terminator      5510..5711
                     /label=E.\gossypii\TEF1\terminator
     rep_origin      5722..5885
                     /label=Pichia\ARS1
ORIGIN
        1 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
       61 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
      121 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
      181 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGGGACC
      241 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
      301 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
      361 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
      421 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
      481 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
      541 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
      601 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
      661 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
      721 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
      781 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
      841 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
      901 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
      961 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     1021 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     1081 CACCTGTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG
     1141 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC
     1201 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC
     1261 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT
     1321 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC
     1381 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT
     1441 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC
     1501 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG
     1561 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG
     1621 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG
     1681 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA
     1741 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT
     1801 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT CCCTGAATTC
     1861 GCATCTAGAT GGTAGAGCCA CAAACAGCCG GTACAAGCAA CGATCTCCAG GACCATCTGA
     1921 ATCATGCGCG GATGACACGA ACTCACGACG GCGATCACAG ACATTAACCC ACAGTACAGA
     1981 CACTGCGACA ACGTGGCAAT TCGTCGCAAT ACCGTCTCAC TGAACTGGCC GATAATTGCA
     2041 GACGAACGAG AAAGCATACT ATACTATTCG ACATTCCTTT CAATCCTGGA ATTAACAGTC
     2101 ACTTTTAAAA AAGACATCTA CCGTGAAGGT GCCGTAGAGT ATCGCGTTAC CATATCGCCA
     2161 AAAACTGATA TACGCCGCGG AAACCAGGCA AACAATTGAA AAGAAAAATT TTGAGGAACT
     2221 CTCTGCATCG AAGCCGTCTA GAGTTACCAC TAGTCAGATG CCGCGGGCAC TTGAGCACCT
     2281 CATGCACAGC AATAACACAA CACAATGGTT AGTAGCAACC TGAATTCGGT CATTGATGCA
     2341 TGCATGTGCC GTGAAGCGGG ACAACCAGAA AAGTCGTCTA TAAATGCCGG CACGTGCGAT
     2401 CATCGTGGCG GGGTTTTAAG AGTGCATATC ACAAATTGTC GCATTACCGC GGAACCGCCA
     2461 GATATTCATT ACTTGACGCA AAAGCGTTTG AAATAATGAC GAAAAAGAAG GAAGAAAAAA
     2521 AAAGAAAAAT ACCGCTTCTA GGCGGGTTAT CTACTGATCC GAGCTTCCAC TAGGATAGCA
     2581 CCCAAACACC TGCATATTTG GACGACCTTT ACTTACACCA CCAAAAACCA CTTTCGCCTC
     2641 TCCCGCCCCT GATAACGTCC ACTAATTGAG CGATTACCTG AGCGGTCCTC TTTTGTTTGC
     2701 AGCATGAGAC TTGCATACTG CAAATCGTAA GTAGCAACCT CTCAAGGTCA AAACTGTATG
     2761 GAAACCTTGT CACCTCACTT AATTCTAGCT AGCCTACCCT GCAAGTCAAG AGCTCTCCGT
     2821 GATTCCTAGC CACCTCAAGG TATGCCTCTC CCCGGAAACT GTGGCCTTTT CTGGCACACA
     2881 TGATCTCCAC GATTTCAACA TATAAATAGC TTTTGATAAT GGCAATATTA ATCAAATTTA
     2941 TTTTACTTCT TTCTTGTAAC ATCTCTCTTG TAATCCCTTA TTCCTTCTAG CTATTTTTCA
     3001 TAAAAAACCA AGCAACTGCT TATCAACACA CAAACACTAA ATCAAAAGAT CTATGTCCAA
     3061 TTTACTGACC GTACACCAAA ATTTGCCTGC ATTACCGGTC GATGCAACGA GTGATGAGGT
     3121 TCGCAAGAAC CTGATGGACA TGTTCAGGGA TCGCCAGGCG TTTTCTGAGC ATACCTGGAA
     3181 AATGCTTCTG TCCGTTTGCC GGTCGTGGGC GGCATGGTGC AAGTTGAATA ACCGGAAATG
     3241 GTTTCCCGCA GAACCTGAAG ATGTTCGCGA TTATCTTCTA TATCTTCAGG CGCGCGGTCT
     3301 GGCAGTAAAA ACTATCCAGC AACATTTGGG CCAGCTAAAC ATGCTTCATC GTCGGTCCGG
     3361 GCTGCCACGA CCAAGTGACA GCAATGCTGT TTCACTGGTT ATGCGGCGGA TCCGAAAAGA
     3421 AAACGTTGAT GCCGGTGAAC GTGCAAAACA GGCTCTAGCG TTCGAACGCA CTGATTTCGA
     3481 CCAGGTTCGT TCACTCATGG AAAATAGCGA TCGCTGCCAG GATATACGTA ATCTGGCATT
     3541 TCTGGGGATT GCTTATAACA CCCTGTTACG TATAGCCGAA ATTGCCAGGA TCAGGGTTAA
     3601 AGATATCTCA CGTACTGACG GTGGGAGAAT GTTAATCCAT ATTGGCAGAA CGAAAACGCT
     3661 GGTTAGCACC GCAGGTGTAG AGAAGGCACT TAGCCTGGGG GTAACTAAAC TGGTCGAGCG
     3721 ATGGATTTCC GTGTCTGGTG TAGCTGATGA TCCGAATAAC TACCTGTTTT GCCGGGTCAG
     3781 AAAAAATGGT GTTGCCGCGC CATCTGCCAC CAGCCAGCTA TCAACTCGCG CCCTGGAAGG
     3841 GATTTTTGAA GCAACTCATC GATTGATTTA CGGCGCTAAG GATGACTCTG GTCAGAGATA
     3901 CCTGGCCTGG TCTGGACACA GTGCCCGTGT CGGAGCCGCG CGAGATATGG CCCGCGCTGG
     3961 AGTTTCAATA CCGGAGATCA TGCAAGCTGG TGGCTGGACC AATGTAAATA TTGTCATGAA
     4021 CTATATCCGT AACCTGGATA GTGAAACAGG GGCAATGGTG CGCCTGCTGG AAGATGGCGG
     4081 ATCCTAATCA AGAGGATGTC AGAATGCCAT TTGCCTGAGA GATGCAGGCT TCATTTTTGA
     4141 TACTTTTTTA TTTGTAACCT ATATAGTATA GGATTTTTTT TGTCATTTTG TTTCTTCTCG
     4201 TACGAGCTTG CTCCTGATCA GCCTATCTCG CAGCTGATGA ATATCTTGTG GTAGGGGTTT
     4261 GGGAAAATCA TTCGAGTTTG ATGTTTTTCT TGGTATTTCC CACTCCTCTT CAGAGTACAG
     4321 AAGATTAAGT GGCTGCCAAT GAGACGACGG GGTCATCACG GCTCATCATG CGCCAAACAA
     4381 ATGTGTGCAA TACACGCTCG GATGACTGCA TGATGACCGC ACTGACTGGG GACAGCAGAT
     4441 CCACCTAAGC CTGTGAGAGA AGCAGACACC CGACAGATCA AGGCAGTTAA CTAGTGCACT
     4501 GCAGTACAAT CCTTCAGTAA TGTCTTGTTT CTTTTGTTGC AGTGGTGAGC CATTTTGACT
     4561 TCGTGAAAGT TTCTTTAGAA TAGTTGTTTC CAGAGGCCAA ACATTCCACC CGTAGTAAAG
     4621 TGCAAGCGTA GGAGAACCAA GACTGGCATA AATCAGGTAT AAGTGTCGAG CACTGGCAGG
     4681 TGATCTTCTG AAAGTTTCTA CTAGCAGATA AGATCCAGTA GTCATGCATA TGGCAACAAT
     4741 GTACCGTGTG GATCTAAGAA CGCGTCCTAC TAACCTTCGC ATTCGTTGGT CCAGTTTGTT
     4801 GTTATCGATC AACGTGACAA GGTTGTCGAT TCCGCGTAAG CATGCATACC CAAGGACGCC
     4861 TGTTGCAATT CCAAGTGAGC CAGTTCCAAC AATCTTTGTA ATATTAGAGC ACTTCATTGT
     4921 GTTGCGCTTG AAAGTAAAAT GCGAACAAAT TAAGAGATAA TCTCGAAACC GCGACTTCAA
     4981 ACGCCAATAT GATGTGCGGC ACACAATAAG CGTTCATATC CGCTGGGTGA CTTTCTCGCT
     5041 TTAAAAAATT ATCCGAAAAA ATTTTCTAGA GTGTTGTTAC TTTATACTTC CGGCTCGTAT
     5101 AATACGACAA GGTGTAAGGA GGACTAAACC ATGGCTAAAC TCACCTCTGC TGTTCCAGTC
     5161 CTGACTGCTC GTGATGTTGC TGGTGCTGTT GAGTTCTGGA CTGATAGACT CGGTTTCTCC
     5221 CGTGACTTCG TAGAGGACGA CTTTGCCGGT GTTGTACGTG ACGACGTTAC CCTGTTCATC
     5281 TCCGCAGTTC AGGACCAGGT TGTGCCAGAC AACACTCTGG CATGGGTATG GGTTCGTGGT
     5341 CTGGACGAAC TGTACGCTGA GTGGTCTGAG GTCGTGTCTA CCAACTTCCG TGATGCATCT
     5401 GGTCCAGCTA TGACCGAGAT CGGTGAACAG CCCTGGGGTC GTGAGTTTGC ACTGCGTGAT
     5461 CCAGCTGGTA ACTGCGTGCA TTTCGTCGCA GAAGAGCAGG ACTAAGCCGA GTAACTGACA
     5521 ATAAAAAGAT TCTTGTTTTC AAGAACTTGT CATTTGTATA GTTTTTTTAT ATTGTAGTTG
     5581 TTCTATTTTA ATCAAATGTT AGCGTGATTT ATATTTTTTT TCGCCTCGAC ATCATCTGCC
     5641 CAGATGCGAA GTTAAGTGCG CAGAAAGTAA TATCATGCGT CAATCGTATG TGAATGCTGG
     5701 TCGCTATACT GGAGTATGCA TTCGAGATAA GCTGGGGGAA CATTCGCGAA AATGAAACAA
     5761 GTCGGCTGTT ATAGTATATT TATTATAATA TTGAAAGATC TCAAAAGACT ACTTATTTTT
     5821 GAATGAACCA AGTATGAAAT CAACCTATTT GGGGTTGACC AAAATAAGTA AATATTAATT
     5881 GTCGAATGCA TCCGA
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