ID pET-28a-MBP-TEV-Cypc2; SV 1; circular; unknown; STD; UNC; 7740 BP. AC unknown; DT 29-SEP-2021 DE LMBP 12818. CC Accession number: LMBP 12818 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 29-SEP-2021 FH Key Location/Qualifiers FH FT promoter 209..250 FT /label="T7 promoter with lac operator" FT RBS 251..294 FT /label="5' UTR of T7 gene 10, incl. RBS" FT misc_feature 308..325 FT /label="His-tag" FT misc_feature 335..352 FT /label="thrombin recognition site" FT CDS 368..1477 FT /label="malE" FT misc_feature 1481..1501 FT /label="TEV recognition site" FT CDS 1505..2806 FT /label="Cyp c 2" FT misc_feature 2813..2830 FT /label="His-tag" FT terminator 2897..2944 FT /label="T7 terminator" FT rep_origin 2981..3436 FT /label="f1 ori" FT CDS complement(3528..4343) FT /label="Tn903 neomycin resistance" FT rep_origin 4835..5237 FT /label="pMB1 ori" FT CDS complement(5480..5671) FT /label="rop" FT CDS complement(6480..7562) FT /label="lacI" FT promoter complement(7563..7640) FT /label="lacI promoter, incl. RBS" SQ SEQUENCE 7740 AA; 2391948 MW; CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA 60 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT 120 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA 180 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG 240 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG 300 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCCATATGGC 360 TAGCGGTAAA ATCGAAGAAG GTAAACTGGT AATCTGGATT AACGGCGATA AAGGCTATAA 420 CGGTCTCGCT GAAGTCGGTA AGAAATTCGA GAAAGATACC GGAATTAAAG TCACCGTTGA 480 GCATCCGGAT AAACTGGAAG AGAAATTCCC ACAGGTTGCG GCAACTGGCG ATGGCCCTGA 540 CATTATCTTC TGGGCACACG ACCGCTTTGG TGGCTACGCT CAATCTGGCC TGTTGGCTGA 600 AATCACCCCG GACAAAGCGT TCCAGGACAA GCTGTATCCG TTTACCTGGG ATGCCGTACG 660 TTACAACGGC AAGCTGATTG CTTACCCGAT CGCTGTTGAA GCGTTATCGC TGATTTATAA 720 CAAAGATCTG CTGCCGAACC CGCCAAAAAC CTGGGAAGAG ATCCCGGCGC TGGATAAAGA 780 ACTGAAAGCG AAAGGTAAGA GCGCGCTGAT GTTCAACCTG CAAGAACCGT ACTTCACCTG 840 GCCGCTGATT GCTGCTGACG GGGGTTATGC GTTCAAGTAT GAAAACGGCA AGTACGACAT 900 TAAAGACGTG GGCGTGGATA ACGCTGGCGC GAAAGCGGGT CTGACCTTCC TGGTTGACCT 960 GATTAAAAAC AAACACATGA ATGCAGACAC CGATTACTCC ATCGCAGAAG CTGCCTTTAA 1020 TAAAGGCGAA ACAGCGATGA CCATCAACGG CCCGTGGGCA TGGTCCAACA TCGACACCAG 1080 CAAAGTGAAT TATGGTGTAA CGGTACTGCC GACCTTCAAG GGTCAACCAT CCAAACCGTT 1140 CGTTGGCGTG CTGAGCGCAG GTATTAACGC CGCCAGTCCG AACAAAGAGC TGGCGAAAGA 1200 GTTCCTCGAA AACTATCTGC TGACTGATGA AGGTCTGGAA GCGGTTAATA AAGACAAACC 1260 GCTGGGTGCC GTAGCGCTGA AGTCTTACGA GGAAGAGTTG GCGAAAGATC CACGTATTGC 1320 CGCCACCATG GAAAACGCCC AGAAAGGTGA AATCATGCCG AACATCCCGC AGATGTCCGC 1380 TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC AGACTGTCGA 1440 TGAAGCCCTG AAAGACGCGC AGACTCGTAT CACCAAGGGT GAAAATCTAT ACTTCCAAGG 1500 ATCTATGTCC ATCAGTAAGA TTCACGCTCG TGAGATCCTC GACTCCAGAG GAAACCCCAC 1560 TGTGGAGGTG GATCTGTACA CGGCTAAAGG ACGTTTCCGG GCAGCTGTTC CCAGCGGCGC 1620 TTCCACTGGA GTTCACGAGG CTCTGGAGCT CCGAGACGGA GACAAGACAC GCTACCTGGG 1680 CAAAGGTACC CAGAAGGCTG TGGACCATGT GAACAAAGAG ATCGCTCCCA AACTGATTGA 1740 GAAGAAGTTC AGCGTCGTGG AGCAGGAGAA GATCGACAAG TTCATGCTGG AACTCGATGG 1800 AACGGAGAAC AAATCTAAGT TTGGTGCTAA TGCGATCCTG GGTGTGAGTC TGGCCGTGTG 1860 TAAGGCAGGC GCGGCTGAGA AGGGCGTTCC TCTGTACCGC CACATCGCCG ACCTCGCCGG 1920 GAACAAAGAC GTGATCCTGC CGGTTCCTGC CTTCAACGTC ATCAACGGCG GCTCTCACGC 1980 CGGGAACAAG CTGGCCATGC AGGAGTTCAT GATCCTGCCG GTCGGAGCCA AGAACTTCCA 2040 CGAGGCCATG AGGATCGGCG CCGAGGTCTA CCACAACCTC AAGAACGTCA TCAAGGCCAA 2100 ATACGGCAAA GACGCCACTA ACGTGGGAGA CGAGGGCGGA TTCGCACCCA ACATCCTCGA 2160 GAACAACGAG GCTCTGGAGC TGCTGAAATC TGCCATCGAG AAGGCCGGCT ATCCTGACAA 2220 GATCATCATC GGGATGGACG TCGCCGCGTC CGAGTTCTTC AAGAATGGCA AATACGATCT 2280 GGACTTCAAG TCACCCGACG ACCCGAAGCG CCACATCACC GGAGATCAGC TGGGAGACTT 2340 ATACAAGAGC TTCATCAAGA ACTACCCCGT CCAGTCCATC GAGGATCCAT TTGACCAGGA 2400 TGACTGGGAG AACTGGAGCA AGTTCACGGG CTCAGTGGAC ATCCAGGTGG TGGGAGACGA 2460 TCTGACCGTC ACCAACCCCA AACGCATCCA GCAGGCATGT GAGAAGAAGG CCTGCAACTG 2520 CCTGCTGCTG AAGGTCAATC AGATCGGCTC CGTCACCGAG TCCATCCAGG CTTGTAAGCT 2580 GGCTCAGTCT AACGGTTGGG GTGTGATGGT CAGTCATCGC TCCGGTGAGA CGGAGGACAC 2640 CTTCATCGCT GACCTGGTGG TCGGTCTCTG CACAGGGCAG ATCAAGACCG GCGCCCCCTG 2700 CAGATCAGAG CGCTTGGCAA AGTACAACCA GCTGATGAGG ATCGAGGAGG AGCTCGGAGA 2760 CAAGGCCAAG TTCGCAGGCA AAGACTTCCG CCACCCCAAA CTCTGAGTCG AGCACCACCA 2820 CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC 2880 CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT 2940 TTTGCTGAAA GGAGGAACTA TATCCGGATT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA 3000 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 3060 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 3120 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 3180 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 3240 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 3300 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 3360 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA 3420 TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 3480 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAA TTAATTCTTA GAAAAACTCA 3540 TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC ATATTTTTGA 3600 AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG GATGGCAAGA 3660 TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT TAATTTCCCC 3720 TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA ATCCGGTGAG 3780 AATGGCAAAA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC ATTACGCTCG 3840 TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC CTGAGCGAGA 3900 CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG CAACCGGCGC 3960 AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC TTCTAATACC 4020 TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC AGGAGTACGG 4080 ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG TCTGACCATC 4140 TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA CTCTGGCGCA 4200 TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT GCCCGACATT ATCGCGAGCC 4260 CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT AGAGCAAGAC 4320 GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA AGCAGACAGT 4380 TTTATTGTTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC 4440 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 4500 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 4560 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT 4620 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 4680 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 4740 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 4800 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 4860 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 4920 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 4980 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 5040 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 5100 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC 5160 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG 5220 CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC 5280 ACACCGCAAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT 5340 ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG 5400 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG 5460 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC 5520 TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG 5580 CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA 5640 TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT 5700 TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG 5760 ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC 5820 GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG 5880 TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG 5940 CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG 6000 CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT 6060 CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA 6120 CGATCATGCG CACCCGTGGG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC 6180 GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG 6240 CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC 6300 AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG 6360 CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG 6420 GCATCGGTCG AGATCCCGGT GCCTAATGAG TGAGCTAACT TACATTAATT GCGTTGCGCT 6480 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 6540 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG 6600 ACGGGCAACA GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC 6660 ACGCTGGTTT GCCCCAGCAG GCGAAAATCC TGTTTGATGG TGGTTAACGG CGGGATATAA 6720 CATGAGCTGT CTTCGGTATC GTCGTATCCC ACTACCGAGA TATCCGCACC AACGCGCAGC 6780 CCGGACTCGG TAATGGCGCG CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC 6840 GCAGTGGGAA CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA 6900 CTCCAGTCGC CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG ATATTTATGC 6960 CAGCCAGCCA GACGCAGACG CGCCGAGACA GAACTTAATG GGCCCGCTAA CAGCGCGATT 7020 TGCTGGTGAC CCAATGCGAC CAGATGCTCC ACGCCCAGTC GCGTACCGTC TTCATGGGAG 7080 AAAATAATAC TGTTGATGGG TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA 7140 GTGCAGGCAG CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC 7200 CCACTGACGC GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC GACGCCGCTT 7260 CGTTCTACCA TCGACACCAC CACGCTGGCA CCCAGTTGAT CGGCGCGAGA TTTAATCGCC 7320 GCGACAATTT GCGACGGCGC GTGCAGGGCC AGACTGGAGG TGGCAACGCC AATCAGCAAC 7380 GACTGTTTGC CCGCCAGTTG TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC 7440 GCCGCTTCCA CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG 7500 GAAACGGTCT GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT TACTGGTTTC 7560 ACATTCACCA CCCTGAATTG ACTCTCTTCC GGGCGCTATC ATGCCATACC GCGAAAGGTT 7620 TTGCGCCATT CGATGGTGTC CGGGATCTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG 7680 GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG 7740 //