LOCUS SEGA001 2267 bp ds-DNA linear UNA 17-JUN-2021 DEFINITION LMBP 12837. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 2267) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Jun 17, 2021 from SnapGene 4.2.11 COMMENT SEGA landing pad: J23100-truncTetA-S2P11-rrnC FEATURES Location/Qualifiers source 1..2267 /organism="unspecified" /mol_type="genomic DNA" CDS 1..528 /codon_start=3 /label=glmS /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" misc_feature 538..594 /label=L3S2P11 Terminator misc_feature 604..638 /label=P-J23100 RBS 650..661 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 662..1774 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="VLPGLLRDIVHSDSIASHYGVLLALYALMQFLCAPVLGALSDRFG RRPVLLASLLGATIDYAIMATTPVLWILYAGRIVAGITGATGAVAGAYIADITDGEDRA RHFGLMSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGER RPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMS GLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIM ILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLIVTAIYAASASTW NGLAWIVGAALYLVCLPALRRGAWSRATST" misc_feature 1755..1804 /label=HR3 terminator 1785..1825 /label=rrnC misc_feature 1826..1875 /label=homology CDS 2020..2267 /codon_start=1 /label=pstS /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgcttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa 661 agtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg 721 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact 781 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga 841 ctacgcgatc atggcgacca cacccgtcct gtggattctc tacgccggac gcatcgtggc 901 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg 961 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc 1021 aggccccgtg gccgggggac tgttgggcgc catctccctg cacgcaccat tccttgcggc 1081 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa 1141 gggagagcgc cgtccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc 1201 gcggggcatg accattgtgg ccgcacttat gactgtcttc tttatcatgc aactcgtagg 1261 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac 1321 gatgagcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt 1381 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc 1441 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat 1501 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag 1561 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct 1621 aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg 1681 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg 1741 tcgcggtgca tggagccggg ccacctcgac ctgagacaat tgtccagata aaaaaaatcc 1801 ttagctttcg ctaaggatga tttctaaaat ttttctgtca tctcttcgtt attaatgttt 1861 gtaattgact gaatatcaac gcttatttaa atcagactga agactttatc tctctgtcat 1921 aaaactgtca tattccttac atataactgt cacctgtttg tcctattttg cttctcgtag 1981 ccaacaaaca atgctttatg aatcctccca ggagacatta tgaaagttat gcgtaccacc 2041 gtcgcaactg ttgtcgccgc gaccttatcg atgagtgctt tctctgtgtt tgcagaagca 2101 agcctgacag gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg ggctgacact 2161 taccagaaag aaaccggtaa taaagttaac taccagggta tcggttcttc cggtggcgta 2221 aaacagatta tcgctaatac cgttgatttt ggtgcctctg acgcgcc //