LOCUS SEGA018 3258 bp ds-DNA linear UNA 17-JUN-2021 DEFINITION LMBP 12854. ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 3258) AUTHORS Morten Norholm Group TITLE Direct Submission JOURNAL Exported Jun 17, 2021 from SnapGene 4.2.11 COMMENT SEGA landing pad: J23100-PelB-medium-sfGFP-T-tetA-S2P11-S3P22-rrnC FEATURES Location/Qualifiers source 1..3258 /organism="unspecified" /mol_type="genomic DNA" CDS 1..528 /codon_start=3 /label=glmS /translation="VHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPI ALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVR ARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVD QPRNLAKSVTVE" misc_feature 538..594 /label=L3S2P11 Terminator misc_feature 604..638 /label=P-J23100 RBS 650..661 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" misc_feature 662..676 /label=TIR CDS 668..733 /codon_start=1 /gene="pelB (fragment)" /product="leader peptide for secretion" /label=pelB signal sequence /translation="MKYLLPTAAAGLLLLAAQPAMA" CDS 734..1444 /codon_start=1 /label=sfGFP /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" misc_feature 1448..1496 /label=L3S3P22 Terminator (synthetic) promoter 1507..1535 /label=Promoter 3BCD2 CDS 1575..2765 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" misc_feature 2746..2795 /label=HR3 terminator 2776..2816 /label=rrnC misc_feature 2817..2866 /label=homology CDS 3011..3258 /codon_start=1 /label=pstS /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKWA DTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDA" ORIGIN 1 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggacaaacgc 61 attgaagcgc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc 121 gatcagtacc caatcgcgct ggaaggcgca ttgaagttga aagagatctc ttacattcac 181 gctgaagcct acgctgctgg cgaactgaaa cacggtccgc tggcgctaat tgatgccgat 241 atgccggtta ttgttgttgc accgaacaac gaattgctgg aaaaactgaa atccaacatt 301 gaagaagttc gcgcgcgtgg cggtcagttg tatgtcttcg ccgatcagga tgcgggtttt 361 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg 421 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc 481 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata aatggatctc 541 ggtaccaaat tccagaaaag agacgctttc gagcgtcttt tttcgttttg gtccgcgttg 601 cgcttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta aagaggagaa 661 atcaatgatg aagtacctgc tgccgaccgc tgctgctggt ctgctgctcc tcgctgccca 721 gccggcgatg gcccgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga 781 actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc 841 aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg 901 gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca 961 catgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac 1021 gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga 1081 taccctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct 1141 gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca 1201 aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca 1261 gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga 1321 caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca 1381 catggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta 1441 caaatgacca attattgaag gccgctaacg cggccttttt ttgtttctgg tctcccaaaa 1501 atttatttgc ttattaatca tccggctcgt ataatgtgtg gagggcccaa gttcacttaa 1561 aaaggagaat agttatgaaa tcgaacaatg cgctcatcgt catcctcggc accgtcaccc 1621 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg 1681 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc 1741 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc 1801 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc 1861 tgtggattct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 1921 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 1981 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 2041 ccatctccct gcacgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 2101 tgggctgctt cctaatgcag gagtcgcata agggagagcg ccgtccgatg cccttgagag 2161 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gaccattgtg gccgcactta 2221 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt 2281 tcggcgagga ccgctttcgc tggagcgcga cgatgagcgg cctgtcgctt gcggtattcg 2341 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg 2401 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt 2461 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg 2521 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc 2581 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca 2641 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg 2701 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 2761 cctgagacaa ttgtccagat aaaaaaaatc cttagctttc gctaaggatg atttctaaaa 2821 tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttattta 2881 aatcagactg aagactttat ctctctgtca taaaactgtc atattcctta catataactg 2941 tcacctgttt gtcctatttt gcttctcgta gccaacaaac aatgctttat gaatcctccc 3001 aggagacatt atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc 3061 gatgagtgct ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc 3121 tgcgccggtg tatgccaaat gggctgacac ttaccagaaa gaaaccggta ataaagttaa 3181 ctaccagggt atcggttctt ccggtggcgt aaaacagatt atcgctaata ccgttgattt 3241 tggtgcctct gacgcgcc //