LOCUS       pACYC177                3941 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 1346.
SOURCE      
  ORGANISM  
COMMENT     LMBP 1346
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 23-OCT-2007
            
COMMENT     VNTNAME|pACYC177|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
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COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2470 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 1 990 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 2 1593 0 3023 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindII" "gtyrac" 4 0 1 1 0 0 "")) 
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FEATURES             Location/Qualifiers
     rep_origin      901..1400
                     /vntifkey="33"
                     /label=p15A\ori
                     /note="E. coli p15A origin"
     CDS             1920..2735
                     /vntifkey="4"
                     /label=KAN
                     /note="Tn903 antibiotic resistance gene; coding sequence"
     CDS             3696..615
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1026 a       893 c       993 g      1029 t 
ORIGIN
        1 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
       61 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
      121 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
      181 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
      241 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca 
      301 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
      361 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
      421 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
      481 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
      541 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
      601 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 
      661 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
      721 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct 
      781 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 
      841 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 
      901 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 
      961 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 
     1021 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 
     1081 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 
     1141 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 
     1201 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     1261 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat 
     1321 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc 
     1381 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg 
     1441 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac 
     1501 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca 
     1561 acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa gaaggtgttg 
     1621 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt 
     1681 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg 
     1741 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat 
     1801 ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa 
     1861 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta 
     1921 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg 
     1981 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 
     2041 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 
     2101 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc 
     2161 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 
     2221 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 
     2281 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 
     2341 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 
     2401 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 
     2461 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 
     2521 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 
     2581 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 
     2641 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 
     2701 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac 
     2761 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt 
     2821 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa 
     2881 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc 
     2941 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc 
     3001 ttcacgaggc agacctcagc gctcaaagat gcaggggtaa aagctaaccg catctttacc 
     3061 gacaaggcat ccggcagttc aacagatcgg gaagggctgg atttgctgag gatgaaggtg 
     3121 gaggaaggtg atgtcattct ggtgaagaag ctcgaccgtc ttggccgcga cacgccgaca 
     3181 tgatccaact gataaaagag tttgatgctc agggtgtagc ggttcggttt attgacgacg 
     3241 ggatcagtac cgacggtgat atggggcaaa tggtggtcac catcctgtcg gctgtggcac 
     3301 aggctgaacg ccggaggatc ctagagcgca cgaatgaggg ccgacaggaa gcaaagctga 
     3361 aaggaatcaa atttggccgc aggcgtaccg tggacaggaa cgtcgtgctg acgcttcatc 
     3421 agaagggcac tggtgcaacg gaaattgctc atcagctcag tattgcccgc tccacggttt 
     3481 ataaaattct tgaagacgaa agggcctcgt gcatacgcct atttttatag gttaatgtca 
     3541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 
     3601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
     3661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
     3721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
     3781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
     3841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
     3901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt t 
//
