LOCUS       p416-YFP-N_for_N      7146 bp    DNA     circular UNA 3-MAY-2023
DEFINITION  LMBP 13777.
COMMENT     Accession number: LMBP 13777
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 3-MAY-2023
FEATURES             Location/Qualifiers
     CDS             417..1220
                     /label=URA3
     rep_origin      complement(1351..1806)
                     /label=f1\ori
     promoter        1977..1993
                     /label=T7\promoter
     terminator      complement(2014..2261)
                     /label=CYC1\terminator
     CDS             complement(2349..2864)
                     /label=yEVenus\fragment
     promoter        complement(2879..3283)
                     /label=ADH1\promoter
     promoter        complement(4374..4390)
                     /label=T3\promoter
     promoter        complement(4454..4537)
                     /label=lac\promoter
     rep_origin      complement(4810..5401)
                     /label=pMB1\ori
     CDS             complement(5572..6432)
                     /label=ampicillin\resistance
     rep_origin      6568..7092
                     /label=CEN6/ARSH4\ori
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATACCAC AGCTTTTCAA TTCAATTCAT CATTTTTTTT TTATTCTTTT TTTTGATTTC
      241 GGTTTCTTTG AAATTTTTTT GATTCGGTAA TCTCCGAACA GAAGGAAGAA CGAAGGAAGG
      301 AGCACAGACT TAGATTGGTA TATATACGCA TATGTAGTGT TGAAGAAACA TGAAATTGCC
      361 CAGTATTCTT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA TAAATCATGT
      421 CGAAAGCTAC ATATAAGGAA CGTGCTGCTA CTCATCCTAG TCCTGTTGCT GCCAAGCTAT
      481 TTAATATCAT GCACGAAAAG CAAACAAACT TGTGTGCTTC ATTGGATGTT CGTACCACCA
      541 AGGAATTACT GGAGTTAGTT GAAGCATTAG GTCCCAAAAT TTGTTTACTA AAAACACATG
      601 TGGATATCTT GACTGATTTT TCCATGGAGG GCACAGTTAA GCCGCTAAAG GCATTATCCG
      661 CCAAGTACAA TTTTTTACTC TTCGAAGACA GAAAATTTGC TGACATTGGT AATACAGTCA
      721 AATTGCAGTA CTCTGCGGGT GTATACAGAA TAGCAGAATG GGCAGACATT ACGAATGCAC
      781 ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA GAAGTAACAA
      841 AGGAACCTAG AGGCCTTTTG ATGTTAGCAG AATTGTCATG CAAGGGCTCC CTATCTACTG
      901 GAGAATATAC TAAGGGTACT GTTGACATTG CGAAGAGCGA CAAAGATTTT GTTATCGGCT
      961 TTATTGCTCA AAGAGACATG GGTGGAAGAG ATGAAGGTTA CGATTGGTTG ATTATGACAC
     1021 CCGGTGTGGG TTTAGATGAC AAGGGAGACG CATTGGGTCA ACAGTATAGA ACCGTGGATG
     1081 ATGTGGTCTC TACAGGATCT GACATTATTA TTGTTGGAAG AGGACTATTT GCAAAGGGAA
     1141 GGGATGCTAA GGTAGAGGGT GAACGTTACA GAAAAGCAGG CTGGGAAGCA TATTTGAGAA
     1201 GATGCGGCCA GCAAAACTAA AAAACTGTAT TATAAGTAAA TGCATGTATA CTAAACTCAC
     1261 AAATTAGAGC TTCAATTTAA TTATATCAGT TATTACCCTA TGCGGTGTGA AATACCGCAC
     1321 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT
     1381 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
     1441 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA
     1501 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG
     1561 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
     1621 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG
     1681 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA
     1741 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
     1801 GCGCGTCGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
     1861 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
     1921 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG CGCGCGTAAT
     1981 ACGACTCACT ATAGGGCGAA TTGGGTACCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA
     2041 AACCTTCTCA AGCAAGGTTT TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA
     2101 AAAAAGAAAA ATTTGAAATA TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA
     2161 AATAGGGACC TAGACTTCAG GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG
     2221 GGGAGGGCGT GAATGTAAGC GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG
     2281 ATAAGCTTGA TATCGAATTC CTGCAGCCCG GGGGATCCAG AACCTCCACC TCCGGAGCCA
     2341 CCGCCACCTT CAATGTTGTG TCTAATTTTG AAGTTAGCTT TGATACCATT CTTTTGTTTG
     2401 TCAGCAGTGA TGTAAACATT GTGAGAGTTA TAGTTGTATT CCAATTTGTG ACCTAAAATG
     2461 TTACCATCTT CTTTAAAATC AATACCTTTT AATTCGATTC TATTAACTAA GGTATCACCT
     2521 TCAAACTTGA CTTCAGCTCT GGTCTTGTAG TTACCGTCAT CTTTGAAAAA AATAGTTCTT
     2581 TCTTGAACAT AACCTTCTGG CATGGCAGAC TTGAAAAAGT CATGTTGTTT CATATGATCT
     2641 GGGTATCTAG CAAAACATTG CAAACCATAA CCTAAAGTAG TGACTAAGGT TGGCCATGGA
     2701 ACTGGCAATT TACCAGTAGT ACAAATCAAT TTTAAGGTCA ATTTACCGTA AGTAGCATCA
     2761 CCTTCACCTT CACCGGAGAC AGAAAATTTG TGACCATTAA CATCACCATC TAATTCAACC
     2821 AAAATTGGGA CAACACCAGT GAATAATTCT TCACCTTTAG ACATACTAGA GCGGCCAGCT
     2881 TGGAGTTGAT TGTATGCTTG GTATAGCTTG AAATATTGTG CAGAAAAAGA AACAAGGAAG
     2941 AAAGGGAACG AGAACAATGA CGAGGAAACA AAAGATTAAT AATTGCAGGT CTATTTATAC
     3001 TTGATAGCAA GACAGCAAAC TTTTTTTTAT TTCAAATTCA AGTAACTGGA AGGAAGGCCG
     3061 TATACCGTTG CTCATTAGAG AGTAGTGTGC GTGAATGAAG GAAGGAAAAA GTTTCGTGTG
     3121 CTTCGAGATA CCCCTCATCA GCTCTGGAAC AACGACATCT GTTGGTGCTG TCTTTGTCGT
     3181 TAATTTTTTC CTTTAGTGTC TTCCATCATT TTTTTTGTCA TTGCGGATAT GGTGAGACAA
     3241 CAACGGGGGA GAGAGAAAAG AAAAAAAAAG AAAAGAAGTT GCATGCGCCT ATTATTACTT
     3301 CAATAGATGG CAAATGGAAA AAGGGTAGTG AAACTTCGAT ATGATGATGG CTATCAAGTC
     3361 TAGGGCTACA GTATTAGTTC GTTATGTACC ACCATCAATG AGGCAGTGTA ATTGGTGTAG
     3421 TCTTGTTTAG CCCATTATGT CTTGTCTGGT ATCTGTTCTA TTGTATATCT CCCCTCCGCC
     3481 ACCTACATGT TAGGGAGACC AACGAAGGTA TTATAGGAAT CCCGATGTAT GGGTTTGGTT
     3541 GCCAGAAAAG AGGAAGTCCA TATTGTACAC CCGGAAACAA CAAAAGGATA TCCGAAATAT
     3601 TCCACGGTTT AGAAAAAAAT CGGAAAAGAG CGCGGAGGGG TGTTACCCCC CTTCTCTACT
     3661 AGCATTGGAC TTTAATTAAT ATATGTGCAT AGGAGAAGTG TAAAGTTCCC TTCCATATTG
     3721 TAACATAATA AAGTGCACAC CCAAATGAAT TGAAAGCGTA CTCAAACAGA CAACCATTTC
     3781 CAGTGTTGTA TGTACCTGTC TATTTATACT GGTAGCAACC CTATTGCTGT TTCCTCTTCA
     3841 AAGTACTCTA GCGGTTATGC GCGTCTCACC TTCAAGGTCA TGGTCGCTCT ATTGTTCGCA
     3901 CCACCGGCAA ACTCGCGTCT CGCAAGTCTT GGCTCATTCT TCTAGTATAC TCATGTTGCA
     3961 AATGCACTCA GGTTCTTTCG GCAACTTAAA TAATGACACC AGTTGTCGTG GTCGTCATCA
     4021 TCGCAACCCC AACCGGCATT CTTATTGCTT CTCCAATCTC GCCCCTTAGC GCAGGGTAAA
     4081 CCTTGGAAAA TGCAGGCGCA AAAAACTCCG CCGGGCACAG CCTCACGCCC AGCGTTATCG
     4141 CCGGGCCGGC AAGAGCGCGG GTCCGCCACA GAGTCAGCAT GATTGTGCAA TTGCGTAAAC
     4201 TCGTTTTTTC GGCGCCGCAA AGCCAAATAC ATCATATCAA CACTTTTCAC TTTATTTTTC
     4261 GTTCGACCCT TATATTTGTC TTTTGCCTTC ATGCTCCTTG ATTTCCTATT TCATTTACCA
     4321 TCATTTCTTC GATCCCGGNN NATCCGTACT TCAGAGCTCC AGCTTTTGTT CCCTTTAGTG
     4381 AGGGTTAATT GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
     4441 TCCGCTCACA ATTCCACACA ACATAGGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
     4501 CTAATGAGTG AGGTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
     4561 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG
     4621 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG
     4681 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
     4741 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
     4801 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     4861 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     4921 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     4981 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     5041 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     5101 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     5161 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     5221 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT
     5281 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     5341 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     5401 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     5461 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     5521 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     5581 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     5641 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     5701 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     5761 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     5821 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     5881 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     5941 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     6001 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     6061 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     6121 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     6181 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     6241 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     6301 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     6361 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     6421 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     6481 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     6541 ATTTCCCCGA AAAGTGCCAC CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT
     6601 TTAAATTTTT TAATATAAAT ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA
     6661 AAAAATAGTT TTTGTTTTCC GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT
     6721 ACCTTTTATC TTGCTCTTCC TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG
     6781 TGTAGAAGAC CACACACGAA AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG
     6841 TATATCTATT TAATCTGCTT TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT
     6901 TGAAATTTTT TAAACCTTTG TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT
     6961 TTATTTACTT TCTAAAATCC AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC
     7021 CCAAATTATT CCATCATTAA AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT
     7081 CCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
     7141 TTCGTC
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