LOCUS       pBR327                  3274 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 1453.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 1453
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 23-OCT-2007
            
            
            
COMMENT     VNTNAME|pBR327|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #1=(CRSite (CStringList) "HindII" "gtyrac" 4 0 2 654 0 2821 0 0 "")) 
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COMMENT        #3=(CFSignal (CObList) "pMB1 ori" 33 0 1 1429 1665 0 (CStringList) 
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COMMENT        #4=(CFSignal (CObList) "ampicillin" 4 0 1 2206 3066 0 (CStringList) 
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COMMENT                        "DNA Plasmid 'pBR327'" 1) 
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COMMENT                        "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                        "Creation Date: ? (no data)" 1) 
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COMMENT                        "Last Modified: 10/23/07 05:54" 1) 
COMMENT                   #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "length: 3274 bp" 1) 
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FEATURES             Location/Qualifiers
     CDS             86..1276
                     /vntifkey="4"
                     /label=tetracycline
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(1429..1665)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2206..3066)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      721 a       912 c       835 g       806 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg ccgcgttgct 
     1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 
     1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     2821 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     3181 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 
     3241 taggcgtatc acgaggccct ttcgtcttca agaa 
//
