LOCUS pBR327 3274 bp DNA circular 23-OCT-2007 DEFINITION LMBP 1453. SOURCE ORGANISM COMMENT Accession number: LMBP 1453 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium). Last modified: 23-OCT-2007 COMMENT VNTNAME|pBR327| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CWidget 0 (15 #5# 0) 1 2 0 0 #52# 29550 100) COMMENT (LOGPEN 458773 524290 37439744) 37678568 COMMENT (LOGFONT 61 23 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.91 0 "LMBP 1453" COMMENT "LMBP 1453" 65535 0.0769231 -2.48718 0 -10 7.4359 COMMENT 1.61538 Nil) COMMENT #127=(CLabel COMMENT (CWidget 0 (15 #6# 0) 1 2 0 0 #52# 21103008 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "Last modified: 23-OCT-2007" COMMENT "Last modified: 23-OCT-2007" 65535 -0.0769231 COMMENT -4.15385 0 -10 10.8462 1.05128 Nil)) (CObjectList)) COMMENT #128=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -449 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 2082602632 2082602632 (CObList))) FEATURES Location/Qualifiers CDS 86..1276 /vntifkey="4" /label=tetracycline /note="antibiotic resistance gene; coding sequence" rep_origin complement(1429..1665) /vntifkey="33" /label=pMB1\ori /note="origin plasmid" CDS complement(2206..3066) /vntifkey="4" /label=ampicillin /note="antibiotic resistance gene; coding sequence" BASE COUNT 721 a 912 c 835 g 806 t ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg ccgcgttgct 1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 2821 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 3181 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 3241 taggcgtatc acgaggccct ttcgtcttca agaa //