LOCUS       pATMIFNG                4822 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1597.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATMIFNG|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATMIFNG" 0 4822 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1633904500 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4822 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            1 1 1 1 "86..1276") 
COMMENT        #4=(CFSignal (CObList) "ORI" 33 0 1 1647 2049 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1647..2049)") 
COMMENT        #5=(CFSignal (CObList) "5UTR" 21 0 0 2907 2992 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2907..2992") 
COMMENT        #6=(CFSignal (CObList) "mIFNG" 4 0 0 2993 3460 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2993..3460") 
COMMENT        #7=(CFSignal (CObList) "3UTR" 21 0 0 3461 4070 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3461..4070")) (CObList) (CObList) (CObList) 
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COMMENT                         "DNA 'pATMIFNG'" 1) 
COMMENT                    #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 4822 bp" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                                  #26=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                  #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "mouse interferon \354 coding sequence" 1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   2993..3460" 1))) "mIFNG"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                  #39=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   2907..2992" 1))) "5UTR") 
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COMMENT                                       "3' UTR of the mouse interferon \354 gene" 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   3461..4070" 1))) "3UTR"))) 
COMMENT                         "Misc. Feature (2 total)") 
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FEATURES             Location/Qualifiers
     CDS             86..1276
                     /vntifkey="4"
                     /label=TET
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(1647..2049)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     misc_feature    2907..2992
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the mouse interferon ì gene"
     CDS             2993..3460
                     /vntifkey="4"
                     /label=mIFNG
                     /note="mouse interferon ì coding sequence"
     misc_feature    3461..4070
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the mouse interferon ì gene"
BASE COUNT     1141 a      1266 c      1204 g      1197 t        14 others
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 
     1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg 
     1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     2881 tttgcgcaac gttgttgcca ttgctgcagg gggnnccaag gnngatcctt tggaccctct 
     2941 gacttgagac agaagttctg ggcttctcct cctgcggcct agctctgaga caatgaacgc 
     3001 tacacactgc atcttggctt tgcagctctt cctcatggct gtttctggct gttactgcca 
     3061 cggcacagtc attgaaagcc tagaaagtct gaataactat tttaactcaa gtggcataga 
     3121 tgtggaagaa aagagtctct tcttggatat ctggaggaac tggcaaaagg atggtgacat 
     3181 gaaaatcctg cagagccaga ttatctcttt ctacctcaga ctctttgaag tcttgaaaga 
     3241 caatcaggcc atcagcaaca acataagcgt cattgaatca cacctgatta ctaccttctt 
     3301 cagcaacagc aaggcgaaaa aggatgcatt catgagtatt gccaagtttg aggtcaacaa 
     3361 cccacaggtc cagcgccaag cattcaatga gctcatccga gtggtccacc agctgttgcc 
     3421 ggaatccagc ctcaggaagc ggaaaaggag tcgctgctga ttcggggtgg ggaagagatt 
     3481 gtcccaataa gaataattct gccagcacta tttgaatttt taaatctaaa cctatttatt 
     3541 aatatttaaa actatttata tggagaatct attttagatg catcaaccaa agaagtattt 
     3601 atagtaacaa cttatatgtg ataagagtga attcctatta atatatgtgt tatttataat 
     3661 ttctgtctcc tcaactattt ctctttgacc aattaattat tctttctgac taattagcca 
     3721 agactgtgat tgcggggttg tatctggggg tgggggacag ccaagcggct gactgaactc 
     3781 agattgtagc ttgtaccttt acttcactga ccaataagaa acattcagag ctgcagtgac 
     3841 cccgggagtg ctgctgatgg gaggagatgt ctacactccg ggccagcgct ttaacagcag 
     3901 gccagacagc actcgaatgt gtcaggtagt aacaggctgt ccctgaaaga aagcagtgtc 
     3961 tcaagagact tgacactggt cttccctata cagctgaaaa ctgtgactac acccgaatga 
     4021 caaataactc gctcatttat agtttatcac tgtnnnnnnn nnnccccctg caggcatcgt 
     4081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     4141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     4201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     4261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     4321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 
     4381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     4441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     4501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     4561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     4621 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     4681 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     4741 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 
     4801 gaggcccttt cgtcttcaag aa 
//
