LOCUS       pATHIFNBg43             4390 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1656.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATHIFNBg43|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATHIFNBg43" 0 4390 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4390 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "hIFNB" 30 0 0 1 281 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1..281") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 282 354 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "282..354") 
COMMENT        #5=(CFSignal (CObList) "hIFNB" 4 0 0 355 918 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "355..918") 
COMMENT        #6=(CFSignal (CObList) "3UTR" 21 0 0 919 1107 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "919..1107") 
COMMENT        #7=(CFSignal (CObList) "ORI" 33 0 1 2380 2750 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2380..2750)") 
COMMENT        #8=(CFSignal (CObList) "AMP" 4 0 1 3322 4182 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3322..4182)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                         "DNA 'pATHIFNBg43'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 4390 bp" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                                       "Start: 355   End: 918 " 1) 
COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "human interferon \347 coding sequence" 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   355..918" 1))) "hIFNB") 
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COMMENT                                       "Start: 3322  End: 4182 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(3322..4182)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                  #38=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "5' UTR of the human interferon \347 gene" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #40=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   282..354" 1))) "5UTR") 
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COMMENT                                  #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of the human interferon\347 gene" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #45=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   919..1107" 1))) "3UTR"))) 
COMMENT                         "Misc. Feature (2 total)") 
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COMMENT                                       "Start: 1     End: 281 " 1) 
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COMMENT                                       "human interferon \347 promoter" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                         "Promoter Prokaryotic (1 total)") 
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COMMENT                                       1) 
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COMMENT                                       "origin plasmid" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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FEATURES             Location/Qualifiers
     promoter        1..281
                     /vntifkey="30"
                     /label=hIFNB
                     /note="human interferon ç promoter"
     misc_feature    282..354
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the human interferon ç gene"
     CDS             355..918
                     /vntifkey="4"
                     /label=hIFNB
                     /note="human interferon ç coding sequence"
     misc_feature    919..1107
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human interferonç gene"
     rep_origin      complement(2380..2750)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3322..4182)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1085 a      1115 c      1085 g      1105 t 
ORIGIN
        1 ttctcaggtc gtttgctttc ctttgctttc tcccaagtct tgttttacaa tttgctttag 
       61 tcattcactg aaactttaaa aaacattaga aaacctcaca gtttgtaaat ctttttccct 
      121 attatatata tcataagata ggagcttaaa taaagagttt tagaaactac taaaatgtaa 
      181 atgacatagg aaaactgaaa gggagaagtg aaagtgggaa attcctctga atagagagag 
      241 gaccatctca tataaatagg ccatacccat ggagaaagga cattctaact gcaacctttc 
      301 gaagcctttg ctctggcaca acaggtagta ggcgacactg ttcgtgttgt caacatgacc 
      361 aacaagtgtc tcctccaaat tgctctcctg ttgtgcttct ccactacagc tctttccatg 
      421 agctacaact tgcttggatt cctacaaaga agcagcaatt ttcagtgtca gaagctcctg 
      481 tggcaattga atgggaggct tgaatactgc ctcaaggaca ggatgaactt tgacatccct 
      541 gaggagatta agcagctgca gcagttccag aaggaggacg ccgcattgac catctatgag 
      601 atgctccaga acatctttgc tattttcaga caagattcat ctagcactgg ctggaatgag 
      661 actattgttg agaacctcct ggctaatgtc tatcatcaga taaaccatct gaagacagtc 
      721 ctggaagaaa aactggagaa agaagatttc accaggggaa aactcatgag cagtctgcac 
      781 ctgaaaagat attatgggag gattctgcat tacctgaagg ccaaggagta cagtcactgt 
      841 gcctggacca tagtcagagt ggaaatccta aggaactttt acttcattaa cagacttaca 
      901 ggttacctcc gaaactagcc tgtgcctctg ggactggaca attgcttcaa gcattcttca 
      961 accagcagat gctgtttaag tgactgatgg ctaatgtact gcatatgaaa ggacactaga 
     1021 agattttgaa atttttatta aattatgagt tatttttatt tatttaaatt ttattttgga 
     1081 aaataaatta tttttggtgc aaaagtcgat cctctacgcc ggacgcatcg tggccggcat 
     1141 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 
     1201 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 
     1261 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 
     1321 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 
     1381 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg 
     1441 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt 
     1501 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 
     1561 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg 
     1621 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc 
     1681 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat 
     1741 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt 
     1801 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc 
     1861 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 
     1921 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg 
     1981 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc 
     2041 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac 
     2101 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg 
     2161 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg 
     2221 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg 
     2281 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt 
     2341 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc tcttccgctt cctcgctcac 
     2401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     2461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     2521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     2581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     2641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     2701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg 
     2761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     2821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     2881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     2941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     3001 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     3061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     3121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     3181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     3241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     3301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     3361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     3421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     3481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     3541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     3601 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 
     3661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     3721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     3781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     3841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     3901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 
     3961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     4021 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     4081 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     4141 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     4201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     4261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 
     4321 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 
     4381 tcttcaagaa 
//
