LOCUS       pBRHIFNBg41             6331 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1659.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRHIFNBg41|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRHIFNBg41" 0 6331 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6331 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "1..281") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 282 354 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "282..354") 
COMMENT        #5=(CFSignal (CObList) "hIFNB" 4 0 0 355 918 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "355..918") 
COMMENT        #6=(CFSignal (CObList) "3UTR" 21 0 0 919 1118 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "919..1118") 
COMMENT        #7=(CFSignal (CObList) "3FR" 21 0 0 1119 1583 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1119..1583") 
COMMENT        #8=(CFSignal (CObList) "ORI" 33 0 1 3905 4305 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3905..4305)") 
COMMENT        #9=(CFSignal (CObList) "AMP" 4 0 1 4847 5707 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4847..5707)")) (CObList) (CObList) 
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COMMENT                          "DNA 'pBRHIFNBg41'" 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6331 bp" 1) 
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COMMENT                                        "Start: 355   End: 918 " 1) 
COMMENT                                   #28=(CLinePar 
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COMMENT                                        "human interferon \347 coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "3' UTR of the human interferon \347 gene" 
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FEATURES             Location/Qualifiers
     promoter        1..281
                     /vntifkey="30"
                     /label=hIFNB
                     /note="human interferon ç promoter"
     misc_feature    282..354
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the human interferon ç gene"
     CDS             355..918
                     /vntifkey="4"
                     /label=hIFNB
                     /note="human interferon ç coding sequence"
     misc_feature    919..1118
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human interferon ç gene"
     misc_feature    1119..1583
                     /vntifkey="21"
                     /label=3FR
                     /note="3' flanking region of the human interferon ç gene"
     rep_origin      complement(3905..4305)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4847..5707)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1595 a      1600 c      1534 g      1602 t 
ORIGIN
        1 ttctcaggtc gtttgctttc ctttgctttc tcccaagtct tgttttacaa tttgctttag 
       61 tcattcactg aaactttaaa aaacattaga aaacctcaca gtttgtaaat ctttttccct 
      121 attatatata tcataagata ggagcttaaa taaagagttt tagaaactac taaaatgtaa 
      181 atgacatagg aaaactgaaa gggagaagtg aaagtgggaa attcctctga atagagagag 
      241 gaccatctca tataaatagg ccatacccat ggagaaagga cattctaact gcaacctttc 
      301 gaagcctttg ctctggcaca acaggtagta ggcgacactg ttcgtgttgt caacatgacc 
      361 aacaagtgtc tcctccaaat tgctctcctg ttgtgcttct ccactacagc tctttccatg 
      421 agctacaact tgcttggatt cctacaaaga agcagcaatt ttcagtgtca gaagctcctg 
      481 tggcaattga atgggaggct tgaatactgc ctcaaggaca ggatgaactt tgacatccct 
      541 gaggagatta agcagctgca gcagttccag aaggaggacg ccgcattgac catctatgag 
      601 atgctccaga acatctttgc tattttcaga caagattcat ctagcactgg ctggaatgag 
      661 actattgttg agaacctcct ggctaatgtc tatcatcaga taaaccatct gaagacagtc 
      721 ctggaagaaa aactggagaa agaagatttc accaggggaa aactcatgag cagtctgcac 
      781 ctgaaaagat attatgggag gattctgcat tacctgaagg ccaaggagta cagtcactgt 
      841 gcctggacca tagtcagagt ggaaatccta aggaactttt acttcattaa cagacttaca 
      901 ggttacctcc gaaactgaag atctcctagc ctgtgcctct gggactggac aattgcttca 
      961 agcattcttc aaccagcaga tgctgtttaa gtgactgatg gctaatgtac tgcatatgaa 
     1021 aggacactag aagattttga aatttttatt aaattatgag ttatttttat ttatttaaat 
     1081 tttattttgg aaaataaatt atttttggtg caaaagtcaa catggcagtt ttaatttcga 
     1141 tttgatttat ataaccatcc atattataaa attgccaagt acctattagt tgttcttttt 
     1201 aaaatatacc tgcaaagtag tatactttct ggcccctgcc tttaaggaat ttaaaattca 
     1261 agaaagccat gatggaatat ataaggtaag agacaataag gggacctgaa ccttatgggg 
     1321 gaataaatat ggcatgaact gctgtgggat taaaagagaa aaggaaagct ggagggtctg 
     1381 gaactaaacc tggggttccc attcctccta ctgtgtgttc cagattctct catcataaag 
     1441 ttagaattga gctggccatc aggaatagcc agaggaatat gtcagctttt gtgttctccc 
     1501 taaccttccc cagttatttg ggggatcact ttgctcctcg aaagattttt aaataattat 
     1561 gtgcccccca ccatccctgc aagctttaat gcggtagttt atcacagtta aattgctaac 
     1621 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca 
     1681 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata 
     1741 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga 
     1801 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag 
     1861 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg 
     1921 tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg 
     1981 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc 
     2041 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg 
     2101 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac 
     2161 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga 
     2221 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 
     2281 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 
     2341 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 
     2401 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 
     2461 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 
     2521 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 
     2581 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 
     2641 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg 
     2701 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg 
     2761 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 
     2821 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc 
     2881 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat 
     2941 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc 
     3001 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 
     3061 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 
     3121 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 
     3181 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 
     3241 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 
     3301 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 
     3361 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 
     3421 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 
     3481 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 
     3541 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 
     3601 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     3661 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     3721 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 
     3781 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 
     3841 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 
     3901 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     3961 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     4021 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     4081 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     4141 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     4201 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     4261 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     4321 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     4381 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     4441 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     4501 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     4561 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     4621 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     4681 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     4741 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     4801 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     4861 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     4921 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     4981 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     5041 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     5101 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     5161 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     5221 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     5281 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     5341 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     5401 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     5461 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     5521 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     5581 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     5641 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     5701 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     5761 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     5821 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 
     5881 aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag atcacttcgc 
     5941 agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg 
     6001 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc 
     6061 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga 
     6121 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 
     6181 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 
     6241 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 
     6301 tcttgcccgc ctgatgaatg ctcatccgga a 
//
