LOCUS       pBRSTd844               3543 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1810.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRSTd844|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRSTd844" 0 3543 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1635017063 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3543 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "5UTR" 21 0 0 52 80 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "52..80") 
COMMENT        #4=(CFSignal (CObList) "COAT" 4 0 0 81 671 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "81..671") 
COMMENT        #5=(CFSignal (CObList) "3UTR" 21 0 0 672 1192 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "672..1192") 
COMMENT        #6=(CFSignal (CObList) "STNVd" 21 0 0 52 1192 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "52..1192") 
COMMENT        #7=(CFSignal (CObList) "ORI" 33 0 1 1525 1935 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1525..1935)") 
COMMENT        #8=(CFSignal (CObList) "AMP" 4 0 1 2475 3335 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2475..3335)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                         "DNA 'pBRSTd844'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3543 bp" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                  "Annotations") 
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COMMENT                                       "Start: 81    End: 671 " 1) 
COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "coat protein of STNV" 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   81..671" 1))) "COAT") 
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COMMENT                                       "Start: 2475  End: 3335 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(2475..3335)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       "Start: 52    End: 80  " 1) 
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COMMENT                                       "5' UTR of STNV RNA" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   52..80" 1))) "5UTR") 
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COMMENT                                  #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Satellite tobacco necrosis virus with deletion" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #45=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   52..1192" 1))) "STNVd") 
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COMMENT                                  #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of STNV RNA" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   672..1192" 1))) "3UTR"))) 
COMMENT                         "Misc. Feature (3 total)") 
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FEATURES             Location/Qualifiers
     misc_feature    52..80
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of STNV RNA"
     CDS             81..671
                     /vntifkey="4"
                     /label=COAT
                     /note="coat protein of STNV"
     misc_feature    672..1192
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of STNV RNA"
     misc_feature    52..1192
                     /vntifkey="21"
                     /label=STNVd
                     /note="Satellite tobacco necrosis virus with deletion"
     rep_origin      complement(1525..1935)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2475..3335)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      918 a       883 c       886 g       853 t         3 others
ORIGIN
        1 ttccggatcc ggccaagctt ggctctagag gctgcagggg gggggggggg gagtaaagac 
       61 aggaaacttt actgactaac atggcaaaac aacagaacaa caggcgaaaa tccgcaacaa 
      121 tgcgtgcagt gaagcgcatg ataaatacac acttggagca taaaaggttt gcactgatca 
      181 actcagggaa caccaatgca actgctggta cagtacaaaa tctgtccaac ggtataatcc 
      241 aaggagatga tatcaaccag agaagtggtg atcaagtgcg tatagtttca cataaacttc 
      301 acgtacgagg cactgccatc accgtcagcc agacctttag atttatctgg tttcgtgata 
      361 acatgaaccg tgggaccact cccacagttc ttgaggtgtt gaacactgcg aatttcatgt 
      421 cgcagtataa cccaatcacg ttgcagcaaa agagatttac aatactcaag gatgtaactc 
      481 tcaattgttc gctgacaggg gagagcatta aagatcggat aattaacctt ccaggacaac 
      541 tggtgaacta taatggagcg acggctgtag cagcctccaa tggtcccggc gcaatattta 
      601 tgttgcagat tggcgactcc ttggttggtc tgtgggactc ctcttatgag gctgtgtaca 
      661 cagatgcata atcccagagg ttcacaatgt tagtgatggg gcgctgaaag atgcgtagct 
      721 acccttctgg agccacttcc tggtggtaag cagaaatcca agggtacggt ggtacggtgg 
      781 aaagcagtcc cagctctgca ttgggaaccg gcttacaccc agcttagggc taaagtgtac 
      841 tacttgctca tttgtagtct aaatgagacg ttggcctcga cgtgtcgagg tggccnnntc 
      901 catggaatcc tccttgcaac aagaatatgt gcgccgtctg tgtttaaagc ggtatattaa 
      961 gtgcgccggc atatcgttgt ttggaccagg gccccacgcc ggttggtacc cgggtggctt 
     1021 cccctcgttc acagggcttt aggagatgat aaggtatagt tattagacaa atgcggacaa 
     1081 acctgaaaag ctcgctagtg gtgggctggc caagcgaaga acctcatcca ggtatagttc 
     1141 tacatgggaa atttggtacc atccaaactt ctatgaagtc ctcgactacc ccaaaccccc 
     1201 cccccccccc ccccccccct gcaggtcgac ctctagagcc aagcttggct gcctcgcgcg 
     1261 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 
     1321 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 
     1381 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 
     1441 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
     1501 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
     1561 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     1621 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     1681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     1741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     1801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     1861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
     1921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     1981 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     2041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     2101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
     2161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     2221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     2281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     2341 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     2401 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     2461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     2521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     2581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     2641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     2701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     2761 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 
     2821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     2881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     2941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     3001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     3061 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 
     3121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     3181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     3241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     3301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     3361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     3421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 
     3481 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 
     3541 gaa 
//
