LOCUS       pBRSTi163a              3655 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2031.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRSTi163a|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRSTi163a" 0 3655 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3655 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "5UTR" 21 0 0 52 80 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "52..80") 
COMMENT        #4=(CFSignal (CObList) "COATf" 4 0 0 81 214 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "81..214") 
COMMENT        #5=(CFSignal (CObList) "3UTR" 21 0 0 215 1304 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "215..1304") 
COMMENT        #6=(CFSignal (CObList) "STNVi" 21 0 0 52 1304 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "52..1304") 
COMMENT        #7=(CFSignal (CObList) "ORI" 33 0 1 1640 2050 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1640..2050)") 
COMMENT        #8=(CFSignal (CObList) "AMP" 4 0 1 2587 3447 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2587..3447)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                  "" 0 4) 
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COMMENT                         "DNA 'pBRSTi163a'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
COMMENT                    #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3655 bp" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                  "Annotations") 
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COMMENT                                       "Start: 81    End: 214 " 1) 
COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "part of STNV coat protein" 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   81..214" 1))) "COATf") 
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COMMENT                                       "Start: 2587  End: 3447 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(2587..3447)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       "Start: 52    End: 80  " 1) 
COMMENT                                  #38=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "5' UTR of STNV RNA" 1) 
COMMENT                                  #39=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #40=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   52..80" 1))) "5UTR") 
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COMMENT                                  #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Satellite tobacco necrosis virus with insertion" 
COMMENT                                       1) 
COMMENT                                  #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #45=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   52..1304" 1))) "STNVi") 
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COMMENT                                       "Start: 215   End: 1304" 1) 
COMMENT                                  #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of STNV RNA" 1) 
COMMENT                                  #49=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #50=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   215..1304" 1))) "3UTR"))) 
COMMENT                         "Misc. Feature (3 total)") 
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COMMENT                                       "Start: 1640  End: 2050 (Complementary)" 
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COMMENT                                       "origin plasmid" 1) 
COMMENT                                  #55=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #56=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                         "Replication Origin (1 total)"))) "Feature Map")))) 
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COMMENT          #58=(CWidgetStyle "Mark 1" 0 0 1 
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COMMENT               "@F bp (molecule @L bp)" 0) 
COMMENT          #63=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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FEATURES             Location/Qualifiers
     misc_feature    52..80
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of STNV RNA"
     CDS             81..214
                     /vntifkey="4"
                     /label=COATf
                     /note="part of STNV coat protein"
     misc_feature    215..1304
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of STNV RNA"
     misc_feature    52..1304
                     /vntifkey="21"
                     /label=STNVi
                     /note="Satellite tobacco necrosis virus with insertion"
     rep_origin      complement(1640..2050)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2587..3447)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      946 a       908 c       911 g       890 t 
ORIGIN
        1 ttccggatcc ggccaagctt ggctctagag gctgcagggg gggggggggg gagtaaagac 
       61 aggaaacttt actgactaac atggcaaaac aacagaacaa caggcgaaaa tccgcaacaa 
      121 tgcgtgcagt gaagcgcatg ataaatacac acttggagca taaaaggttt gcactgatca 
      181 actcagggaa caccaatgca actgctggta cagttccatg ggaattctac aaaatctgtc 
      241 caacggtata atccaaggag atgatatcaa ccagagaagt ggtgatcaag tgcgtatagt 
      301 ttcacataaa cttcacgtac gaggcactgc catcaccgtc agccagacct ttagatttat 
      361 ctggtttcgt gataacatga accgtgggac cactcccaca gttcttgagg tgttgaacac 
      421 tgcgaatttc atgtcgcagt ataacccaat cacgttgcag caaaagagat ttacaatact 
      481 caaggatgta actctcaatt gttcgctgac aggggagagc attaaagatc ggataattaa 
      541 ccttccagga caactggtga actataatgg agcgacggct gtagcagcct ccaatggtcc 
      601 cggcgcaata tttatgttgc agattggcga ctccttggtt ggtctgtggg actcctctta 
      661 tgaggctgtg tacacagatg cataatccca gaggttcaca atgttagtga tggggcgctg 
      721 aaagatgcgt agctaccctt ctggagccac ttcctggtgg taagcagaaa tccaagggta 
      781 cggtggtacg gtggaaagca gtcccagctc tgcattggga accggcttac acccagctta 
      841 gggctaaagt gtactacttg ctcatttgta gtctaaatga gacgttggcc tcgacgtgtc 
      901 gaggtggcct aagggattgg aacccctgat ggtcgtagtc gaatttcccg tgtttcattc 
      961 cgagtctctt ggtcataatg ccattagtag gtctagcact caacgtaact tcaaagatat 
     1021 cctccttgca acaagaatat gtgcgccgtc tgtgtttaaa gcggtatatt aagtgcgccg 
     1081 gcatatcgtt gtttggacca gggccccacg ccggttggta cccgggtggc ttcccctcgt 
     1141 tcacagggct ttaggagatg ataaggtata gttattagac aaatgcggac aaacctgaaa 
     1201 agctcgctag tggtgggctg gccaagcgaa gaacctcatc caggtatagt tctacatggg 
     1261 aaatttggta ccatccaaac ttctatgaag tcctcgacta ccccaaaccc cccccccccc 
     1321 cccccccccc ctgcaggtcg acctctagag ccaagcttgg ctgcctcgcg cgtttcggtg 
     1381 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
     1441 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 
     1501 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 
     1561 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 
     1621 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     1681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     1741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     1801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     1861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     1921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     1981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     2041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     2101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     2161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     2221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     2281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     2341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     2401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     2461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     2521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     2581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     2641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     2701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     2761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     2821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     2881 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     2941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     3001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     3061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     3121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     3181 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 
     3241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     3301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     3361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     3421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     3481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     3541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 
     3601 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaa 
//
