ID pSV23SMTNF standard; circular DNA; ; 5117 BP. DE LMBP 2171. CC Accession number: LMBP 2171 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 22-NOV-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 1..809 FT /vntifkey="4" FT /label=part\of\SV40\VP1 FT /note="part of the SV40 VP1 coding sequence" FT terminator complement(810..906) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator 810..906 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT misc_feature complement(907..1458) FT /vntifkey="21" FT /label=3'\UTR\of\SV40\small\t-antigen FT /note="3' UTR of the SV40 small t-antigen" FT intron complement(1459..1525) FT /vntifkey="15" FT /label=intron\of\SV40\small\t-antigen FT /note="intron of the SV40 small t-antigen" FT CDS complement(1526..1600) FT /vntifkey="4" FT /label=part\of\SV40\small\t-antigen FT /note="part of the SV40 small t-antigen" FT misc_feature complement(1624..1781) FT /vntifkey="21" FT /label=3'\UTR\of\mTNF FT /note="3' UTR of the mouse tumor necrosis factor gene" FT CDS complement(1782..2252) FT /vntifkey="4" FT /label=mTNF\mature\sequence FT /note="mouse tumor necrosis factor mature sequence" FT CDS complement(2253..2489) FT /vntifkey="4" FT /label=mTNF\presequence FT /note="mouse tumor necrosis factor presequence" FT misc_feature complement(2490..2517) FT /vntifkey="21" FT /label=5'\UTR\of\mTNF FT /note="5' UTR of the mouse tumor necrosis factor gene" FT promoter complement(2603..2884) FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin complement(2571..2646) FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT rep_origin complement(3106..3508) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4049..4909) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 5117 BP; 1338 A; 1159 C; 1283 G; 1337 t; ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg 60 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac 120 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg 180 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc 240 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc 300 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa 360 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg 420 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct 480 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca 540 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc 600 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga 660 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg 720 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt 780 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg 840 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca 900 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc 960 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc 1020 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt 1080 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact 1140 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc 1200 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc 1260 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt 1320 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat 1380 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc 1440 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta 1500 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa 1560 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgaccgg gggggggggg 1620 ggggttgaaa gctctgagca cagagttgga ctctgagcca taatcccctt tctaagttag 1680 aaggacacag actgggggct ctgaggagta gacaataaag gggtcagagt aaaggggtca 1740 gagtgggggc tgggtagaga atggatgaac acccattccc ttcacagagc aatgactcca 1800 aagtagacct gcccggactc cgcaaagtct aagtacttgg gcagattgac ctcagcgctg 1860 agttggtccc ccttctccag ctggaagact cctcccaggt atatgggctc ataccagggt 1920 ttgagctcag ccccctcagg ggtgtccttg gggcaggggc tcttgacggc agagaggagg 1980 ttgactttct cctggtatga gatagcaaat cggctgacgg tgtgggtgag gagcacgtag 2040 tcggggcagc cttgtccctt gaagagaacc tgggagtaga caaggtacaa cccatcggct 2100 ggcaccacta gttggttgtc tttgagatcc atgccgttgg ccaggagggc gttggcgcgc 2160 tggctcagcc actccagctg ctcctccact tggtggtttg ctacgacgtg ggctacaggc 2220 ttgtcactcg aattttgaga agatgatctg agtgtgaggg tctgggccat agaactgatg 2280 agagggaggc catttgggaa cttctcatcc ctttggggac cgatcacccc gaagttcagt 2340 agacagaaga gcgtggtggc ccctgccaca agcaggaatg agaagaggct gagacatagg 2400 caccgcctgg agttctggaa gccccccatc ttttggggga gtgcctcttc tgccagttcc 2460 acgtcgcgga tcatgctttc tgtgctcatg gtgtcttttc tggagggaga tgtggcgggt 2520 cgactctaga gccaagcttg agctttttgc aaaagcctag gcctccaaaa aagcctcctc 2580 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa 2640 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg 2700 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc 2760 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt 2820 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc 2880 acagtcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag 2940 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga 3000 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt 3060 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgctct tccgcttcct 3120 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3180 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3240 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3300 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3360 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3420 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3480 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3540 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3600 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3660 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3720 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3780 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3840 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3900 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3960 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4020 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4080 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4140 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4200 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4260 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4320 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 4380 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 4440 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 4500 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 4560 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 4620 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 4680 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 4740 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 4800 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 4860 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4920 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 4980 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5040 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 5100 cctttcgtct tcaagaa 5117 //