LOCUS       pATMx                   4112 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2390.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATMx|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATMx" 0 4112 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4112 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "Mx" 30 0 1 31 476 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "complement(31..476)") 
COMMENT        #4=(CFSignal (CObList) "TET" 4 0 0 540 1730 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "540..1730") 
COMMENT        #5=(CFSignal (CObList) "ORI" 33 0 1 2100 2500 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2100..2500)") 
COMMENT        #6=(CFSignal (CObList) "AMP" 4 0 1 3044 3904 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3044..3904)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT            (CObjectList 
COMMENT             #8=(CRefLinePar 
COMMENT                 (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pATMx" 2) 5 "" 0 4) 
COMMENT             #9=(CFolderPar 
COMMENT                 (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                  (CObjectList 
COMMENT                   #10=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DNA 'pATMx'" 
COMMENT                        1) 
COMMENT                   #11=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                        1) 
COMMENT                   #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Creation Date: ? (no data)" 1) 
COMMENT                   #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Last Modification Date: ? (no data)" 1) 
COMMENT                   #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "length: 4112 bp" 1) 
COMMENT                   #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "storage type: Basic" 1) 
COMMENT                   #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "form: Circular" 1))) "General Description") 
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COMMENT                   (CObjectList 
COMMENT                    #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
COMMENT             #19=(CRefLinePar 
COMMENT                  (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                  0 0) 
COMMENT             #20=(CFolderPar 
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COMMENT                  "Annotations") 
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COMMENT                   (CObjectList 
COMMENT                    #22=(CFolderPar 
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COMMENT                          (CObjectList 
COMMENT                           #23=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 540 (3 #4# 0) 1 2 2 0 327) 
COMMENT                                 (CObjectList 
COMMENT                                  #24=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 540   End: 1730" 1) 
COMMENT                                  #25=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #26=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   540..1730" 1))) "TET") 
COMMENT                           #28=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 3044 (3 #6# 0) 1 2 2 0 327) 
COMMENT                                 (CObjectList 
COMMENT                                  #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 3044  End: 3904 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #30=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(3044..3904)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
COMMENT                    #33=(CFolderPar 
COMMENT                         (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                          (CObjectList 
COMMENT                           #34=(CFolderPar 
COMMENT                                (CGroupPar (CParagraph 31 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                 (CObjectList 
COMMENT                                  #35=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 31    End: 476  (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #36=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "mouse Mx promoter" 1) 
COMMENT                                  #37=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #38=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(31..476)" 1))) "Mx"))) 
COMMENT                         "Promoter Prokaryotic (1 total)") 
COMMENT                    #39=(CFolderPar 
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COMMENT                          (CObjectList 
COMMENT                           #40=(CFolderPar 
COMMENT                                (CGroupPar 
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COMMENT                                 (CObjectList 
COMMENT                                  #41=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 2100  End: 2500 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #42=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "origin plasmid" 1) 
COMMENT                                  #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(2100..2500)" 1))) "ORI"))) 
COMMENT                         "Replication Origin (1 total)"))) "Feature Map")))) 
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COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT               "@F bp (molecule @L bp)" 0) 
COMMENT          #51=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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COMMENT          #78=(CWidgetStyle "~Scrap" 0 0 1 
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FEATURES             Location/Qualifiers
     promoter        complement(31..476)
                     /vntifkey="30"
                     /label=Mx
                     /note="mouse Mx promoter"
     CDS             540..1730
                     /vntifkey="4"
                     /label=TET
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(2100..2500)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3044..3904)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      919 a      1151 c      1062 g       980 t 
ORIGIN
        1 ttcgagctcg cccggggatc ctctagagtc ctgccagggc tcagacctct ttaaatagtg 
       61 ctggctccgc cccttccaga ggagaattga aaccgtgaaa aagcctgagc ttgctgtgcc 
      121 cacaccccca ccacatatga aaggagctca gaaacgaaac tcaccctgag gggccttggc 
      181 ttggtactga ggttgggaaa tcgatattgt aggctcagcc caccaagaca catgcagtat 
      241 ggacaccagt tcagatggct gcctgctttc ccaggaccct tccagctctg tgctgagcct 
      301 tcagcctgac ctcctctcag ttgaacacaa cctagactaa ctttctcttt tcactggatt 
      361 cttccagctg cactaggaag ggtcagagac aaaccatacc agaatcactg aggagccctg 
      421 ccagcccttc tgggtccctt gtgactacag gtttagaggt ctgagcactg ctactgcagc 
      481 ccaagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta 
      541 tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca 
      601 taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca 
      661 tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac 
      721 ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac 
      781 ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg 
      841 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 
      901 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 
      961 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 
     1021 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 
     1081 tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca 
     1141 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 
     1201 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 
     1261 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 
     1321 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 
     1381 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct 
     1441 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc 
     1501 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg 
     1561 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg 
     1621 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct 
     1681 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg 
     1741 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag 
     1801 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag 
     1861 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt 
     1921 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga 
     1981 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc 
     2041 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc 
     2101 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     2161 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     2221 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     2281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     2341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     2401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     2461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     2521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     2581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     2641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     2701 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
     2761 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     2821 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     2881 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     2941 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     3001 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     3061 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     3121 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     3181 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     3241 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     3301 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg 
     3361 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     3421 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     3481 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     3541 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     3601 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     3661 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     3721 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     3781 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     3841 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     3901 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     3961 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     4021 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 
     4081 ggcgtatcac gaggcccttt cgtcttcaag aa 
//
