LOCUS       pCDM8      4356 bp    DNA     circular UNA 11-Mar-2022
DEFINITION  LMBP 2393.
COMMENT     Accession number: LMBP 2393
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2022
            NGS data implemented
FEATURES             Location/Qualifiers
     rep_origin      complement(36..111)
                     /label=SV40\ori
     rep_origin      complement(262..375)
                     /label=polyoma\ori
     terminator      complement(1067..1163)
                     /label=SV40\polyA\early
     terminator      1067..1163
                     /label=SV40\polyA\late
     misc_feature    complement(1164..1562)
                     /label=3'\UTR\of\SV40\small\t-antigen
     intron          complement(1563..1629)
                     /label=intron\of\SV40\small\t-antigen
     CDS             complement(1630..1704)
                     /label=SV40\small\t-antigen\fragment
     promoter        complement(2177..2193)
                     /label=T7\promoter
     promoter        complement(2238..2821)
                     /label=hCMV-IE\promoter\and\enhancer
     misc_feature    2976..3175
                     /label=supF
     rep_origin      3296..3754
                     /label=M13\ori
     rep_origin      complement(3773..4005)
                     /label=pMB1\ori
ORIGIN
        1 GAATTAGCTT TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA
       61 GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG
      121 GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTA AGACCCGCAC
      181 CCGTTCCCAA ACTGCTTATT AAAGGGGGTA TGGAGGTGCT GGACCTTGTG ACAGGGCCAG
      241 ACAGTGTGAC AGAAATAGAA GCAGTCCTGT TTTGACAAGT TGCCTCTGGA AGCCTCTACA
      301 ATGCCTCTCT TCTTTTTCTC CAGAGTAAGC GGAGGCCAGG GGCCCCCGGC CTCTGCTTAA
      361 TACTAAAAAA AACAGCTGTT GTCATAGTAA TGATTGGGTG GAAACATTCT AGGCCTGGGT
      421 GGAGAGGCTT TTTGCTTCCT CTTGCAAAAC CACACTGCCC TCTGGAGGGC AGTTGCCTAG
      481 CAACTAATTA AAAGAGGATG TCGCACGGCC AGCTGCGGTC AGTTAGTCAC TTCCTGCTTA
      541 ACTGACTTGA CATTTTCTAT TTTAAGAGTC GGGAGGAAAA TTACTGTGTT GGAGGCCCTC
      601 CGCCATCTTC TGAAGCTGAT CAAGTAAGTG AATTTTCAAA ATGGGAGCCG CACTGACTAT
      661 TCTAGTAGAC CTCATCGAGG GATTAGCTGA AGTGTCTACC CTTACGGGAC TCTCGGCAGA
      721 AGCTATTTTA TCTGGAGAAG CCCTCGCTGC CCTTGATGGC GAAATTACAG CTCTGACTTT
      781 GGAGGGTGTA ATGAGTTCGG AGACAGCCCT AGCAACTATG GGTATTTCAG AGGAGGTGTA
      841 TGGGTTTGTC AGTACTGTGC CTGTGTTTGT AAATCGAACA GCGGGGGCTA TATGGCTGAT
      901 GCAGACAGTT CAAGGTGCCT CTACTATTTC CCTAGGAATA CAGCGGTACC TACACAACGA
      961 AGAGGTCCCT ACTGTAAATA GAAATATGGC GTTGATACCA TGGCGGGATC CAGACATGAT
     1021 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
     1081 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
     1141 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
     1201 TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTCT AGTCAAGGCA
     1261 CTATACATCA AATATTTTTC CATAATTTTC TTGTATAGCA GTGCAGCTTT TTCCTTTGTG
     1321 GTGTAAATAG CAAAGCAAGC AAGAGTTCTA TTACTAAACA CAGCATGACT CAAAAAACTT
     1381 AGCAATTCTG AAGGAAAGTC CTTGGGGTCT TCTACCTTTC TCTTCTTTTT TGGAGGAGTA
     1441 GAATGTTGAG AGTCAGCAGT AGCCTCATCA TCACTAGATG GCATTTCTTC TGAGCAAAAC
     1501 AGGTTTTCCT CATTAAAGGC ATTCCACCAC TGCTCCCATT CATCAGTTCC ATAGGTTGGA
     1561 ATCTAAAATA CACAAACAAT TAGAATCAGT AGTTTAACAC ATTATACACT TAAAAATTTT
     1621 ATATTTACCT TAGAGCTTTA AATCTCTGTA GGTAGTTTGT CCAATTATGT CACACCACAG
     1681 AAGTAAGGTT CCTTCACAAA GATCCTCTAG AGTCGCGGCC GCGACCTGCA GGCGCAGAAC
     1741 TGGTAGGTAT GGAAGATCCC TCGAGATCCA TTGTGCTGGC GGTAGGCGAG CAGCGCCTGC
     1801 CTGAAGCTGC GGGCATTCCC GATCAGAAAT GAGCGCCAGT CGTCGTCGGC TCTCGGCACC
     1861 GAATGCGTAT GATTCTCCGC CAGCATGGCT TCGGCCAGTG CGTCGAGCAG CGCCCGCTTG
     1921 TTCCTGAAGT GCCAGTAAAG CGCCGGCTGC TGAACCCCCA ACCGTTCCGC CAGTTTGCGT
     1981 GTCGTCAGAC CGTCTACGCC GACCTCGTTC AACAGGTCCA GGGCGGCACG GATCACTGTA
     2041 TTCGGCTGCA ACTTTGTCAT GCTTGACACT TTATCACTGA TAAACATAAT ATGTCCACCA
     2101 ACTTATCAGT GATAAAGAAT CCGCGCCAGC ACAATGGATC TCGAGGTCGA GGGATCTCTA
     2161 GAAGCTTGGG TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCAGTA AGCAGTGGGT
     2221 TCTCTAGTTA GCCAGAGAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC
     2281 ATTTGCGTCA ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC
     2341 CAAAACAAAC TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC
     2401 CGCTATCCAC GCCCATTGAT GTACTGCCAA AACCGCATCA CCATGGTAAT AGCGATGACT
     2461 AATACGTAGA TGTACTGCCA AGTAGGAAAG TCCCATAAGG TCATGTACTG GGCATAATGC
     2521 CAGGCGGGCC ATTTACCGTC ATTGACGTCA ATAGGGGGCG TACTTGGCAT ATGATACACT
     2581 TGATGTACTG CCAAGTGGGC AGTTTACCGT AAATACTCCA CCCATTGACG TCAATGGAAA
     2641 GTCCCTATTG GCGTTACTAT GGGAACATAC GTCATTATTG ACGTCAATGG GCGGGGGTCG
     2701 TTGGGCGGTC AGCCAGGCGG GCCATTTACC GTAAGTTATG TAACGCGGAA CTCCATATAT
     2761 GGGCTATGAA CTAATGACCC CGTAATTGAT TACTATTAAT AACTAGTCAA TAATCAATGT
     2821 CAACGCGTAT ATCTGGCCCG TACATCGCGA AGCAGCGCAA AACGCCTAAC CCTAAGCAGA
     2881 TTCTTCATGC AATTGTCGGT CAAGCCTTGC CTTGTTGTAG CTTAAATTTT GCTCGCGCAC
     2941 TACTCAGCGA CCTCCAACAC ACAAGCAGGG AGCAGCTTTC TCAACGTAAC ACTTTACAGC
     3001 GGCGCGTCAT TTGATATGAT GCGCCCCGCT TCCCGATAAG GGAGCAGGCC AGTAAAAGCA
     3061 TTACCTGTGG TGGGGTTCCC GAGCGGCCAA AGGGAGCAGA CTCTAAATCT GCCGTCATCG
     3121 ACTTCGAAGG TTCGAATCCT TCCCCCACCA CCATCACTTT CAAAAGTCCG AAAGACCGCG
     3181 GTCCAGCTGG CGTACCGTTC CTGTCTAAAA TCCCTTTAAT CGGCCTCCTG TTTAGCTCCC
     3241 GCTCTGATTC TAACGAGGAA AGCACGTTAT ACGTGCTCGT CAAAGCAACC ATAGTACGCG
     3301 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
     3361 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
     3421 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
     3481 TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCGGCCA
     3541 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
     3601 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG GCTATTCTTT TGATTTATAA
     3661 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     3721 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTCTAGCT AGAAGCTAGC TTGCGTTGCT
     3781 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     3841 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     3901 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     3961 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     4021 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     4081 CGGTAACTAT CTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     4141 ACTGGTAACT GGCTTCAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     4201 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     4261 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     4321 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCC
//
