LOCUS       pEMBLyex4               8800 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 2423.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2423
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
            
COMMENT     VNTDATE|446400346|
COMMENT     VNTNAME|pEMBLyex4|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEMBLyex4" 0 8800 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 1668612217 0 "" 0 "" "" "" "" "" "" "pEMBLyex4-a" 
COMMENT             "" 0 0 1 150 1 150 1 150 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 150 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 1 1 0 34078879 0 "" 0 "GAC" "" "" "" "" "" "" "" 0 0 1 3 
COMMENT             151 153 1 3 0 "gac" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 1920090468 0 "" 0 "" "" "" "" "" "" "pEMBLyex4-a" 
COMMENT             "" 0 0 1 3 154 156 151 153 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 151 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 153 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #3=(CFragment 1 1 0 21823596 0 "" 0 "GTC" "" "" "" "" "" "" "" 0 0 1 3 
COMMENT             157 159 1 3 0 "gtc" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #4=(CFragment 3 1 0 1096810496 0 "" 0 "" "" "" "" "" "" "pEMBLyex4-a" 
COMMENT             "" 0 0 1 8641 160 8800 154 8794 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 154 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AspI" "Tth111I" "EcoRI" "HindIII" "XmnI" "XbaI" "FspI" 
COMMENT         "AflIII" "BstXI" "AflII" "BstEII" "ApaI" "StuI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 8800 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #5=(CRSite (CStringList) "AspI" "gacnnngtc" 5 0 1 155 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 1 155 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2431 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 4779 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 2435 0 4042 0 8408 0 
COMMENT            0 "") #10=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4803 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "FspI" "tgcgca" 4 0 3 3978 0 4491 0 8031 0 0 
COMMENT             "") 
COMMENT        #12=(CRSite (CStringList) "AflIII" "acrygt" 2 0 3 2331 0 6155 0 6917 0 0 
COMMENT             "") 
COMMENT        #13=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 2361 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 2753 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 3033 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 5964 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "StuI" "aggcct" 4 0 1 5902 0 0 "")) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "ampicillin" 4 0 1 7735 8595 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7735..8595)") 
COMMENT        #19=(CFSignal (CObList) "pMB1 ori" 33 0 1 6793 7195 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6793..7195)") 
COMMENT        #20=(CFSignal (CObList) "URA3" 4 0 1 5535 6338 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5535..6338)") 
COMMENT        #21=(CFSignal (CObList) "GAL10/CYC1 promoter" 29 0 1 4909 5316 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4909..5316)") 
COMMENT        #22=(CFSignal (CObList) "2mu inverted repeat" 21 0 0 4176 4778 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4176..4778") 
COMMENT        #23=(CFSignal (CObList) "FLP terminator" 43 0 1 4491 4595 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4491..4595)") 
COMMENT        #24=(CFSignal (CObList) "2mu ori" 33 0 1 3429 4409 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3429..4409)") 
COMMENT        #25=(CFSignal (CObList) "defective LEU2" 4 0 1 1977 3071 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1977..3071)") 
COMMENT        #26=(CFSignal (CObList) "f1 ori" 33 0 1 754 1209 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(754..1209)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #27=(CFSignal (CObList) "LMBP 2423" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #28=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pEMBLyex4'" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 2423." 
COMMENT                          1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 05/14/07 04:05" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/29/07 11:08" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8800 bp" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 14-MAY-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                          "#2: SEQUENCE GAC") 
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COMMENT                                 "molecule position: from 157 to 159" 1))) 
COMMENT                          "#4: SEQUENCE GTC") 
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COMMENT                                 "parent position: from 154 to 8794" 1) 
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COMMENT                                 "original length: 8641" 1) 
COMMENT                            #73=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
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COMMENT                          "#5: FRAGMENT of <a href=\"vnti:dnarna/pEMBLyex4-a?sel=154-8794\">pEMBLyex4-a</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #78=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1977 (3 #25# 0) 1 2 2 0 327) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1977  End: 3071 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "defective LEU2 coding sequence" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1977..3071)" 1))) 
COMMENT                                 "defective LEU2") 
COMMENT                            #85=(CFolderPar 
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COMMENT                                  (CParagraph 5535 (3 #20# 0) 1 2 2 0 327) 
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COMMENT                                        "Start: 5535  End: 6338 (Complementary)" 
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COMMENT                                   #87=(CLinePar 
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COMMENT                                        "S. cerevisiae uracil 3 coding sequence" 
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COMMENT                                        "   complement(5535..6338)" 1))) "URA3") 
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COMMENT                                        "Start: 7735  End: 8595 (Complementary)" 
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COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                   #93=(CLinePar 
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COMMENT                                        "   complement(7735..8595)" 1))) 
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COMMENT                                        "Start: 4176  End: 4778" 1) 
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COMMENT                                 "2mu inverted repeat"))) 
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COMMENT                                     #104=(CLinePar 
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COMMENT                                           "UAS-G: 5316-5169, GAL10: 5168-5091,CYC1: 5086-4909, (all 3 compl.)" 
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COMMENT                                           "   complement(4909..5316)" 1))) 
COMMENT                                   "GAL10/CYC1 promoter"))) 
COMMENT                           "Promoter Eukaryotic (1 total)") 
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COMMENT                                     #115=(CLinePar 
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COMMENT                                           "origin plasmid" 1) 
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COMMENT                                           "   complement(6793..7195)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                           "   complement(4491..4595)" 1))) 
COMMENT                                   "FLP terminator"))) "Terminator (1 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N1: 2753 " 1))))) "AflII: 1 site") 
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FEATURES             Location/Qualifiers
     CDS             complement(7735..8595)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(6793..7195)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(5535..6338)
                     /vntifkey="4"
                     /label=URA3
                     /note="S. cerevisiae uracil 3 coding sequence"
     promoter        complement(4909..5316)
                     /vntifkey="29"
                     /label=GAL10/CYC1\promoter
                     /note="UAS-G: 5316-5169, GAL10: 5168-5091,CYC1: 5086-4909, (all 3 compl.)"
     misc_feature    4176..4778
                     /vntifkey="21"
                     /label=2mu\inverted\repeat
                     /note="inverted repeat of 2mu"
     terminator      complement(4491..4595)
                     /vntifkey="43"
                     /label=FLP\terminator
                     /note="S. cerevisiae 2mu FLP terminator"
     rep_origin      complement(3429..4409)
                     /vntifkey="33"
                     /label=2mu\ori
                     /note="2mu origin, incl. region conferring stability (STB)"
     CDS             complement(1977..3071)
                     /vntifkey="4"
                     /label=defective\LEU2
                     /note="defective LEU2 coding sequence"
     rep_origin      complement(754..1209)
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
BASE COUNT     2494 a      1952 c      1893 g      2456 t         5 others
ORIGIN
        1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
      121 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtnta 
      181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata 
      301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag 
      361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta 
      421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt 
      481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa 
      541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga 
      601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga 
      661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc 
      721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa 
      781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
      841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
      901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
      961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
     1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
     1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
     1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
     1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca 
     1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa 
     1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc 
     1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa 
     1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca 
     1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat 
     1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 
     1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 
     1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta 
     1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt 
     1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc 
     1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta 
     1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa 
     1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa 
     2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg 
     2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata 
     2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg 
     2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa 
     2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg 
     2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca 
     2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt 
     2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat 
     2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga 
     2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca 
     2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt 
     2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg 
     2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg 
     2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct 
     2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa 
     2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta 
     3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta 
     3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc 
     3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt 
     3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag 
     3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta 
     3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa 
     3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata 
     3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc 
     3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag 
     3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag 
     3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 
     3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 
     3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 
     3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 
     3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 
     3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 
     3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 
     4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 
     4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 
     4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 
     4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 
     4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg 
     4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc 
     4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac 
     4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 
     4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 
     4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 
     4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 
     4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 
     4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcatgcc tgcaggtcga 
     4801 ctctagagga tccccgggta ccgagctcga attgatccgg taatttagtg tgtgtatttg 
     4861 tgtttgcgtg tctatagaag tatagtaatt tatgctacaa aggacctaat gtataaggaa 
     4921 agaatattta gagaaaagaa gaaaacaaga gttttatata catacagagc acatgcatgc 
     4981 catatgatca tgtgtcgtcg cacacatata tatatgcctg tatgtgtcag cactaaagtt 
     5041 gcctggccat ccacgctata tacacgcctg gcggatctgc tcgaggtcga ttagcctaaa 
     5101 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta 
     5161 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc 
     5221 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa 
     5281 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc 
     5341 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat 
     5401 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatcgggt 
     5461 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact 
     5521 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct 
     5581 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca 
     5641 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc 
     5701 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct 
     5761 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc 
     5821 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac 
     5881 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc 
     5941 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct 
     6001 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat 
     6061 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact 
     6121 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg 
     6181 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca 
     6241 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga 
     6301 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttccggttt 
     6361 ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata 
     6421 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga 
     6481 ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg 
     6541 atgaattgaa ttgaaaagct aattcgtaat catggtcata gctgtttcct gtgtgaaatt 
     6601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 
     6661 gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
     6721 cgggaaacct gtcgtgccan ctggattaat gaatcggcca acgcgcgggg agaggcggtt 
     6781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     6841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     6901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     6961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     7021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     7081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     7141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     7201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     7261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     7321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     7381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     7441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     7501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     7561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     7621 gttagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     7681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     7741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     7801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     7861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     7921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     7981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     8041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     8101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     8161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     8221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     8281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     8341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     8401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     8461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     8521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     8581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     8641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     8701 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 
     8761 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 
//
