LOCUS       pSCGAL10-SN             9906 bp    DNA     circular UNA 30-MAY-2012
DEFINITION  LMBP 2471.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2471
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 30-MAY-2012
            
COMMENT     VNTNAME|pSCGAL10-SN|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pSCGAL10-SN" 0 9906 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "ApaI" "BstXI" "HindIII" "NotI" "PvuI" "XbaI" "AflIII" 
COMMENT         "SfiI" "SmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 9826 9825 1 9906 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 7070 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 2361 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 4779 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 5096 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 1598 0 3195 0 9285 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5909 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AflIII" "acrygt" 2 0 4 2331 0 4855 0 7261 0 
COMMENT            8023 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 1 5895 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 3 5063 0 5081 0 5922 0 0 "")) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 1 754 1209 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(754..1209)") 
COMMENT        #10=(CFSignal (CObList) "defective LEU2" 4 0 1 1977 3071 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1977..3071)") 
COMMENT        #11=(CFSignal (CObList) "2mu ori" 33 0 1 3429 4409 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3429..4409)") 
COMMENT        #12=(CFSignal (CObList) "2mu inverted repeat" 21 0 0 4176 4778 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4176..4778") 
COMMENT        #13=(CFSignal (CObList) "FLP terminator" 43 0 1 4491 4595 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4491..4595)") 
COMMENT        #14=(CFSignal (CObList) "CYC1 terminator" 43 0 1 4781 5051 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4781..5051)") 
COMMENT        #15=(CFSignal (CObList) "3' UTR of hIFN\337" 21 0 1 5114 5313 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5114..5313)") 
COMMENT        #16=(CFSignal (CObList) "hIFN\337" 4 0 1 5314 5877 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5314..5877)") 
COMMENT        #17=(CFSignal (CObList) "GAL10/CYC1 promoter" 29 0 1 6015 6422 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6015..6422)") 
COMMENT        #18=(CFSignal (CObList) "URA3" 4 0 1 6641 7444 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6641..7444)") 
COMMENT        #19=(CFSignal (CObList) "pMB1 ori" 33 0 1 7899 8301 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7899..8301)") 
COMMENT        #20=(CFSignal (CObList) "ampicillin resistance" 4 0 1 8841 9701 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(8841..9701)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #21=(CFSignal (CObList) "LMBP 2471" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "Last modified: 30-MAY-2012" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
COMMENT              #24=(CRefLinePar 
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COMMENT                   "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pSCGAL10-SN'" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 2471." 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/30/12 03:27" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9906 bp" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: UNA" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 30-MAY-2012" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #38=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #39=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 2471") 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 30-MAY-2012"))) 
COMMENT                   "Annotations") 
COMMENT              #44=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1977 (3 #10# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1977  End: 3071 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "defective S. cerevisiae leucine 2 coding sequence" 
COMMENT                                        1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1977..3071)" 1))) 
COMMENT                                 "defective LEU2") 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5314 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5314  End: 5877 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "signal and mature human IFN\337 sequence" 
COMMENT                                        1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5314..5877)" 1))) 
COMMENT                                 "hIFN\337") 
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COMMENT                                  (CParagraph 6641 (3 #18# 0) 1 2 2 0 327) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6641  End: 7444 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae uracil 3 coding sequence" 
COMMENT                                        1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6641..7444)" 1))) "URA3") 
COMMENT                            #61=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8841 (3 #20# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8841  End: 9701 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(8841..9701)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (4 total)") 
COMMENT                     #66=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4176  End: 4778" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "inverted repeat of 2mu" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4176..4778" 1))) 
COMMENT                                 "2mu inverted repeat") 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5114 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5114  End: 5313 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR of the human IFN\337 gene" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5114..5313)" 1))) 
COMMENT                                 "3' UTR of hIFN\337"))) 
COMMENT                          "Misc. Feature (2 total)") 
COMMENT                     #77=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6015 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6015  End: 6422 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "UAS-G: 6422-6275, GAL10: 6274-6193, CYC1: 6192-6015, (all 3 compl.)" 
COMMENT                                        1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6015..6422)" 1))) 
COMMENT                                 "GAL10/CYC1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #83=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 754 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 754   End: 1209 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "phage f1 origin" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(754..1209)" 1))) "f1 ori") 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3429 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3429  End: 4409 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae 2mu plasmid origin, incl. region conferring stability (STB)" 
COMMENT                                        1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3429..4409)" 1))) 
COMMENT                                 "2mu ori") 
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COMMENT                                  (CParagraph 7899 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7899  End: 8301 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7899..8301)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                           (CObjectList 
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COMMENT                                   (CObjectList 
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COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 4491  End: 4595 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "S. cerevisiae 2mu FLP terminator" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(4491..4595)" 1))) 
COMMENT                                  "FLP terminator") 
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COMMENT                                   (CObjectList 
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COMMENT                                          "Start: 4781  End: 5051 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "S. cerevisiae iso-1-cytochrome C terminator" 
COMMENT                                          1) 
COMMENT                                    #108=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(4781..5051)" 1))) 
COMMENT                                  "CYC1 terminator"))) "Terminator (2 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #110=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #111=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #113=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7070 " 1))))) "ApaI: 1 site") 
COMMENT                            5 1 "GGGCCC" "CCCGGG") 
COMMENT                      #114=(CRSFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #116=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2361 " 1))))) "BstXI: 1 site") 
COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                      #117=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 4779 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #120=(CRSFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5096 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #123=(CRSFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5909 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
COMMENT                      #126=(CRSFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #128=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5895 " 1))))) "SfiI: 1 site") 
COMMENT                            8 5 "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
COMMENT                      #129=(CRSFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 1598 " 1) 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3195 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 9285 " 1))))) "PvuI: 3 sites") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #134=(CRSFolderPar 
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FEATURES             Location/Qualifiers
     rep_origin      complement(754..1209)
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     CDS             complement(1977..3071)
                     /vntifkey="4"
                     /label=defective\LEU2
                     /note="defective S. cerevisiae leucine 2 coding sequence"
     rep_origin      complement(3429..4409)
                     /vntifkey="33"
                     /label=2mu\ori
                     /note="S. cerevisiae 2mu plasmid origin, incl. region conferring stability (STB)"
     misc_feature    4176..4778
                     /vntifkey="21"
                     /label=2mu\inverted\repeat
                     /note="inverted repeat of 2mu"
     terminator      complement(4491..4595)
                     /vntifkey="43"
                     /label=FLP\terminator
                     /note="S. cerevisiae 2mu FLP terminator"
     terminator      complement(4781..5051)
                     /vntifkey="43"
                     /label=CYC1\terminator
                     /note="S. cerevisiae iso-1-cytochrome C terminator"
     misc_feature    complement(5114..5313)
                     /vntifkey="21"
                     /label=3'\UTR\of\hIFNß
                     /note="3' UTR of the human IFNß gene"
     CDS             complement(5314..5877)
                     /vntifkey="4"
                     /label=hIFNß
                     /note="signal and mature human IFNß sequence"
     promoter        complement(6015..6422)
                     /vntifkey="29"
                     /label=GAL10/CYC1\promoter
                     /note="UAS-G: 6422-6275, GAL10: 6274-6193, CYC1: 6192-6015, (all 3 compl.)"
     CDS             complement(6641..7444)
                     /vntifkey="4"
                     /label=URA3
                     /note="S. cerevisiae uracil 3 coding sequence"
     rep_origin      complement(7899..8301)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(8841..9701)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     2820 a      2180 c      2126 g      2769 t        11 others
ORIGIN
        1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
      121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta 
      181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata 
      301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag 
      361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta 
      421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt 
      481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa 
      541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga 
      601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga 
      661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc 
      721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa 
      781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
      841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
      901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
      961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
     1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
     1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
     1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
     1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca 
     1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa 
     1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc 
     1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa 
     1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca 
     1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat 
     1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 
     1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 
     1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta 
     1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt 
     1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc 
     1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta 
     1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa 
     1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa 
     2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg 
     2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata 
     2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg 
     2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa 
     2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg 
     2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca 
     2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt 
     2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat 
     2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga 
     2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca 
     2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt 
     2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg 
     2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg 
     2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct 
     2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa 
     2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta 
     3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta 
     3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc 
     3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt 
     3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag 
     3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta 
     3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa 
     3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata 
     3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc 
     3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag 
     3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag 
     3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 
     3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 
     3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 
     3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 
     3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 
     3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 
     3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 
     4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 
     4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 
     4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 
     4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 
     4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg 
     4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc 
     4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac 
     4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 
     4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 
     4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 
     4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 
     4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 
     4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcaaatt aaagccttcg 
     4801 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc 
     4861 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta 
     4921 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag 
     4981 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa 
     5041 aggaaaaggg ggacggatcc ccgggaattc gagctcgccc ggggatcctc taggcggccg 
     5101 ctaggcctcg agggactttt gcaccaaaaa taatttattt tccaaaataa aatttaaata 
     5161 aataaaaata actcataatt taataaaaat ttcaaaatct tctagtgtcc tttcatatgc 
     5221 agtacattag ccatcagtca cttaaacagc atctgctggt tgaagaatgc ttgaagcaat 
     5281 tgtccagtcc cagaggcaca ggctaggaga tcttcagttt cggaggtaac ctgtaagtct 
     5341 gttaatgaag taaaagttcc ttaggatttc cactctgact atggtccagg cacagtgact 
     5401 gtactccttg gccttcaggt aatgcagaat cctcccataa tatcttttca ggtgcagact 
     5461 gctcatgagt tttcccctgg tgaaatcttc tttctccagt ttttcttcca ggactgtctt 
     5521 cagatggttt atctgatgat agacattagc caggaggttc tcaacaatag tctcattcca 
     5581 gccagtgcta gatgaatctt gtctgaaaat agcaaagatg ttctggagca tctcatagat 
     5641 ggtcaatgcg gcgtcctcct tctggaactg ctgcagctgc ttaatctcct cagggatgtc 
     5701 aaagttcatc ctgtccttga ggcagtattc aagcctccca ttcaattgcc acaggagctt 
     5761 ctgacactga aaattgctgc ttctttgtag gaatccaagc aagttgtagc tcatggaaag 
     5821 agctgtagtg gagaagcaca acaggagagc aatttggagg agacacttgt tggtcatgtt 
     5881 cctcgaggcc tttttggcca agctgactct agaggatccc cgggtaccga gctcgaattg 
     5941 atccggtaat ttagtgtgtg tatttgtgtt tgcgtgtcta tagaagtata gtaatttatg 
     6001 ctacaaagga cctaatgtat aaggaaagaa tatttagaga aaagaagaaa acaagagttt 
     6061 tatatacata cagagcacat gcatgccata tgatcatgtg tcgtcgcaca catatatata 
     6121 tgcctgtatg tgtcagcact aaagttgcct ggccatccac gctatataca cgcctggcgg 
     6181 atctgctcga ggtcgattag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct 
     6241 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc 
     6301 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga 
     6361 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt 
     6421 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat 
     6481 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc 
     6541 agcgaagcga tgatttttga tcgggtaata actgatataa ttaaattgaa gctctaattt 
     6601 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc 
     6661 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc 
     6721 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat 
     6781 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg 
     6841 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa 
     6901 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc 
     6961 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct 
     7021 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt 
     7081 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt 
     7141 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg 
     7201 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca 
     7261 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct 
     7321 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa 
     7381 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg 
     7441 acatgattta tcttcgtttc cggtttttgt tctgtgcagt tgggttaaga atactgggca 
     7501 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt 
     7561 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa 
     7621 tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagctaatt cgtaatcatg 
     7681 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     7741 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc 
     7801 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccanctgg attaatgaat 
     7861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     7921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     7981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     8041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     8101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     8161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     8221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     8281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     8341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     8401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     8461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     8521 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     8581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     8641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     8701 tgacgctcag tggaacgaaa actcacgtta gggattttgg tcatgagatt atcaaaaagg 
     8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     9061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     9121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     9181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     9241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     9301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     9361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     9421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     9481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     9541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     9601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     9661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     9721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     9781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 
     9841 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 
     9901 cttcaa 
//
