LOCUS       pBluMTNFR55             5135 bp    DNA     circular      3-MAY-2010
DEFINITION  LMBP 2559.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2559
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 3-MAY-2010
            
            
            
COMMENT     VNTNAME|pBluMTNFR55|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBluMTNFR55" 0 5135 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRI" "ApaI" "ScaI" "NarI" "AccB7I") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5135 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 708 0 2882 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 948 0 2928 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4701 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 1 1389 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AccB7I" "ccannnnntgg" 8 0 1 2189 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "f1 ori" 33 0 0 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4..459") 
COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 0 626 642 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "626..642") 
COMMENT        #7=(CFSignal (CObList) "3' UTR of mTNFR55" 21 0 1 761 1249 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(761..1249)") 
COMMENT        #8=(CFSignal (CObList) "mTNFR55 mature sequence" 4 0 1 1250 2527 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1250..2527)") 
COMMENT        #9=(CFSignal (CObList) "mTNFR55 signal sequence" 4 0 1 2528 2614 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2528..2614)") 
COMMENT        #10=(CFSignal (CObList) "5' UTR of mTNFR55" 21 0 1 2615 2883 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2615..2883)") 
COMMENT        #11=(CFSignal (CObList) "T3 promoter" 30 0 1 2949 2965 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2949..2965)") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 3029 3112 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3029..3112)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 3204 3606 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3204..3606)") 
COMMENT        #14=(CFSignal (CObList) "ampicillin" 4 0 1 4147 5007 0 (CStringList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #16=(CFSignal (CObList) "Last modified: 3-MAY-2010" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pBluMTNFR55'" 1) 
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COMMENT                          1) 
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COMMENT                          "Foreign object. Author: GeneCorner Plasmid Collection. Original author: LMBP Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modified: 05/03/10 12:08" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5135 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Modification Date in the Original DB: 6-MAR-2008" 1))) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Author") 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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COMMENT                                        "Start: 1250  End: 2527 (Complementary)" 
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COMMENT                                   #47=(CLinePar 
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COMMENT                                        "mouse TNF receptor p55 mature sequence" 
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COMMENT                                   #48=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
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COMMENT                                        "mouse TNF receptor p55 signal sequence" 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "3' UTR of the mouse TNF receptor p55 gene" 
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COMMENT                                        "   complement(2615..2883)" 1))) 
COMMENT                                 "5' UTR of mTNFR55"))) 
COMMENT                          "Misc. Feature (2 total)") 
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COMMENT                                        "phage T7 gene 10 promoter" 1) 
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COMMENT                                        "phage T3 promoter" 1) 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                                        "   complement(3029..3112)" 1))) 
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COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     rep_origin      4..459
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     promoter        626..642
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     misc_feature    complement(761..1249)
                     /vntifkey="21"
                     /label=3'\UTR\of\mTNFR55
                     /note="3' UTR of the mouse TNF receptor p55 gene"
     CDS             complement(1250..2527)
                     /vntifkey="4"
                     /label=mTNFR55\mature\sequence
                     /note="mouse TNF receptor p55 mature sequence"
     CDS             complement(2528..2614)
                     /vntifkey="4"
                     /label=mTNFR55\signal\sequence
                     /note="mouse TNF receptor p55 signal sequence"
     misc_feature    complement(2615..2883)
                     /vntifkey="21"
                     /label=5'\UTR\of\mTNFR55
                     /note="5' UTR of the mouse TNF receptor p55 gene"
     promoter        complement(2949..2965)
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="phage T3 promoter"
     promoter        complement(3029..3112)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      complement(3204..3606)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4147..5007)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1189 a      1320 c      1362 g      1264 t 
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tccttttttt 
      721 tttttttttt tttttttttt tttttttttt ttttttttat catttttaga cgtttagtgt 
      781 atttacaaga gtcgagggct ccagctggag accccgcctt tctatgcttg tccttacctt 
      841 acacagttca tccattagtg tgatataatt gatacaaaga ttgcttatca aaagtgaaaa 
      901 aggcccagaa acccactgag gaggccctga gaagcttggc ttgggccctg tgcatcccaa 
      961 gtgggcacca gatacattgt gcctttcctc tacaatcctg tctttggcac cctcagctgg 
     1021 ccaggagctg atggtacatc tccctgccac tcacaagtgg ctggctcagg cagtcctcgg 
     1081 cggctgagaa aagctatgta caccaagtcg gtagcaccaa ggaagtggct accagctcga 
     1141 gccttccccc ttagagacct ttgcccactt ttcacccaca gggagtaggg catctagcag 
     1201 gatggtcctt gaagttcaag tccctcttcc taaggttgtg ggtgtggctt tatcgcggga 
     1261 ggcgggtcgt ggacgagggg gcgggatttc tcagagcctc gaggatattc tccaggcacc 
     1321 cagccaggtt catcttggaa agcacgaggc ccactacttc cagcgtgtcc tcgtggcgcg 
     1381 gcgtgcggcg ccgccaggct tccagcatgc tgtactgagc ctcgcgcagg cagcgcccgt 
     1441 tctgcatctc cagcctctcg atctcgtgct cgctcagccc catgaaacgc atgaactcct 
     1501 tccagcgcgc tggaggcacg ccatccacca cagcatacag aatcgcaagg tctgcattgt 
     1561 caggacgttg cgggtgggcg gagtcttccc atttctgaac agaggtgggg gctggcacgg 
     1621 agcagagtga ttcgtagagc agagggtcag ctccctgggt tgggactacc tcactgacag 
     1681 gtggcatgaa gtgccagtta ctaggaccga agatggggct gatgggggta ctggagacag 
     1741 gagaactaaa gcctggggtg ctgaagccca gagtggggtt gaagccggag gtggggctga 
     1801 aggctgggga gggggctgga gttaggggct ttccagcctt ctcctctttg acaggcacgg 
     1861 gatccctaca aatgatggag tagacttcgg gcctccaccg gggatatcgg cacattaaac 
     1921 tgatgaagat aaaggataga aggcaaagac ctagcaagat aaccaggggc aacagcaccg 
     1981 cagtacctga gtcctggggg tttgtgacat ttgcaagcgg aggaggtagg cacaacttca 
     2041 tacactcctc atttttcttg cagtggctgc aagggacgca ctcactttct ctcagaaaga 
     2101 accctgcatg gcagttacac acggtgttct gagtctcctt acaggggatt gtcacggtgc 
     2161 cgttgaagca ggggctgcag tccacgcact ggaagtgtgt ctcactcagg tagcgttgga 
     2221 actggttctc cttacagcca cacaccgtgt ccttgtcagc ttggcaagga gagatctcca 
     2281 cctgggacat ttctttccga catgtcttgc aactgagaca ctgcctgagg taattctggg 
     2341 aagccgtaaa ggtgcccttt tcacactccc tgcagactgt atcccgccct gggctcggac 
     2401 agtcactcac caagtaggtt cctttgtggc acttggtgca gcagatggaa ttgttcttag 
     2461 aatggacata ctttccttgg ggacacaagc tatccctctt ctcccggtca ccaagagaag 
     2521 ggactagtcc agtgacccct gatggatgta tccccatcag cagagccagg agcaccagtg 
     2581 acagcagcag gccaggcacg gtggggagac ccatgtccgg ccggcagtgg cctctcactc 
     2641 ccccattggg gctggggaca gggcagcaat tgacaacgct cgtgaatgaa gtaagatgat 
     2701 cggaccaggt ggcccgggcg atggcagcct gggcctcgag ttcgagaagc tgaaagtcag 
     2761 aggcagatag agatcagggt acggtcctcc aggacctcgc acccagtagg cccgatcatg 
     2821 agccagagtt cggaaaactc ggagaaaatt taagctgagg ggagagagaa ggtaggagcg 
     2881 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccagctt 
     2941 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 
     3001 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     3061 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     3121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     3181 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     3241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     3301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     3361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     3421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     3481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     3541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     3601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     3661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     3721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     3781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     3841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     3901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     3961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     4021 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     4081 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     4141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     4201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     4261 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     4321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     4381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     4441 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     4501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     4561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     4621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     4681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     4741 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     4801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     4861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     4921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     4981 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     5041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     5101 aggggttccg cgcacatttc cccgaaaagt gccac 
//
