ID pBluMTNFR55 standard; circular DNA; ; 5135 BP. DE LMBP 2559. CC Accession number: LMBP 2559 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 3-MAY-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin 4..459 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 626..642 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT misc_feature complement(761..1249) FT /vntifkey="21" FT /label=3'\UTR\of\mTNFR55 FT /note="3' UTR of the mouse TNF receptor p55 gene" FT CDS complement(1250..2527) FT /vntifkey="4" FT /label=mTNFR55\mature\sequence FT /note="mouse TNF receptor p55 mature sequence" FT CDS complement(2528..2614) FT /vntifkey="4" FT /label=mTNFR55\signal\sequence FT /note="mouse TNF receptor p55 signal sequence" FT misc_feature complement(2615..2883) FT /vntifkey="21" FT /label=5'\UTR\of\mTNFR55 FT /note="5' UTR of the mouse TNF receptor p55 gene" FT promoter complement(2949..2965) FT /vntifkey="30" FT /label=T3\promoter FT /note="phage T3 promoter" FT promoter complement(3029..3112) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin complement(3204..3606) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4147..5007) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 5135 BP; 1189 A; 1320 C; 1362 G; 1264 t; ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 60 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 120 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 180 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 240 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 300 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 360 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 420 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 660 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tccttttttt 720 tttttttttt tttttttttt tttttttttt ttttttttat catttttaga cgtttagtgt 780 atttacaaga gtcgagggct ccagctggag accccgcctt tctatgcttg tccttacctt 840 acacagttca tccattagtg tgatataatt gatacaaaga ttgcttatca aaagtgaaaa 900 aggcccagaa acccactgag gaggccctga gaagcttggc ttgggccctg tgcatcccaa 960 gtgggcacca gatacattgt gcctttcctc tacaatcctg tctttggcac cctcagctgg 1020 ccaggagctg atggtacatc tccctgccac tcacaagtgg ctggctcagg cagtcctcgg 1080 cggctgagaa aagctatgta caccaagtcg gtagcaccaa ggaagtggct accagctcga 1140 gccttccccc ttagagacct ttgcccactt ttcacccaca gggagtaggg catctagcag 1200 gatggtcctt gaagttcaag tccctcttcc taaggttgtg ggtgtggctt tatcgcggga 1260 ggcgggtcgt ggacgagggg gcgggatttc tcagagcctc gaggatattc tccaggcacc 1320 cagccaggtt catcttggaa agcacgaggc ccactacttc cagcgtgtcc tcgtggcgcg 1380 gcgtgcggcg ccgccaggct tccagcatgc tgtactgagc ctcgcgcagg cagcgcccgt 1440 tctgcatctc cagcctctcg atctcgtgct cgctcagccc catgaaacgc atgaactcct 1500 tccagcgcgc tggaggcacg ccatccacca cagcatacag aatcgcaagg tctgcattgt 1560 caggacgttg cgggtgggcg gagtcttccc atttctgaac agaggtgggg gctggcacgg 1620 agcagagtga ttcgtagagc agagggtcag ctccctgggt tgggactacc tcactgacag 1680 gtggcatgaa gtgccagtta ctaggaccga agatggggct gatgggggta ctggagacag 1740 gagaactaaa gcctggggtg ctgaagccca gagtggggtt gaagccggag gtggggctga 1800 aggctgggga gggggctgga gttaggggct ttccagcctt ctcctctttg acaggcacgg 1860 gatccctaca aatgatggag tagacttcgg gcctccaccg gggatatcgg cacattaaac 1920 tgatgaagat aaaggataga aggcaaagac ctagcaagat aaccaggggc aacagcaccg 1980 cagtacctga gtcctggggg tttgtgacat ttgcaagcgg aggaggtagg cacaacttca 2040 tacactcctc atttttcttg cagtggctgc aagggacgca ctcactttct ctcagaaaga 2100 accctgcatg gcagttacac acggtgttct gagtctcctt acaggggatt gtcacggtgc 2160 cgttgaagca ggggctgcag tccacgcact ggaagtgtgt ctcactcagg tagcgttgga 2220 actggttctc cttacagcca cacaccgtgt ccttgtcagc ttggcaagga gagatctcca 2280 cctgggacat ttctttccga catgtcttgc aactgagaca ctgcctgagg taattctggg 2340 aagccgtaaa ggtgcccttt tcacactccc tgcagactgt atcccgccct gggctcggac 2400 agtcactcac caagtaggtt cctttgtggc acttggtgca gcagatggaa ttgttcttag 2460 aatggacata ctttccttgg ggacacaagc tatccctctt ctcccggtca ccaagagaag 2520 ggactagtcc agtgacccct gatggatgta tccccatcag cagagccagg agcaccagtg 2580 acagcagcag gccaggcacg gtggggagac ccatgtccgg ccggcagtgg cctctcactc 2640 ccccattggg gctggggaca gggcagcaat tgacaacgct cgtgaatgaa gtaagatgat 2700 cggaccaggt ggcccgggcg atggcagcct gggcctcgag ttcgagaagc tgaaagtcag 2760 aggcagatag agatcagggt acggtcctcc aggacctcgc acccagtagg cccgatcatg 2820 agccagagtt cggaaaactc ggagaaaatt taagctgagg ggagagagaa ggtaggagcg 2880 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccagctt 2940 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 3000 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 3060 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 3120 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 3180 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 3240 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 3300 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 3360 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3420 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3480 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3540 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 3600 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3660 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 3720 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 3780 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 3840 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 3900 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 3960 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 4020 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 4080 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 4140 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 4200 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 4260 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 4320 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 4380 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 4440 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 4500 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 4560 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 4620 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 4680 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 4740 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 4800 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 4860 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 4920 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 4980 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 5040 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 5100 aggggttccg cgcacatttc cccgaaaagt gccac 5135 //