LOCUS       pATTrpMTNFm29S32W        3970 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2658.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATTrpMTNFm29S32W|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATTrpMTNFm29S32W" 0 3970 
COMMENT       (CDBMol 0 0 1 1 1 0 0 28120184 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3970 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "5UTR" 21 0 0 14 42 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "14..42") 
COMMENT        #4=(CFSignal (CObList) "mTNFm" 4 0 0 43 516 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "43..516") 
COMMENT        #5=(CFSignal (CObList) "3UTR" 21 0 0 517 764 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "517..764") 
COMMENT        #6=(CFSignal (CObList) "TET" 4 0 0 898 2088 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "898..2088") 
COMMENT        #7=(CFSignal (CObList) "ORI" 33 0 1 2460 2860 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2460..2860)") 
COMMENT        #8=(CFSignal (CObList) "trp" 30 0 0 3728 13 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3728..13")) (CObList) (CObList) (CObList) 
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COMMENT       (CTextView 0 
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COMMENT                         "DNA 'pATTrpMTNFm29S32W'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
COMMENT                    #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Creation Date: ? (no data)" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3970 bp" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
COMMENT                    #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                                       "Start: 43    End: 516 " 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "mutated mouse TNF mature sequence" 1) 
COMMENT                                  #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #30=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   43..516" 1))) "mTNFm") 
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COMMENT                                       "Start: 898   End: 2088" 1) 
COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #35=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   898..2088" 1))) "TET"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                  #39=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "5' UTR, incl. ribosome binding site trp operon" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "Start: 517   End: 764 " 1) 
COMMENT                                  #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of the mouse tumor necrosis factor gene" 
COMMENT                                       1) 
COMMENT                                  #45=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #46=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   517..764" 1))) "3UTR"))) 
COMMENT                         "Misc. Feature (2 total)") 
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COMMENT                                  #50=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "E. coli tryptophan operon promoter" 1) 
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COMMENT                         "Promoter Prokaryotic (1 total)") 
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COMMENT                                       "Start: 2460  End: 2860 (Complementary)" 
COMMENT                                       1) 
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COMMENT                                       "origin plasmid" 1) 
COMMENT                                  #57=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
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FEATURES             Location/Qualifiers
     misc_feature    14..42
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR, incl. ribosome binding site trp operon"
     CDS             43..516
                     /vntifkey="4"
                     /label=mTNFm
                     /note="mutated mouse TNF mature sequence"
     misc_feature    517..764
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the mouse tumor necrosis factor gene"
     CDS             898..2088
                     /vntifkey="4"
                     /label=TET
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(2460..2860)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     promoter        3728..13
                     /vntifkey="30"
                     /label=trp
                     /note="E. coli tryptophan operon promoter"
BASE COUNT      853 a      1124 c      1069 g       918 t         6 others
ORIGIN
        1 gttaactagt acgcaagttc acgtaaaaag ggtatcgatt ccatggtaag atcaagtagt 
       61 caaaattcga gtgacaagcc tgtagcccac gtcgtagcaa accaccaagt ggaggagcag 
      121 ctggagtggt cgagccagtg ggccaacgcc ctcctggcca acggcatgga tctcaaagac 
      181 aaccaactag tggtgccagc cgatgggttg taccttgtct actcccaggt tctcttcaag 
      241 ggacaaggct gccccgacta cgtgctcctc acccacaccg tcagccgatt tgtgatctca 
      301 taccaggaga aagtcaacct cctctctgcc gtcaagagcc cctgccccaa ggacacccct 
      361 gagggggctg agctcaaacc ctggtatgag cccatatacc tgggaggagt cttccagctg 
      421 gagaaggggg accaactcag cgctgaggtc aatctgccca agtacttaga ctttgcggag 
      481 tccgggcagg tctactttgg agtcattgct ctgtgaaggg aatgggtgtt catccattct 
      541 ctacccagcc cccactctga cccctttact ctgacccctt tattgtctac tcctcagagc 
      601 ccccagtctg tgtccttcta acttagaaag gggattatgg ctcagagtcc aactctgtgc 
      661 tcagagcttt caacaactac tcagaaacac aagatgctgg gacagtgacc tggactgtgg 
      721 gcctctcatg caccaccatc aaggactcaa atgggctttc cgaattctag agcttgcacc 
      781 caaggggggg ggggggggga gggggggatc aattctcatg tttgacagct tatcatcgat 
      841 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg 
      901 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata 
      961 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc 
     1021 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc 
     1081 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt 
     1141 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat cctctacgcc 
     1201 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 
     1261 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 
     1321 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 
     1381 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 
     1441 caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc 
     1501 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc 
     1561 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt 
     1621 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc 
     1681 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc 
     1741 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg 
     1801 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag 
     1861 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg 
     1921 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc 
     1981 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc 
     2041 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc 
     2101 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa 
     2161 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca 
     2221 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc 
     2281 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat 
     2341 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa 
     2401 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc 
     2461 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     2521 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     2581 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     2641 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     2701 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     2761 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     2821 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     2881 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     2941 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     3001 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     3061 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     3121 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     3181 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     3241 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     3301 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     3361 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     3421 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     3481 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     3541 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     3601 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     3661 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca 
     3721 gnnnnnnctg tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca 
     3781 cggtgcacca ttgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg 
     3841 tacgtaaatc actgcataat tcgtgtcgtc caaggcgcac tcccgttctg gataatgttt 
     3901 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat 
     3961 catcgaacta 
//
