LOCUS       pATMxH5HT2R             6074 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2783.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pATMxH5HT2R|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATMxH5HT2R" 0 6074 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6074 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "Mx" 30 0 0 41 486 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "41..486") 
COMMENT        #4=(CFSignal (CObList) "5HT2R" 4 0 0 502 1917 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "502..1917") 
COMMENT        #5=(CFSignal (CObList) "(t)" 4 0 0 1945 2019 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1945..2019") 
COMMENT        #6=(CFSignal (CObList) "INT" 15 0 0 2020 2086 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "3UTR" 21 0 0 2087 2638 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2087..2638") 
COMMENT        #8=(CFSignal (CObList) "polyA" 43 0 0 2639 2735 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2639..2735") 
COMMENT        #9=(CFSignal (CObList) "polyA" 43 0 1 2639 2735 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2639..2735)") 
COMMENT        #10=(CFSignal (CObList) "ORI" 33 0 1 4063 4465 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4063..4465)") 
COMMENT        #11=(CFSignal (CObList) "AMP" 4 0 1 5006 5866 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5006..5866)")) (CObList) 
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COMMENT                          "DNA 'pATMxH5HT2R'" 1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6074 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 502   End: 1917" 1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human serotonin 5HT2 receptor gene" 1) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #32=(CLinePar 
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COMMENT                                        "   502..1917" 1))) "5HT2R") 
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COMMENT                                        "Start: 1945  End: 2019" 1) 
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COMMENT                                        "part of the SV40 small t-antigen" 1) 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "Start: 2020  End: 2086" 1) 
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COMMENT                                        "intron of the SV40 small t-antigen" 1) 
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COMMENT                                        "3' UTR of the SV40 small t-antigen" 1) 
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COMMENT                                        "mouse Mx promoter" 1) 
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FEATURES             Location/Qualifiers
     promoter        41..486
                     /vntifkey="30"
                     /label=Mx
                     /note="mouse Mx promoter"
     CDS             502..1917
                     /vntifkey="4"
                     /label=5HT2R
                     /note="human serotonin 5HT2 receptor gene"
     CDS             1945..2019
                     /vntifkey="4"
                     /label=(t)
                     /note="part of the SV40 small t-antigen"
     intron          2020..2086
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the SV40 small t-antigen"
     misc_feature    2087..2638
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the SV40 small t-antigen"
     terminator      2639..2735
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2639..2735)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(4063..4465)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(5006..5866)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1505 a      1517 c      1463 g      1589 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gcttgggctg cagtagcagt gctcagacct 
       61 ctaaacctgt agtcacaagg gacccagaag ggctggcagg gctcctcagt gattctggta 
      121 tggtttgtct ctgacccttc ctagtgcagc tggaagaatc cagtgaaaag agaaagttag 
      181 tctaggttgt gttcaactga gaggaggtca ggctgaaggc tcagcacaga gctggaaggg 
      241 tcctgggaaa gcaggcagcc atctgaactg gtgtccatac tgcatgtgtc ttggtgggct 
      301 gagcctacaa tatcgatttc ccaacctcag taccaagcca aggcccctca gggtgagttt 
      361 cgtttctgag ctcctttcat atgtggtggg ggtgtgggca cagcaagctc aggctttttc 
      421 acggtttcaa ttctcctctg gaaggggcgg agccagcact atttaaagag gtctgagccc 
      481 tggcaggact ctagcaggcc tatggatatt ctttgtgaag aaaatacttc tttgagctca 
      541 actacgaact ccctaatgca attaaatgat gacaccaggc tctacagtaa tgactttaac 
      601 tctggagaag ctaacacttc tgatgcattt aactggacag tcgactctga aaatcgaacc 
      661 aacctttcct gtgaagggtg cctctcaccg tcgtgtctct ccttacttca tctccaggaa 
      721 aaaaactggt ctgctttact gacagccgta gtgattattc taactattgc tggaaacata 
      781 ctcgtcatca tggcagtgtc cctagagaaa aagctgcaga atgccaccaa ctatttcctg 
      841 atgtcacttg ccatagctga tatgctgctg ggtttccttg tcatgcccgt gtccatgtta 
      901 accatcctgt atgggtaccg gtggcctctg ccgagcaagc tttgtgcagt ctggatttac 
      961 ctggacgtgc tcttctccac ggcctccatc atgcacctct gcgccatctc gctggaccgc 
     1021 tacgtcgcca tccagaatcc catccaccac agccgcttca actccagaac taaggcattt 
     1081 ctgaaaatca ttgctgtttg gaccatatca gtaggtatat ccatgccaat accagtcttt 
     1141 gggctacagg acgattcgaa ggtctttaag gaggggagtt gcttactcgc cgatgataac 
     1201 tttgtcctga tcggctcttt tgtgtcattt ttcattccct taaccatcat ggtgatcacc 
     1261 tactttctaa ctatcaagtc actccagaaa gaagctactt tgtgtgtaag tgatcttggc 
     1321 acacgggcca aattagcttc tttcagcttc ctccctcaga gttctttgtc ttcagaaaag 
     1381 ctcttccagc ggtcgatcca tagggagcca gggtcctaca caggcaggag gactatgcag 
     1441 tccatcagca atgagcaaaa ggcatgcaag gtgctgggca tcgtcttctt cctgtttgtg 
     1501 gtgatgtggt gccctttctt catcacaaac atcatggccg tcatctgcaa agagtcctgc 
     1561 aatgaggatg tcattggggc cctgctcaat gtgtttgttt ggatcggtta tctctcttca 
     1621 gcagtcaacc cactagtcta cacactgttc aacaagacct ataggtcagc cttttcacgg 
     1681 tatattcagt gtcagtacaa ggaaaacaaa aaaccattgc agttaatttt agtgaacaca 
     1741 ataccggctt tggcctacaa gtctagccaa cttcaaatgg gacaaaaaaa gaattcaaag 
     1801 caagatgcca agacaacaga taatgactgc tcaatggttg ctctaggaaa gcagcattct 
     1861 gaagaggctt ctaaagacaa tagcgacgga gtgaatgaaa aggtgagctg tgtgtgactc 
     1921 gagggggatc ctctagagtc gacggatctt tgtgaaggaa ccttacttct gtggtgtgac 
     1981 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag 
     2041 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg 
     2101 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga 
     2161 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa 
     2221 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag 
     2281 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa 
     2341 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca 
     2401 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc 
     2461 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa 
     2521 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg 
     2581 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 
     2641 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 
     2701 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     2761 ccaaactcat caatgtatct tatcatgtct ggatcctcta cgccggacgc atcgtggccg 
     2821 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg 
     2881 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag 
     2941 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg 
     3001 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg 
     3061 gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc 
     3121 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac 
     3181 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga 
     3241 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca 
     3301 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg 
     3361 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta 
     3421 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc 
     3481 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa 
     3541 cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga 
     3601 acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc 
     3661 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg 
     3721 attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac 
     3781 caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat 
     3841 ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac 
     3901 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg 
     3961 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt 
     4021 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc gcttcctcgc 
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     4921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     4981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     5041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     5101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     5161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     5221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     5281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac 
     5341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     5401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     5461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     5521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     5581 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 
     5641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     5701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     5761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     5821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     5881 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     5941 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     6001 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 
     6061 ttcgtcttca agaa 
//
