LOCUS       pSP64GSHIFNBNT          4417 bp    DNA     circular      7-APR-2008
DEFINITION  LMBP 2789.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2789
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 7-APR-2008
            
COMMENT     VNTDATE|454950036|
COMMENT     VNTNAME|pSP64GSHIFNBNT|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pSP64GSHIFNBNT" 0 4417 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 0 0 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "Alw44I" "Eco81I" "HindIII" "PstI" "XhoI" "SfiI" "StuI" 
COMMENT         "NotI" "XbaI" "BglI" "Eco130I") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4417 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 63 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 296 0 1454 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 81 0 856 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 2135 0 3381 0 3878 0 0 
COMMENT            "") 
COMMENT        #4=(CRSite (CStringList) "Eco81I" "cctnagg" 3 0 2 275 0 604 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 1 76 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "StuI" "aggcct" 4 0 2 79 0 862 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 868 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 2 76 0 2834 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "Eco130I" "ccwwgg" 2 0 1 557 0 0 "")) 
COMMENT       (CObList 
COMMENT        #11=(CFSignal (CObList) "5' UTR of \337-globin" 21 0 0 11 53 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "11..53") 
COMMENT        #12=(CFSignal (CObList) "3' UTR of \337-globin" 21 0 0 910 1050 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "910..1050") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 1 1522 1605 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1522..1605)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 1697 2099 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1700..2100)") 
COMMENT        #15=(CFSignal (CObList) "ampicillin" 4 0 1 2640 3500 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2640..3500)") 
COMMENT        #16=(CFSignal (CObList) "SP6 promoter" 30 0 0 4395 4411 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4395..4411") 
COMMENT        #17=(CFSignal (CObList) "hIFN\337" 4 0 0 90 653 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "90..653") 
COMMENT        #18=(CFSignal (CObList) "3' UTR of hIFN\337" 21 0 0 654 853 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "654..853") 
COMMENT        #19=(CFSignal (CObList) "hIFNalpha14 terminator" 43 0 0 1114 1445 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1114..1445")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 2789" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 7-APR-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSP64GSHIFNBNT" 2) 
COMMENT                   5 "" 0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pSP64GSHIFNBNT'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 2789" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/17/07 03:00" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/07/08 02:05" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4417 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 16-JAN-2008" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #36=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #39=(CLinePar (CParagraph 0 (15 #20# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 2789") 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 7-APR-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 90 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 90    End: 653 " 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   90..653" 1))) "hIFN\337") 
COMMENT                            #48=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2640 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2640  End: 3500 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2640..3500)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #52=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 11 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 11    End: 53  " 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   11..53" 1))) "5' UTR of \337-globin") 
COMMENT                            #57=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 654 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 654   End: 853 " 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   654..853" 1))) "3' UTR of hIFN\337") 
COMMENT                            #61=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 910 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 910   End: 1050" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   910..1050" 1))) 
COMMENT                                 "3' UTR of \337-globin"))) 
COMMENT                          "Misc. Feature (3 total)") 
COMMENT                     #65=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1522 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1522  End: 1605 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1522..1605)" 1))) 
COMMENT                                 "lac promoter") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4395 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4395  End: 4411" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4395..4411" 1))) "SP6 promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #74=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1697 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1697  End: 2099 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1700..2100)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (1 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 1 178) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1114 (3 #19# 0) 1 2 2 1 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1114  End: 1445" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human interferon alpha 14 gene terminator" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1114..1445" 1))) 
COMMENT                                 "hIFNalpha14 terminator"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map") 
COMMENT              #85=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #86=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 0 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "XbaI: 0 sites") 1 5 "TCTAGA" 
COMMENT                          "AGATCT") 
COMMENT                     #87=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #88=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 63 " 1))))) "HindIII: 1 site") 1 5 
COMMENT                          "AAGCTT" "TTCGAA") 
COMMENT                     #90=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #91=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 76 " 1))))) "SfiI: 1 site") 8 5 
COMMENT                          "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
COMMENT                     #93=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #94=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 868 " 1))))) "NotI: 1 site") 2 6 
COMMENT                          "GCGGCCGC" "CGCCGGCG") 
COMMENT                     #96=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #97=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 557 " 1))))) "Eco130I: 1 site") 1 
COMMENT                          5 "CCWWGG" "GGWWCC") 
COMMENT                     #99=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #100=(CGroupPar 
COMMENT                                   (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                          " N1: 296 " 1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                          " N2: 1454 " 1))))) "PstI: 2 sites") 5 
COMMENT                          1 "CTGCAG" "GACGTC") 
COMMENT                     #103=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #104=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 81 " 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 856 " 1))))) "XhoI: 2 sites") 1 
COMMENT                           5 "CTCGAG" "GAGCTC") 
COMMENT                     #107=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #108=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 275 " 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 604 " 1))))) "Eco81I: 2 sites") 
COMMENT                           2 5 "CCTNAGG" "GGANTCC") 
COMMENT                     #111=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #112=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 79 " 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 862 " 1))))) "StuI: 2 sites") 3 
COMMENT                           3 "AGGCCT" "TCCGGA") 
COMMENT                     #115=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #116=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 76 " 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 2834 " 1))))) "BglI: 2 sites") 
COMMENT                           7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
COMMENT                     #119=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #120=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2135 " 1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3381 " 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 3878 " 1))))) "Alw44I: 3 sites") 
COMMENT                           1 5 "GTGCAC" "CACGTG"))) 
COMMENT                   "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #124=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #125=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #126=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #127=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #128=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #129=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #130=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #131=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #132=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #133=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #134=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #135=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #136=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #137=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #138=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #139=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #140=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #141=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #142=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #143=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #144=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #145=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #146=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #147=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #148=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #149=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #150=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #151=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #152=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #153=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #154=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #155=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #156=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #157=(CWidgetStyle "~Scrap" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #158=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #159=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #160=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #161=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #162=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #163=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #164=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #165=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #166=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #167=(CWidgetStyle "3'UTR Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #168=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #169=(CWidgetStyle "Enhancer Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #170=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #171=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #172=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #173=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #174=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #175=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #176=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #177=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #178=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #179=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #180=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #181=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #182=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #183=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #184=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #185=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #186=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #187=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #188=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #189=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #190=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #191=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #192=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #193=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #194=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #195=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #196=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #197=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #198=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #200=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #201=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #202=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #203=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #204=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #205=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #206=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #207=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #208=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #209=(CWidgetStyle "Attenuator" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #210=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6684774) 4 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #211=(CWidgetStyle "Promoter_P" 1 (LOGPEN 0 0 6684774) 8 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #212=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 6684774) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #213=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 0) 7 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #214=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #215=(CWidgetStyle "Label 24" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #216=(CWidgetStyle "Label 25" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #217=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #218=(CWidgetStyle "Attenuator Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #219=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 6723891) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #220=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 6723891) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #221=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 3368448) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #222=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 6723891 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #223=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 52479 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #224=(CWidgetStyle "Shape 24" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 3381759 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #225=(CWidgetStyle "Shape 25" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 10066329 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #226=(CWidgetStyle "Shape 26" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 52275 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #227=(CWidgetStyle "Shape 27" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16763904 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #228=(CWidgetStyle "Shape 28" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 13395456 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #229=(CWidgetStyle "Shape 29" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 13408563 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #230=(CWidgetStyle "Shape 30" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 30" 1 "Attenuator Label") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 20") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 20") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 20") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Signal Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 9" 1 "Label 16") 68 
COMMENT          (CShapeMapEntry 0 "Shape 8" 1 "Label 23") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 44.1479 44.1479 39 39 0.1 
COMMENT         -4417) 1 1 0 1 1 
COMMENT        (mapper: 19.2971 -22.8878 44.1479 44.1479 0.01 10 -1 4417 4417 1 0 0) 
COMMENT        #231=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -3041 100) 
COMMENT              (CObjectList 
COMMENT               #232=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1145 100) 
COMMENT                     (CObjectList 
COMMENT                      #233=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #140# 87081836 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27561 
COMMENT                             6.27361 1 0.0214037) 0.142024) 
COMMENT                      #234=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #134# 31806036 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 69 27 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pSP64GSHIFNBNT" "@N" 1 0.0679534 
COMMENT                            1.86781 0 -10 12.5291 1.57175 Nil) 
COMMENT                      #235=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #133# 18088512 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 80 32 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "4417 bp" "@L bp" 16 
COMMENT                            0.0679534 0.193438 0 -10.8 5.3215 1.81874 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #236=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1089 100) 
COMMENT                     (CObjectList 
COMMENT                      #237=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -1376 100) 
COMMENT                            (CObjectList 
COMMENT                             #238=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #136# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    1.30236 2.52518 1 0.082322) 0.8 1.8 1) 
COMMENT                             #239=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 87060828 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ampicillin" 
COMMENT                                   "@N" 1 -13.6571 -4.79662 0 -10 4.01919 
COMMENT                                   1.14513 #238#) 
COMMENT                             #240=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #136# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.34577 6.14678 1 0.082322) 0.8 1.8 0) 
COMMENT                             #241=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 87061180 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "hIFN\337" "@N" 
COMMENT                                   1 7.1905 10.6877 0 -10 2.69443 1.14513 #240#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #242=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -617 100) 
COMMENT                            (CObjectList 
COMMENT                             #243=(CLine 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #144# 
COMMENT                                    1816181360 100) (LOGPEN 0 6 16711680) 8 
COMMENT                                   0.835356 6.19791 6.25898) 
COMMENT                             #244=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 28157316 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of \337-globin" "@N" 1 5.03437 
COMMENT                                   19.4619 0 -10 7.90367 1.14513 #243#) 
COMMENT                             #245=(CLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #144# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 4.78194 
COMMENT                                   4.98219) 
COMMENT                             #246=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 18088504 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' UTR of \337-globin" "@N" 1 15.3668 
COMMENT                                   2.04764 0 -10 7.90367 1.14513 #245#) 
COMMENT                             #247=(CLine 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #144# -981 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 5.06172 
COMMENT                                   5.34577) 
COMMENT                             #248=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 87059772 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' UTR of hIFN\337" "@N" 1 13.2613 6.75185 0 
COMMENT                                   -10 7.05043 1.14513 #247#)) (CObjectList)) 
COMMENT                      #249=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -945 100) 
COMMENT                            (CObjectList 
COMMENT                             #250=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #214# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    3.99371 4.11301 1 0.082322) 0.8 1.8 1) 
COMMENT                             #251=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 87061884 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 11.8173 -7.77753 0 -10 5.47868 1.14513 
COMMENT                                   #250#) 
COMMENT                             #252=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #146# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 6.22858 0.0326654) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #253=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 87060476 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SP6 promoter" 
COMMENT                                   "@N" 1 3.26479 20.7286 0 -10 6.08492 1.14513 
COMMENT                                   #252#)) (CObjectList)) 
COMMENT                      #254=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -1582 100) 
COMMENT                            (CObjectList 
COMMENT                             #255=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #135# 1110 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.29211 3.86446 1 0.082322) 0.8 1.8 1) 
COMMENT                             #256=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 87061532 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 7.12318 -11.717 0 -10 3.92938 1.14513 #255#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #257=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -313 100) 
COMMENT                            (CObjectList 
COMMENT                             #258=(CLine 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #144# -272 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 4.22094 
COMMENT                                   4.69246) 
COMMENT                             #259=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 87060124 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 51 20 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hIFNalpha14 terminator" "@N" 1 15.6457 
COMMENT                                   -3.36418 0 -10 10.3287 1.14513 #258#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #260=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -828 100) 
COMMENT                     (CObjectList 
COMMENT                      #261=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -364 100) 
COMMENT                            (CObjectList 
COMMENT                             #262=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #194# 337 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 6.18513 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #263=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #193# 87058716 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "HindIII (63)" "@N (@S)" 5 4.02251 18.2179 0 
COMMENT                                   -10 4.73771 1.05532 #262#)) (CObjectList)) 
COMMENT                      #264=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1276 100) 
COMMENT                            (CObjectList 
COMMENT                             #265=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #194# 18088392 
COMMENT                                    100) (LOGPEN 0 0 16737792) 1 1 6.16667 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #266=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #193# 87015580 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "SfiI (76)" "@N (@S)" 5 3.50395 17.2004 0 -10 
COMMENT                                   3.30068 1.05532 #265#)) (CObjectList)) 
COMMENT                      #267=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -620 100) 
COMMENT                            (CObjectList 
COMMENT                             #268=(CScratch 
COMMENT                                   (CWidget 1 (1 #7# 1) 1 2 0 0 #194# 402 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 5.04184 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #269=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #193# 87014108 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "NotI (868)" "@N (@S)" 5 13.5596 3.15574 0 
COMMENT                                   -10 4.10901 1.05532 #268#)) (CObjectList)) 
COMMENT                      #270=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #10# 0) 1 2 0 0 Nil -268 100) 
COMMENT                            (CObjectList 
COMMENT                             #271=(CScratch 
COMMENT                                   (CWidget 1 (1 #10# 1) 1 2 0 0 #194# 28254436 
COMMENT                                    100) (LOGPEN 0 0 16737792) 1 1 5.48353 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #272=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #193# 87014876 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "Eco130I (557)" "@N (@S)" 5 11.3813 9.05865 0 
COMMENT                                   -10 5.56849 1.05532 #271#)) (CObjectList)) 
COMMENT                      #273=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -666 100) 
COMMENT                            (CObjectList 
COMMENT                             #274=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.85422 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #275=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87059068 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "PstI (296)" "@N (@S)" 5 7.17794 11.8411 0 
COMMENT                                   -10 3.81711 1.05532 #274#) 
COMMENT                             #276=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #192# 31112104 
COMMENT                                    100) (LOGPEN 0 0 3381504) 1 1 4.20958 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #277=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87059420 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "PstI (1454)" "@N (@S)" 5 12.187 -5.96725 0 
COMMENT                                   -10 4.31109 1.05532 #276#)) (CObjectList)) 
COMMENT                      #278=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #2# 0) 1 2 0 0 Nil -455 100) 
COMMENT                            (CObjectList 
COMMENT                             #279=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 6.15957 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #280=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87057660 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "XhoI (81)" "@N (@S)" 5 4.45812 14.0347 0 -10 
COMMENT                                   3.81711 1.05532 #279#) 
COMMENT                             #281=(CScratch 
COMMENT                                   (CWidget 2 (1 #2# 2) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.05888 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #282=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87058012 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "XhoI (856)" "@N (@S)" 5 13.43 5.50784 0 -10 
COMMENT                                   4.31109 1.05532 #281#)) (CObjectList)) 
COMMENT                      #283=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -411 100) 
COMMENT                            (CObjectList 
COMMENT                             #284=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.88404 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #285=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87056956 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "Eco81I (275)" "@N (@S)" 5 7.50648 12.9266 0 
COMMENT                                   -10 5.09697 1.05532 #284#) 
COMMENT                             #286=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #192# 298 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.41678 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #287=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87057308 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "Eco81I (604)" "@N (@S)" 5 11.3666 7.95055 0 
COMMENT                                   -10 5.09697 1.05532 #286#)) (CObjectList)) 
COMMENT                      #288=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -503 100) 
COMMENT                            (CObjectList 
COMMENT                             #289=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 6.16241 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #290=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87015932 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "StuI (79)" "@N (@S)" 5 4.11166 15.0522 0 -10 
COMMENT                                   3.4354 1.05532 #289#) 
COMMENT                             #291=(CScratch 
COMMENT                                   (CWidget 2 (1 #6# 2) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.05036 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #292=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87055900 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "StuI (862)" "@N (@S)" 5 13.366 4.4677 0 -10 
COMMENT                                   3.92938 1.05532 #291#)) (CObjectList)) 
COMMENT                      #293=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #9# 0) 1 2 0 0 Nil -883 100) 
COMMENT                            (CObjectList 
COMMENT                             #294=(CScratch 
COMMENT                                   (CWidget 1 (1 #9# 1) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 6.16667 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #295=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87013724 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "BglI (76)" "@N (@S)" 5 3.92266 16.1603 0 -10 
COMMENT                                   3.57012 1.05532 #294#) 
COMMENT                             #296=(CScratch 
COMMENT                                   (CWidget 2 (1 #9# 2) 1 2 0 0 #192# 31889300 
COMMENT                                    100) (LOGPEN 0 0 3381504) 1 1 2.24965 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #297=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87014492 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "BglI (2834)" "@N (@S)" 5 -11.9021 -8.14537 0 
COMMENT                                   -10 4.51317 1.05532 #296#)) (CObjectList)) 
COMMENT                      #298=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #3# 0) 1 2 0 0 Nil -786 100) 
COMMENT                            (CObjectList 
COMMENT                             #299=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #192# 18088408 
COMMENT                                    100) (LOGPEN 0 0 3381504) 1 1 3.2424 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #300=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87058364 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "Alw44I (2135)" "@N (@S)" 5 4.06514 -13.1875 
COMMENT                                   0 -10 5.74812 1.05532 #299#) 
COMMENT                             #301=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #192# 18088392 
COMMENT                                    100) (LOGPEN 0 0 3381504) 1 1 1.47279 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #302=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87056604 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "Alw44I (3381)" "@N (@S)" 5 -14.8764 1.74233 
COMMENT                                   0 -10 5.74812 1.05532 #301#) 
COMMENT                             #303=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #192# 18088392 
COMMENT                                    100) (LOGPEN 0 0 3381504) 1 1 0.766928 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #304=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 87056252 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "Alw44I (3878)" "@N (@S)" 5 -11.0286 9.02696 
COMMENT                                   0 -10 5.74812 1.05532 #303#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #305=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -222 100) 
COMMENT                     (CObjectList 
COMMENT                      #306=(CLabel 
COMMENT                            (CWidget 0 (15 #20# 0) 1 2 0 0 #124# 87015228 100) 
COMMENT                            (LOGPEN 0 87015240 16724787) 87015252 
COMMENT                            (LOGFONT 69 27 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 2789" 
COMMENT                            "LMBP 2789" 65535 0.339767 -1.56293 0 -10 7.61177 
COMMENT                            1.57175 Nil) 
COMMENT                      #307=(CLabel 
COMMENT                            (CWidget 0 (15 #21# 0) 1 2 0 0 #124# 87012572 100) 
COMMENT                            (LOGPEN 268747840 87012584 268747872) 87012596 
COMMENT                            (LOGFONT 46 18 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 7-APR-2008" 
COMMENT                            "Last modified: 7-APR-2008" 65535 0.20386 -3.03525 0 
COMMENT                            -10 10.9798 1.05532 Nil)) (CObjectList)) 
COMMENT               #308=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1160 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 2085770325 
COMMENT       (CStringList) 539440508 542266453 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    11..53
                     /vntifkey="21"
                     /label=5'\UTR\of\ß-globin
                     /note="5' UTR of the X. laevis ß-globin gene"
     CDS             90..653
                     /vntifkey="4"
                     /label=hIFNß
                     /note="human interferon ß signal and mature sequence (signal seq.: 90-152)"
     misc_feature    654..853
                     /vntifkey="21"
                     /label=3'\UTR\of\hIFNß
     misc_feature    910..1050
                     /vntifkey="21"
                     /label=3'\UTR\of\ß-globin
                     /note="3' UTR of the X. laevis ß-globin gene"
     terminator      1114..1445
                     /vntifkey="43"
                     /label=hIFNalpha14\terminator
                     /note="human interferon alpha 14 gene terminator"
     promoter        complement(1522..1605)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(1697..2099)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2640..3500)
                     /vntifkey="4"
                     /label=ampicillin
     promoter        4395..4411
                     /vntifkey="30"
                     /label=SP6\promoter
BASE COUNT     1185 a      1094 c      1009 g      1129 t 
ORIGIN
        1 aagctagctt cttgttcttt ttgcagaagc tcagaataaa cgctcaactt tggcagatcc 
       61 caagcttggc caaaaaggcc tcgaggaaca tgaccaacaa gtgtctcctc caaattgctc 
      121 tcctgttgtg cttctccact acagctcttt ccatgagcta caacttgctt ggattcctac 
      181 aaagaagcag caattttcag tgtcagaagc tcctgtggca attgaatggg aggcttgaat 
      241 actgcctcaa ggacaggatg aactttgaca tccctgagga gattaagcag ctgcagcagt 
      301 tccagaagga ggacgccgca ttgaccatct atgagatgct ccagaacatc tttgctattt 
      361 tcagacaaga ttcatctagc actggctgga atgagactat tgttgagaac ctcctggcta 
      421 atgtctatca tcagataaac catctgaaga cagtcctgga agaaaaactg gagaaagaag 
      481 atttcaccag gggaaaactc atgagcagtc tgcacctgaa aagatattat gggaggattc 
      541 tgcattacct gaaggccaag gagtacagtc actgtgcctg gaccatagtc agagtggaaa 
      601 tcctaaggaa cttttacttc attaacagac ttacaggtta cctccgaaac tgaagatctc 
      661 ctagcctgtg cctctgggac tggacaattg cttcaagcat tcttcaacca gcagatgctg 
      721 tttaagtgac tgatggctaa tgtactgcat atgaaaggac actagaagat tttgaaattt 
      781 ttattaaatt atgagttatt tttatttatt taaattttat tttggaaaat aaattatttt 
      841 tggtgcaaaa gtccctcgag gcctagcggc cgcctagagg atccccgggc gagctcgaat 
      901 tcagatctgg ttaccactaa accagcctca agaacacccg aatggagtct ctaagctaca 
      961 taataccaac ttacacttta caaaatgttg tcccccaaaa tgtagccatt cgtatctgct 
     1021 cctaataaaa agaaagtttc ttcacattct aaaaaaaaaa aaaaaaaaaa aaaccccccc 
     1081 cccccccccc cccccccccc cccgatcccc ggggatccct ctctttttca acaaacttgc 
     1141 aaaaaagatt aaggaggaag gattgaaaac tggttcatca tggaaatgat tctcattgac 
     1201 taatacatca tctcacactt tcatgagttc ttccatttca aagactcact tctcctataa 
     1261 ccaccacaag ttgaatcaaa attttcaaat gttttcagga gtgtaaagaa gcatcatgta 
     1321 tacctgtgca ggcactagtc ctttacagat gaccatgctg atgtctcctt tcatctattt 
     1381 atttaaatat ttatttattt aactattttt attatttaaa ttatttttta tgtaatatca 
     1441 tgtgtgacct gcagcccaag ctcgaattcg taatcatggt catagctgtt tcctgtgtga 
     1501 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     1561 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     1621 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 
     1681 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     1741 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     1801 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     1861 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     1921 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     1981 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     2041 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     2101 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     2161 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     2221 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     2281 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 
     2341 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     2401 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     2461 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     2521 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     2581 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     2641 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     2701 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     2761 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     2821 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     2881 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     2941 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     3001 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     3061 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     3121 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     3181 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     3241 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     3301 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     3361 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     3421 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     3481 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     3541 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     3601 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 
     3661 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 
     3721 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 
     3781 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 
     3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatcga cgctctccct 
     3901 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg 
     3961 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg 
     4021 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc 
     4081 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 
     4141 ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt ggttcgctga 
     4201 ccatttccgg ggtgcggaac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg 
     4261 actctgacgg cagtttacga gagagatgat agggtctgct tcagtaagcc agatgctaca 
     4321 caattaggct tgtacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt 
     4381 atcatacaca tacgatttag gtgacactat agaatac 
//
