ID pSP64GSHIFNBNT standard; circular DNA; ; 4417 BP. DE LMBP 2789. CC Accession number: LMBP 2789 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 7-APR-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 11..53 FT /vntifkey="21" FT /label=5'\UTR\of\ß-globin FT /note="5' UTR of the X. laevis ß-globin gene" FT CDS 90..653 FT /vntifkey="4" FT /label=hIFNß FT /note="human interferon ß signal and mature sequence (signal seq.: 90-152)" FT misc_feature 654..853 FT /vntifkey="21" FT /label=3'\UTR\of\hIFNß FT misc_feature 910..1050 FT /vntifkey="21" FT /label=3'\UTR\of\ß-globin FT /note="3' UTR of the X. laevis ß-globin gene" FT terminator 1114..1445 FT /vntifkey="43" FT /label=hIFNalpha14\terminator FT /note="human interferon alpha 14 gene terminator" FT promoter complement(1522..1605) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(1697..2099) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(2640..3500) FT /vntifkey="4" FT /label=ampicillin FT promoter 4395..4411 FT /vntifkey="30" FT /label=SP6\promoter SQ Sequence 4417 BP; 1185 A; 1094 C; 1009 G; 1129 t; aagctagctt cttgttcttt ttgcagaagc tcagaataaa cgctcaactt tggcagatcc 60 caagcttggc caaaaaggcc tcgaggaaca tgaccaacaa gtgtctcctc caaattgctc 120 tcctgttgtg cttctccact acagctcttt ccatgagcta caacttgctt ggattcctac 180 aaagaagcag caattttcag tgtcagaagc tcctgtggca attgaatggg aggcttgaat 240 actgcctcaa ggacaggatg aactttgaca tccctgagga gattaagcag ctgcagcagt 300 tccagaagga ggacgccgca ttgaccatct atgagatgct ccagaacatc tttgctattt 360 tcagacaaga ttcatctagc actggctgga atgagactat tgttgagaac ctcctggcta 420 atgtctatca tcagataaac catctgaaga cagtcctgga agaaaaactg gagaaagaag 480 atttcaccag gggaaaactc atgagcagtc tgcacctgaa aagatattat gggaggattc 540 tgcattacct gaaggccaag gagtacagtc actgtgcctg gaccatagtc agagtggaaa 600 tcctaaggaa cttttacttc attaacagac ttacaggtta cctccgaaac tgaagatctc 660 ctagcctgtg cctctgggac tggacaattg cttcaagcat tcttcaacca gcagatgctg 720 tttaagtgac tgatggctaa tgtactgcat atgaaaggac actagaagat tttgaaattt 780 ttattaaatt atgagttatt tttatttatt taaattttat tttggaaaat aaattatttt 840 tggtgcaaaa gtccctcgag gcctagcggc cgcctagagg atccccgggc gagctcgaat 900 tcagatctgg ttaccactaa accagcctca agaacacccg aatggagtct ctaagctaca 960 taataccaac ttacacttta caaaatgttg tcccccaaaa tgtagccatt cgtatctgct 1020 cctaataaaa agaaagtttc ttcacattct aaaaaaaaaa aaaaaaaaaa aaaccccccc 1080 cccccccccc cccccccccc cccgatcccc ggggatccct ctctttttca acaaacttgc 1140 aaaaaagatt aaggaggaag gattgaaaac tggttcatca tggaaatgat tctcattgac 1200 taatacatca tctcacactt tcatgagttc ttccatttca aagactcact tctcctataa 1260 ccaccacaag ttgaatcaaa attttcaaat gttttcagga gtgtaaagaa gcatcatgta 1320 tacctgtgca ggcactagtc ctttacagat gaccatgctg atgtctcctt tcatctattt 1380 atttaaatat ttatttattt aactattttt attatttaaa ttatttttta tgtaatatca 1440 tgtgtgacct gcagcccaag ctcgaattcg taatcatggt catagctgtt tcctgtgtga 1500 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 1560 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 1620 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 1680 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 1740 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 1800 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 1860 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 1920 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 1980 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 2040 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 2100 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 2160 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 2220 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 2280 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 2340 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 2400 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 2460 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 2520 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 2580 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 2640 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 2700 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 2760 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 2820 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 2880 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 2940 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 3000 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 3060 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 3120 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 3180 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 3240 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 3300 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 3360 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 3420 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 3480 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 3540 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 3600 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 3660 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 3720 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 3780 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 3840 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatcga cgctctccct 3900 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg 3960 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg 4020 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc 4080 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg 4140 ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt ggttcgctga 4200 ccatttccgg ggtgcggaac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg 4260 actctgacgg cagtttacga gagagatgat agggtctgct tcagtaagcc agatgctaca 4320 caattaggct tgtacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt 4380 atcatacaca tacgatttag gtgacactat agaatac 4417 //