LOCUS       p9HP1B5B12              3604 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2794.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|p9HP1B5B12|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "p9HP1B5B12" 0 3604 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3604 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "3UTR" 21 0 1 402 829 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(402..829)") 
COMMENT        #4=(CFSignal (CObList) "mIL6" 4 0 1 830 1387 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(830..1387)") 
COMMENT        #5=(CFSignal (CObList) "5UTR" 21 0 1 1388 1425 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1388..1425)") 
COMMENT        #6=(CFSignal (CObList) "lac" 30 0 1 1426 1509 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1426..1509)") 
COMMENT        #7=(CFSignal (CObList) "ORI" 33 0 1 1595 2005 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1595..2005)") 
COMMENT        #8=(CFSignal (CObList) "AMP" 4 0 1 2544 3404 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2544..3404)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                         "DNA 'p9HP1B5B12'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
COMMENT                    #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Creation Date: ? (no data)" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3604 bp" 1) 
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COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                  "Annotations") 
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COMMENT                                       "Start: 830   End: 1387 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "fragment of the mouse interleukin 6 coding seq." 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   complement(830..1387)" 1))) "mIL6") 
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COMMENT                                       "Start: 2544  End: 3404 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   complement(2544..3404)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       "Start: 402   End: 829  (Complementary)" 
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COMMENT                                  #38=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of the mouse interleukin 6 gene" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #40=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(402..829)" 1))) "3UTR") 
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COMMENT                                       1) 
COMMENT                                  #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "5' UTR of the E. coli lacZ gene, incl. RBS" 
COMMENT                                       1) 
COMMENT                                  #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #45=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(1388..1425)" 1))) "5UTR"))) 
COMMENT                         "Misc. Feature (2 total)") 
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COMMENT                                       "Start: 1426  End: 1509 (Complementary)" 
COMMENT                                       1) 
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COMMENT                                       "E. coli lac operon promoter" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                         "Promoter Prokaryotic (1 total)") 
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COMMENT                                       "origin plasmid" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                         "Replication Origin (1 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     misc_feature    complement(402..829)
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the mouse interleukin 6 gene"
     CDS             complement(830..1387)
                     /vntifkey="4"
                     /label=mIL6
                     /note="fragment of the mouse interleukin 6 coding seq."
     misc_feature    complement(1388..1425)
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the E. coli lacZ gene, incl. RBS"
     promoter        complement(1426..1509)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(1595..2005)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2544..3404)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      966 a       821 c       827 g       990 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctttttttta aatataatat 
      421 aatttatttg tttgaagaca gtctaaacat tataaaaata catcacaaag atttctttaa 
      481 aaacatattt aaattagcaa ttcattgagg taaacttata cattccaaga aaccatctgg 
      541 ctaggtaaca gaatatttat atcaaataga tttttaggtt atcatttcaa aactataaca 
      601 tataacattt caagtgacac ttcataaaaa taataaatat caatcataaa ttaacttaaa 
      661 gtttacttat ttaaattatc aataaattaa aaataattaa aatagtgtcc caacattcat 
      721 attgtcagtt cttcgtagag aacaacataa gtcagatacc tgacaacagg atatattttc 
      781 tgaccacagt gaggaatgtc cacaaactga tatgcttagg cataacgcac taggtttgcc 
      841 gagtagatct caaagtgact tttagaaatt cttcaagtga tttcaagatg aattggatgg 
      901 tcttggtcct tagccactcc ttctgtgact ccagcttatc tgttaggaga gcattggaaa 
      961 ttggggtagg aaggactatt ttatgtaaat cttttacctc ttggttgaag atatgaatta 
     1021 gagtttctgt atctctctga aggactctgg ctttgtcttt cttgttatct tttaagttgt 
     1081 tcttcatgta ctccaggtag ctatggtact ccagaagacc agaggaaatt ttcaataggc 
     1141 aaatttcctg attatatcca gtttggtagc atccatcatt tctttgtatc tctggaagtt 
     1201 tcagattgtt ttctgcaagt gcatcatcgt tgttcataca atcagaattg ccattgcaca 
     1261 actcttttct catttccacg atttcccaga gaacatgtgt aattaagcct ccgacttgtg 
     1321 aagtggtata gacaggtctg ttgggagtgg tatcctctgt agccaagctt ggcgtaatca 
     1381 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 
     1441 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 
     1501 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 
     1561 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     1621 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     1681 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     1741 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     1801 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     1861 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     1921 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     1981 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     2041 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     2101 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     2161 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     2221 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     2281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     2341 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     2401 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     2461 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     2521 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     2581 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     2641 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     2701 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     2761 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     2821 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     2881 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     2941 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     3001 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     3061 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     3121 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     3181 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     3241 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     3301 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     3361 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     3421 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     3481 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     3541 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     3601 cgtc 
//
