LOCUS       pBluMGAPDHf             3454 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2800.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBluMGAPDHf|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBluMGAPDHf" 0 3454 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1952670563 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3454 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "f1ORI" 33 0 0 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4..459") 
COMMENT        #4=(CFSignal (CObList) "T7" 30 0 0 626 642 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "626..642") 
COMMENT        #5=(CFSignal (CObList) "GAPDH" 4 0 1 712 1147 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(712..1147)") 
COMMENT        #6=(CFSignal (CObList) "5UTR" 21 0 1 1148 1188 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1148..1188)") 
COMMENT        #7=(CFSignal (CObList) "T3" 30 0 1 1268 1284 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1268..1284)") 
COMMENT        #8=(CFSignal (CObList) "lac" 30 0 1 1348 1431 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1348..1431)") 
COMMENT        #9=(CFSignal (CObList) "ORI" 33 0 1 1525 1925 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1525..1925)") 
COMMENT        #10=(CFSignal (CObList) "AMP" 4 0 1 2466 3326 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2466..3326)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA 'pBluMGAPDHf'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3454 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 712   End: 1147 (Complementary)" 
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COMMENT                                        "fragment of the mouse GAPDH sequence" 1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   complement(712..1147)" 1))) "GAPDH") 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "phage T7 gene 10 promoter" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      4..459
                     /vntifkey="33"
                     /label=f1ORI
                     /note="phage f1 origin"
     promoter        626..642
                     /vntifkey="30"
                     /label=T7
                     /note="phage T7 gene 10 promoter"
     CDS             complement(712..1147)
                     /vntifkey="4"
                     /label=GAPDH
                     /note="fragment of the mouse GAPDH sequence"
     misc_feature    complement(1148..1188)
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the mouse GAPDH gene"
     promoter        complement(1268..1284)
                     /vntifkey="30"
                     /label=T3
                     /note="phage T3 promoter"
     promoter        complement(1348..1431)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(1525..1925)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2466..3326)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      813 a       894 c       869 g       878 t 
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcccgg gtgacaatct 
      721 tgagtgagtt gtcatatttc tcgtggttca cacccatcac aaacatgggg gcatcggcag 
      781 aaggggcgga gatgatgacc cgtttggctc cacccttcaa gtgggccccg gccttctcca 
      841 tggtggtgaa gacaccagta gactccacga catactcagc accggcctca ccccatttga 
      901 tgttagtggg gtctcgctcc tggaagatgg tgatgggctt cccgttgatg acaagcttcc 
      961 cattctcggc cttgactgtg ccgttgaatt tgccgtgagt ggagtcatac tggaacatgt 
     1021 agaccatgta gttgaggtca atgaaggggt cgttgatggc aacaatctcc actttgccac 
     1081 tgcaaatggc agccctggtg accaggcgcc caatacggcc aaatccgttc acaccgacct 
     1141 tcaccatttt gtctacggga cgaggctggc actgcacaag aagatgcgcc cgggaattcc 
     1201 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt 
     1261 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct 
     1321 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
     1381 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
     1441 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
     1501 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     1561 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     1621 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     1681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     1741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     1801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     1861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
     1921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     1981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     2041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     2101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
     2161 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     2221 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     2281 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     2341 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
     2401 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
     2461 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
     2521 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
     2581 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
     2641 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
     2701 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
     2761 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
     2821 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
     2881 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
     2941 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
     3001 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
     3061 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 
     3121 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
     3181 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
     3241 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
     3301 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
     3361 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     3421 ggggttccgc gcacatttcc ccgaaaagtg ccac 
//
