LOCUS       pBluhANCTNf             4791 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2911.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBluhANCTNf|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBluhANCTNf" 0 4791 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1952670563 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4791 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "f1ORI" 33 0 0 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4..459") 
COMMENT        #4=(CFSignal (CObList) "T7" 30 0 0 626 642 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "626..642") 
COMMENT        #5=(CFSignal (CObList) "Nctnf" 4 0 1 686 2515 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(686..2515)") 
COMMENT        #6=(CFSignal (CObList) "T3" 30 0 1 2605 2621 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2605..2621)") 
COMMENT        #7=(CFSignal (CObList) "lac" 30 0 1 2685 2768 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2685..2768)") 
COMMENT        #8=(CFSignal (CObList) "ORI" 33 0 1 2855 3265 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2855..3265)") 
COMMENT        #9=(CFSignal (CObList) "AMP" 4 0 1 3803 4663 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3803..4663)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                          "DNA 'pBluhANCTNf'" 1) 
COMMENT                     #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4791 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 686   End: 2515 (Complementary)" 
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COMMENT                                   #28=(CLinePar 
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COMMENT                                        "fragment of the human \346N-catenin coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   complement(686..2515)" 1))) "Nctnf") 
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COMMENT                                        "Start: 3803  End: 4663 (Complementary)" 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                   #44=(CLinePar 
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COMMENT                                        "phage T3 promoter" 1) 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                          "Replication Origin (2 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     rep_origin      4..459
                     /vntifkey="33"
                     /label=f1ORI
                     /note="phage f1 origin"
     promoter        626..642
                     /vntifkey="30"
                     /label=T7
                     /note="phage T7 gene 10 promoter"
     CDS             complement(686..2515)
                     /vntifkey="4"
                     /label=Nctnf
                     /note="fragment of the human æN-catenin coding sequence"
     promoter        complement(2605..2621)
                     /vntifkey="30"
                     /label=T3
                     /note="phage T3 promoter"
     promoter        complement(2685..2768)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2855..3265)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3803..4663)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1072 a      1270 c      1175 g      1274 t 
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 
      661 gccccccctc gaggtcgacg gtatcgatga actcattctc ctcaaacggt tgaggaacgt 
      721 tggcactcag ggcttcaatg gcaacctcta cttgttcagc gaagcgtggc atcactgttt 
      781 cagaaagcaa ttttgtagct tccaacacct tctcagtata aaccccagct tcatagttct 
      841 ccatctcagc attgatgatg tgtatgactc gagctgcccg gcccctgatg gcccctgcag 
      901 tccggtccag agtgtccaca tcgccctctt ggagggctat cacacacttg ttcacatcct 
      961 ccaagatgtg attttctgag acagagagga agtcatccac tgaggtgatg tcatccacgg 
     1021 cctctgtcaa cactcggacc tgcttctccc actggtcttt gaagacgtcc atgttatcct 
     1081 gagcaacttt gctctgtggc cgggcagcca gtgtcagagc ggcattgatg acctggggac 
     1141 acaggctgtc aatctgggtg gctgccatcc gaactaattt caccccttct tcattgttgg 
     1201 agatggaaca ggccaaattg gcaacctcta ccagtttgtt ggcatgctca cggaaaactt 
     1261 gggcatattc tttcacttcc ttttcatttc cgctctttgc agcctcaatg agaactagca 
     1321 aaggaacatt ggtttccagg aaagagtcag atatgtgatc catcactgct ttccgaagct 
     1381 gtctccttag atctcttgtt ttcttagtca tcttatcaat cgcaatgttg agaggatctc 
     1441 ctttttcttt ccttccagta ttattcatgt actcgctgag caggtcctgg agcgcctgcc 
     1501 gcacggcgtt gcactccgcc acgatcctct cgcgccggtc gtctcgcgtg caggaggagt 
     1561 cggccatcag cgctgcgccg ctgatgatgc tctccagcct ctcctccagg gacggccgga 
     1621 acctggcctc gctgaacgtc atggggtcca ggataatctt attgtcaaac tcattaagag 
     1681 ccgcagccag ctcgccgatg cccgtgtggc ccttggcttc gtcagtgggc gaggtagctt 
     1741 gagcagcatt ggagatgccg gcgatggcct cctggacttg tttgaacaca taatctcggt 
     1801 tggctctcgt agcggcgaca tctgggtggc ggagaaatgc ttgagaggcc gtgtacagca 
     1861 ttgtggcatt cttcttcaga gcccctcggg cggctgccat ctcatcccga cagtgaggat 
     1921 ccttcagctc ctgttgtctt cttgctgcta catagttaag tttcaccatc tttttcccaa 
     1981 actctttaaa acggtttgca aggtcttgct catttgtagc atttttgaca gcttccaggg 
     2041 cctcttccac aattttcaga tgggataaaa gtctcatgac atctgccatg tccgccagga 
     2101 tgagtaagcg tgtcaccgcg gagagcaaag cccttgccgc ccgtaccatg gtgccgcgct 
     2161 ttaccgacga gcaagggtca tctgcaaact cggaggaggc gatccgcatc gtctcacctt 
     2221 gtttgcgcac atcctctaca gcagccacca actcttcttt gagatcttga ctctccttag 
     2281 cgatctgttc acccttttcc aggaaattct gagtggcttg ctctacagag gcagctagta 
     2341 catgggcttt ctttgacctc cctttctttt taccagatgg gcctttgttg cttgtgttga 
     2401 caagtgtagt cacctgtgta acaagtggct ccaacagcct ttccactgtt agcgtccgga 
     2461 tttccaaact tttggggtcc catttcagaa tgataggtga agttgccgaa gtcatgctcc 
     2521 ctctgtgggg gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc 
     2581 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat 
     2641 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     2701 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 
     2761 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     2821 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     2881 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     2941 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     3001 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     3061 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     3121 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     3181 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     3241 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     3301 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     3361 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     3421 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     3481 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     3541 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     3601 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     3661 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     3721 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     3781 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     3841 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     3901 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     3961 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     4021 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     4081 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     4141 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     4201 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     4261 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     4321 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     4381 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     4441 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     4501 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     4561 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     4621 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     4681 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     4741 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c 
//
