LOCUS       pBRphoAE6Hf1v           4573 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2955.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRphoAE6Hf1v|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRphoAE6Hf1v" 0 4573 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1734435175 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4573 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            1 1 1 1 "31..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 196 555 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "196..555") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 556 861 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "556..861") 
COMMENT        #8=(CFSignal (CObList) "ORI" 33 0 1 2565 2965 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2565..2965)") 
COMMENT        #9=(CFSignal (CObList) "AMP" 4 0 1 3505 4365 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3505..4365)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA 'pBRphoAE6Hf1v'" 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4573 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 196   End: 555 " 1) 
COMMENT                                   #28=(CLinePar 
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COMMENT                                        "variable domain of E6H" 1) 
COMMENT                                   #29=(CLinePar 
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COMMENT                                        "Start: 556   End: 861 " 1) 
COMMENT                                   #33=(CLinePar 
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COMMENT                                        "Start: 3505  End: 4365 (Complementary)" 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #44=(CLinePar 
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COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
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COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
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COMMENT                                        "Start: 31    End: 65  " 1) 
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COMMENT                                        "E. coli hybrid tryptophan/lac operon promoter" 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     promoter        31..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             196..555
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             556..861
                     /vntifkey="4"
                     /label=CH1
                     /note="constant domain 1 of E6H"
     rep_origin      complement(2565..2965)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3505..4365)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1076 a      1255 c      1169 g      1073 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc ggatccaggt tcagctgcag cagtctggag ctgagctggc gaggcctggg 
      241 gcctcagtga agctgtcctg caaggcctct ggatacaccc tcacaagtta tggtataagt 
      301 tgggtgaagc agagaactgg acagggcctt gagtggattg gagaaattta tcctggaagt 
      361 ggaaattcgt acttcaatga gaagtttaag ggcaaggcca cactgactgt agacaaatcc 
      421 tccagcacag cctacttgca cctcagcagc ctgacatctg aggactctgc cgtctatttc 
      481 tgtgccggac ctagacaggt cgggctactc ccttttggtt actggggcca agggactctg 
      541 gtcactgcct ctgcagccaa aacaacaccc ccatcagtct atccactggc ccctgggtgt 
      601 ggagatacaa ctggttcctc cgtgactctg ggatgcctgg tcaagggcta cttccctgag 
      661 tcagtgactg tgacttggaa ctccggatcc ctgtccagca gtgtgcacac cttcccagct 
      721 ctcctgcagt ctggactcta cactatgagc agctcagtga ctgtcccctc cagcacctgg 
      781 ccaagccaga ccgttacctg cagtgttgct cacccagcca gcagcaccac ggtggacaaa 
      841 aaacttgagc ccagcgtcta gagtcgaccg atgcccttga gagccttcaa cccagtcagc 
      901 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc 
      961 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt 
     1021 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc 
     1081 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc 
     1141 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg 
     1201 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag 
     1261 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg 
     1321 gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc 
     1381 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc 
     1441 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc 
     1501 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa 
     1561 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca 
     1621 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc 
     1681 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat 
     1741 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa 
     1801 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc 
     1861 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac 
     1921 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc 
     1981 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat 
     2041 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca 
     2101 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg 
     2161 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 
     2221 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 
     2281 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 
     2341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 
     2401 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 
     2461 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 
     2521 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 
     2581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     2641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     2701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     2761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     2821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     2881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     2941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     3001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     3061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     3121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     3181 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     3241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     3301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     3361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     3421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     3481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     3541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     3601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     3661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     3721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     3781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 
     3841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     3901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     3961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     4021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     4081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 
     4141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     4201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     4261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     4321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     4381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     4441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     4501 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     4561 tcgtcttcaa gaa 
//
