LOCUS       pBRphoAE6L              4713 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2959.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRphoAE6L|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRphoAE6L" 0 4713 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4713 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "tac" 30 0 0 31 65 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "31..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VL" 4 0 0 192 512 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..512") 
COMMENT        #7=(CFSignal (CObList) "CL" 4 0 0 513 836 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "513..836") 
COMMENT        #8=(CFSignal (CObList) "3UTR" 21 0 0 837 1005 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "837..1005") 
COMMENT        #9=(CFSignal (CObList) "ORI" 33 0 1 2700 3100 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2700..3100)") 
COMMENT        #10=(CFSignal (CObList) "AMP" 4 0 1 3645 4505 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3645..4505)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                          "DNA 'pBRphoAE6L'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4713 bp" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                   #28=(CLinePar 
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COMMENT                                        "Start: 192   End: 512 " 1) 
COMMENT                                   #29=(CLinePar 
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COMMENT                                        "variable domain of E6L" 1) 
COMMENT                                   #30=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #31=(CLinePar 
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COMMENT                                        "   192..512" 1))) "VL") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 513   End: 836 " 1) 
COMMENT                                   #34=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain of E6L" 1) 
COMMENT                                   #35=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "Start: 3645  End: 4505 (Complementary)" 
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COMMENT                                   #39=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                          "CDS (3 total)") 
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COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #45=(CLinePar 
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COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   66..128" 1))) "5UTR") 
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COMMENT                                        "Start: 129   End: 191 " 1) 
COMMENT                                   #50=(CLinePar 
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COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
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COMMENT                                        "Start: 837   End: 1005" 1) 
COMMENT                                   #55=(CLinePar 
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COMMENT                                        "3' UTR of E6L" 1) 
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COMMENT                                   #57=(CLinePar 
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COMMENT                          "Misc. Feature (3 total)") 
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COMMENT                                        "E. coli hybrid tryptophan/lac operon promoter" 
COMMENT                                        1) 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     promoter        31..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..512
                     /vntifkey="4"
                     /label=VL
                     /note="variable domain of E6L"
     CDS             513..836
                     /vntifkey="4"
                     /label=CL
                     /note="constant domain of E6L"
     misc_feature    837..1005
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of E6L"
     rep_origin      complement(2700..3100)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3645..4505)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1139 a      1297 c      1163 g      1114 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc ggacatccag atgactcagt ctccagcctc cctatccgtt tctgtgggag 
      241 aatctgtcac catcacatgt cgagcaagtg agaatattta cagtaattta gcatggtatc 
      301 agcagaaaca gggaaaatct cctcagctcc ttgtctatgt tgcgacaaag ttagtagatg 
      361 gtgtgccatc aaggttcagt ggcagtggat caggcacaca gtattccctc aagatcaaca 
      421 gcctgcagtc tgaagatttt gggagttatt actgtcaaca tttttgggac actccattca 
      481 cgttcggctc ggggacaaag ttggaaatga aacgggctga tgctgcacca actgtatcca 
      541 tcttcccacc atccagtgag cagttaacat ctggaggtgc ctcagtcgtg tgcttcttga 
      601 acaacttcta ccccaaagac atcaatgtca agtggaagat tgatgggagt gaacgacaaa 
      661 atggcgtcct gaacagttgg actgatcagg acagcaaaga cagcacctac agcatgagca 
      721 gcaccctcac gttgaccaag gacgagtatg aacgacataa cagctatacc tgtgaggcca 
      781 ctcacaagac atcaacttca cccattgtca agagcttcaa caggaatgag tgttagagac 
      841 aaaggtcctg agacgccacc accagctccc cagctccatc ctatcttccc ttctaaggtc 
      901 ttggaggctt ccccacaagc gacctaccac tgttgcggtg ctccaaacct cctccccacc 
      961 tccttctcct cctcctccct ttccttcccc cccccccccc ccgtcgaccg atgcccttga 
     1021 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 
     1081 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 
     1141 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 
     1201 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 
     1261 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 
     1321 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 
     1381 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 
     1441 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg 
     1501 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg 
     1561 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 
     1621 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc 
     1681 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat 
     1741 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc 
     1801 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 
     1861 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 
     1921 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 
     1981 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 
     2041 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 
     2101 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 
     2161 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 
     2221 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 
     2281 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 
     2341 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 
     2401 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     2461 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     2521 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 
     2581 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 
     2641 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 
     2701 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     2761 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     2821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     2881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     2941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     3001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     3061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     3121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     3181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     3241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     3301 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     3361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     3421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     3481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     3541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     3601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     3661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     3721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     3781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     3841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     3901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     3961 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     4021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     4081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     4141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     4201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     4261 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     4321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     4381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     4441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     4501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     4621 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 
     4681 aggcgtatca cgaggccctt tcgtcttcaa gaa 
//
