LOCUS       pBRsE6Hf1sE6L           5565 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2961.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRsE6Hf1sE6L|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRsE6Hf1sE6L" 0 5565 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1952937059 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5565 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            1 1 1 1 "31..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 842 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..842") 
COMMENT        #8=(CFSignal (CObList) "tac" 30 0 0 883 917 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "883..917") 
COMMENT        #9=(CFSignal (CObList) "5UTR" 21 0 0 918 980 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "918..980") 
COMMENT        #10=(CFSignal (CObList) "sPHOA" 21 0 0 981 1043 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "981..1043") 
COMMENT        #11=(CFSignal (CObList) "VL" 4 0 0 1044 1364 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1044..1364") 
COMMENT        #12=(CFSignal (CObList) "CL" 4 0 0 1365 1688 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1365..1688") 
COMMENT        #13=(CFSignal (CObList) "3UTR" 21 0 0 1689 1857 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1689..1857") 
COMMENT        #14=(CFSignal (CObList) "ORI" 33 0 1 3555 3955 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3555..3955)") 
COMMENT        #15=(CFSignal (CObList) "AMP" 4 0 1 4497 5357 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4497..5357)")) (CObList) 
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COMMENT                          "DNA 'pBRsE6Hf1sE6L'" 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5565 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 192   End: 551 " 1) 
COMMENT                                   #34=(CLinePar 
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COMMENT                                        "variable domain of E6H" 1) 
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COMMENT                                   #45=(CLinePar 
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COMMENT                                        "Start: 1365  End: 1688" 1) 
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COMMENT                                        "Start: 4497  End: 5357 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                          "CDS (5 total)") 
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COMMENT                                        "Start: 66    End: 128 " 1) 
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COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
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COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                   3.74328 13.9386 0 -10 2.57829 1.16519 #243#) 
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COMMENT                                   8.01555 12.1963 0 -10 3.19807 1.16519 #245#) 
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FEATURES             Location/Qualifiers
     promoter        31..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..842
                     /vntifkey="4"
                     /label=CH1
                     /note="constant domain 1 of E6H"
     promoter        883..917
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    918..980
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    981..1043
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             1044..1364
                     /vntifkey="4"
                     /label=VL
                     /note="variable domain of E6L"
     CDS             1365..1688
                     /vntifkey="4"
                     /label=CL
                     /note="constant domain of E6L"
     misc_feature    1689..1857
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR 0F E6L"
     rep_origin      complement(3555..3955)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4497..5357)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1344 a      1536 c      1378 g      1307 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctacact atgagcagct cagtgactgt cccctccagc acctggccaa 
      781 gccagaccgt tacctgcagt gttgctcacc cagccagcag caccacggtg gacaaaaaac 
      841 ttgagcccag cgtctagagt cgagcttact ccccatcccc ctgttgacaa ttaatcatcg 
      901 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa caggatccgc 
      961 ggatccgtgg agaaaataaa gtgaaacaaa gcactattgc actggcactc ttaccgttac 
     1021 tgtttacccc tgtgacaaaa gcggacatcc agatgactca gtctccagcc tccctatccg 
     1081 tttctgtggg agaatctgtc accatcacat gtcgagcaag tgagaatatt tacagtaatt 
     1141 tagcatggta tcagcagaaa cagggaaaat ctcctcagct ccttgtctat gttgcgacaa 
     1201 agttagtaga tggtgtgcca tcaaggttca gtggcagtgg atcaggcaca cagtattccc 
     1261 tcaagatcaa cagcctgcag tctgaagatt ttgggagtta ttactgtcaa catttttggg 
     1321 acactccatt cacgttcggc tcggggacaa agttggaaat gaaacgggct gatgctgcac 
     1381 caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt gcctcagtcg 
     1441 tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag attgatggga 
     1501 gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa gacagcacct 
     1561 acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat aacagctata 
     1621 cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc aacaggaatg 
     1681 agtgttagag acaaaggtcc tgagacgcca ccaccagctc cccagctcca tcctatcttc 
     1741 ccttctaagg tcttggaggc ttccccacaa gcgacctacc actgttgcgg tgctccaaac 
     1801 ctcctcccca cctccttctc ctcctcctcc ctttccttcc cccccccccc ccccgtcgac 
     1861 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta 
     1921 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag 
     1981 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt 
     2041 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca 
     2101 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct 
     2161 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg 
     2221 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg 
     2281 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg 
     2341 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat 
     2401 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga 
     2461 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc 
     2521 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca 
     2581 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt 
     2641 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg 
     2701 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga 
     2761 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt 
     2821 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat 
     2881 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca 
     2941 ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc 
     3001 acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc 
     3061 tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt 
     3121 gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac 
     3181 gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct 
     3241 tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga 
     3301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 
     3361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat 
     3421 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag 
     3481 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 
     3541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     3601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     3661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 
     3721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 
     3781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 
     3841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 
     3901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 
     3961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 
     4021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 
     4081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 
     4141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 
     4201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 
     4261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 
     4321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 
     4381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 
     4441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 
     4501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 
     4561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 
     4621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 
     4681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 
     4741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 
     4801 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 
     4861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 
     4921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 
     4981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 
     5041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 
     5101 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 
     5161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 
     5221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 
     5281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 
     5341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 
     5401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 
     5461 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 
     5521 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaa 
//
