LOCUS       pBRsE6Hf2sE6L           5667 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2962.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRsE6Hf2sE6L|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRsE6Hf2sE6L" 0 5667 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1952937059 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5667 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "tac" 30 0 0 31 65 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "31..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 842 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..842") 
COMMENT        #8=(CFSignal (CObList) "HINGE" 4 0 0 843 908 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "843..908") 
COMMENT        #9=(CFSignal (CObList) "CH2f" 4 0 0 909 935 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "909..935") 
COMMENT        #10=(CFSignal (CObList) "tac" 30 0 0 985 1019 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "985..1019") 
COMMENT        #11=(CFSignal (CObList) "5UTR" 21 0 0 1020 1082 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1020..1082") 
COMMENT        #12=(CFSignal (CObList) "sPHOA" 21 0 0 1083 1145 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1083..1145") 
COMMENT        #13=(CFSignal (CObList) "VL" 4 0 0 1146 1466 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1146..1466") 
COMMENT        #14=(CFSignal (CObList) "CL" 4 0 0 1467 1790 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1467..1790") 
COMMENT        #15=(CFSignal (CObList) "3UTR" 21 0 0 1791 1959 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1791..1959") 
COMMENT        #16=(CFSignal (CObList) "ORI" 33 0 1 3660 4060 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3660..4060)") 
COMMENT        #17=(CFSignal (CObList) "AMP" 4 0 1 4599 5459 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4599..5459)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                   5 "" 0 4) 
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COMMENT                    (CObjectList 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pBRsE6Hf2sE6L'" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5667 bp" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #30=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #34=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 192 (3 #6# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #35=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 551 " 1) 
COMMENT                                   #36=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6H" 1) 
COMMENT                                   #37=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   192..551" 1))) "VH") 
COMMENT                            #39=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 552   End: 842 " 1) 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 1 of E6H" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   552..842" 1))) "CH1") 
COMMENT                            #44=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 843 (3 #8# 0) 1 2 2 0 327) 
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COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 843   End: 908 " 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "hinge domain of E6H" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   843..908" 1))) "HINGE") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 909 (3 #9# 0) 1 2 2 0 327) 
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COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 909   End: 935 " 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "fragment of the constant domain 2 of E6H" 
COMMENT                                        1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   909..935" 1))) "CH2f") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1146 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1146  End: 1466" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6L" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1146..1466" 1))) "VL") 
COMMENT                            #59=(CFolderPar 
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COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1467  End: 1790" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain of E6L" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1467..1790" 1))) "CL") 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4599 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4599  End: 5459 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4599..5459)" 1))) "AMP"))) 
COMMENT                          "CDS (7 total)") 
COMMENT                     #69=(CFolderPar 
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COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   66..128" 1))) "5UTR") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 129 (3 #5# 0) 1 2 2 0 194) 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "Start: 129   End: 191 " 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
COMMENT                                   #78=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #79=(CLinePar 
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COMMENT                                        "   129..191" 1))) "sPHOA") 
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COMMENT                                        "Start: 1020  End: 1082" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
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COMMENT                                        "   1020..1082" 1))) "5UTR") 
COMMENT                            #85=(CFolderPar 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                        "Start: 1083  End: 1145" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
COMMENT                                   #88=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
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COMMENT                                        "   1083..1145" 1))) "sPHOA") 
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COMMENT                                        "Start: 1791  End: 1959" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR 0F E6L" 1) 
COMMENT                                   #93=(CLinePar 
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COMMENT                                   #94=(CLinePar 
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COMMENT                                        "   1791..1959" 1))) "3UTR"))) 
COMMENT                          "Misc. Feature (5 total)") 
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COMMENT                                        "Start: 31    End: 65  " 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli hybrid tryptophan/lac operon promoter" 
COMMENT                                        1) 
COMMENT                                   #99=(CLinePar 
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COMMENT                                   #100=(CLinePar 
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COMMENT                                          "Start: 985   End: 1019" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "E. coli hybrid tryptophan/lac operon promoter" 
COMMENT                                          1) 
COMMENT                                    #104=(CLinePar 
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COMMENT                                    #105=(CLinePar 
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COMMENT                                          "   985..1019" 1))) "tac"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                            (CObjectList 
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COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3660  End: 4060 (Complementary)" 
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COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "origin plasmid" 1) 
COMMENT                                     #110=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #111=(CLinePar 
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COMMENT                                           "   complement(3660..4060)" 1))) 
COMMENT                                   "ORI"))) "Replication Origin (1 total)"))) 
COMMENT                   "Feature Map")))) 
COMMENT       (CGraphView 
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COMMENT                1 2 "?" 0) 
COMMENT          #113=(CWidgetStyle "Mark 1" 0 0 1 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #118=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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FEATURES             Location/Qualifiers
     promoter        31..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..842
                     /vntifkey="4"
                     /label=CH1
                     /note="constant domain 1 of E6H"
     CDS             843..908
                     /vntifkey="4"
                     /label=HINGE
                     /note="hinge domain of E6H"
     CDS             909..935
                     /vntifkey="4"
                     /label=CH2f
                     /note="fragment of the constant domain 2 of E6H"
     promoter        985..1019
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    1020..1082
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    1083..1145
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             1146..1466
                     /vntifkey="4"
                     /label=VL
                     /note="variable domain of E6L"
     CDS             1467..1790
                     /vntifkey="4"
                     /label=CL
                     /note="constant domain of E6L"
     misc_feature    1791..1959
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR 0F E6L"
     rep_origin      complement(3660..4060)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4599..5459)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1370 a      1571 c      1397 g      1329 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctacact atgagcagct cagtgactgt cccctccagc acctggccaa 
      781 gccagaccgt tacctgcagt gttgctcacc cagccagcag caccacggtg gacaaaaaac 
      841 ttgagcccag cgggcccact tcaacaatca acccctgtcc tccatgcaag gagtgtcaca 
      901 aatgcccagc tcctaacctc gacttagtta actaagcttg gctctagcgg taccgctaga 
      961 gtcgagctta ctccccatcc ccctgttgac aattaatcat cggctcgtat aatgtgtgga 
     1021 attgtgagcg gataacaatt tcacacagga aacaggatcc gcggatccgt ggagaaaata 
     1081 aagtgaaaca aagcactatt gcactggcac tcttaccgtt actgtttacc cctgtgacaa 
     1141 aagcggacat ccagatgact cagtctccag cctccctatc cgtttctgtg ggagaatctg 
     1201 tcaccatcac atgtcgagca agtgagaata tttacagtaa tttagcatgg tatcagcaga 
     1261 aacagggaaa atctcctcag ctccttgtct atgttgcgac aaagttagta gatggtgtgc 
     1321 catcaaggtt cagtggcagt ggatcaggca cacagtattc cctcaagatc aacagcctgc 
     1381 agtctgaaga ttttgggagt tattactgtc aacatttttg ggacactcca ttcacgttcg 
     1441 gctcggggac aaagttggaa atgaaacggg ctgatgctgc accaactgta tccatcttcc 
     1501 caccatccag tgagcagtta acatctggag gtgcctcagt cgtgtgcttc ttgaacaact 
     1561 tctaccccaa agacatcaat gtcaagtgga agattgatgg gagtgaacga caaaatggcg 
     1621 tcctgaacag ttggactgat caggacagca aagacagcac ctacagcatg agcagcaccc 
     1681 tcacgttgac caaggacgag tatgaacgac ataacagcta tacctgtgag gccactcaca 
     1741 agacatcaac ttcacccatt gtcaagagct tcaacaggaa tgagtgttag agacaaaggt 
     1801 cctgagacgc caccaccagc tccccagctc catcctatct tcccttctaa ggtcttggag 
     1861 gcttccccac aagcgaccta ccactgttgc ggtgctccaa acctcctccc cacctccttc 
     1921 tcctcctcct ccctttcctt cccccccccc ccccccgtcg accgatgccc ttgagagcct 
     1981 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 
     2041 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg 
     2101 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa 
     2161 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga 
     2221 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg 
     2281 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga 
     2341 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc 
     2401 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg 
     2461 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc 
     2521 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct 
     2581 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca 
     2641 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc 
     2701 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt 
     2761 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg 
     2821 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc 
     2881 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac 
     2941 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct 
     3001 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt 
     3061 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg 
     3121 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat 
     3181 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac 
     3241 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa 
     3301 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga 
     3361 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 
     3421 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 
     3481 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 
     3541 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 
     3601 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 
     3661 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     3721 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     3781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     3841 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     3901 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     3961 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     4021 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     4081 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     4141 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     4201 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     4261 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     4321 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     4381 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     4441 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     4501 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     4561 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     4621 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     4681 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     4741 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     4801 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     4861 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc 
     4921 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     4981 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     5041 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     5101 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     5161 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg 
     5221 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     5281 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     5341 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     5401 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     5461 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     5521 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     5581 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 
     5641 atcacgaggc cctttcgtct tcaagaa 
//
